CAS: 65-47-4; Cytidine-5'-Triphosphate

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CAS号63-39-8 尿苷-5'-三磷酸 | CAS号63-37-6 胞苷酸 | CAS号63-38-7 胞苷5'-二磷酸酯 | CAS号82913-19-7 2',3',5'-tri-O-... | CAS号58-96-8 尿苷 | CAS号4105-38-8 2,3,5-三乙酰尿苷 | CAS号56787-28-1 2',3',5'-TRI-O-... | CAS号65-46-3 胞嘧啶核苷 | CAS号56-65-5 5'-三磷酸腺苷 | CAS号84651-07-0 C2'p5'C2'p5'C | CAS号16511-83-4 [2']cytidylic a... | CAS号65-46-3 胞嘧啶核苷 | CAS号63-37-6 胞苷酸 | CAS号66-22-8 尿嘧啶 | CAS号63-38-7 胞苷5'-二磷酸酯 | CAS号3063-71-6 唾液 | CAS号3616-08-8 胞苷-3ˊ,5ˊ-环一磷酸 | CAS号1256-10-6 Cytidine 5'-(tr...

胞苷置于吡啶,水,碘体系中,用 N,N-二甲基甲酰胺 作为反应溶剂,化学反应 3.75H,反应生成 胞苷-5'-三磷酸
参考文献:一种核苷四磷酸的合成方法
标题:一种核苷四磷酸的合成方法
摘要:本发明公开了一种核苷四磷酸的合成方法.所述方法包括以下步骤:将核苷与环状磷酸化试剂发生选择性磷酸化反应,再经氧化,水解开环,即得所述核苷四磷酸.所述环状磷酸化试剂的结构如式i所示:式i.本方法将核苷在该高选择性磷酸化试剂作用下,选择性地反应生成5＇‑核苷四磷酸,在此过程中并不需要将3＇‑oh(以及2＇‑oh)保护起来,即能有效抑制3＇(以及2＇‑)四磷酸的反应生成.本发明合成的核苷四磷酸在dna测序,标记,延伸等生物学领域具有广泛用途,目前其销售价格昂贵,合成方法复杂,反应选择性差;而本发明提供的合成方法选择性好,容易分离纯化,且所需实验条件简单,合成过程均为常规化学反应,可用于大规模推广使用.

专利信息

专利号:US-2023235372-A1
优先权日:2020-06-12
标题:Ab-initio, template-independent synthesis of nucleic acids using thermostable enzymes
发明人：RANDRIANJATOVO-GBALOU IRINA; SAID AHMED; RAHIER RENAUD
权利人:SYNHELIX
摘要:The invention relates to the field of nucleic acid synthesis or sequencing, more specifically to methods for ab-initio synthesis of nucleic acids, comprising contacting a nucleotide with a free 3′-hydroxyl group, with at least one nucleoside triphosphate, or a combination of nucleoside triphosphates, in the presence of an archaeal DNA primase or a functionally active fragment and/or variant thereof, thereby covalently binding said nucleoside triphosphate to the free 3′-hydroxyl group of the nucleotide. It also relates to isolated functionally active fragments of archaeal DNA primases which are capable of both ab-initio single-stranded nucleic acid synthesis activity and template-independent terminal nucleotidyl transferase activity.

专利号:US-6440703-B1
优先权日:1997-12-01
标 题 :Enzymatic synthesis of gangliosides
发明人：DEFREES SHAWN
权利人:NEOSE TECHNOLOGIES INC
摘要:This invention provides methods for practical in vitro synthesis of gangliosides and other glycolipids. The synthetic methods typically involve enzymatic synthesis, or a combination of enzymatic and chemical synthesis. One or more of the enzymatic steps is preferably carried out in the presence of an organic solvent.

专利号:US-5374541-A
优先权日:1993-05-04
标 题 :Combined use of β-galactosidase and sialyltransferase coupled with in situ regeneration of CMP-sialic acid for one pot synthesis of oligosaccharides
发明人：WONG CHI-HUEY; GAETA FEDERICO C A
权利人:SCRIPPS RESEARCH INST; CYTEL CORP
摘要:A single reaction vessel process for the synthesis of a sialylated galactoside is disclosed. The synthesis utilizes a β-galactosidase to catalyze the reaction of a galactose-containing substrate and an acceptor to form a new galactosyl glycoside that is then sialylated using a cyclic multienzyme synthesis system to form CMP-sialic acid that sialylates the formed galactosyl glycoside in the presence of an α-sialyltransferase. The value of K m /V max for the formed galactosyl glycoside as a substrate for the α-sialyltransferase is less than one-third the K m /V max value for the galactose-containing substrate for that α-sialyltransferase.

专利号:WO-9640901-A1
优先权日:1995-06-07
标题 :Template and primer based synthesis of enzymatically cleavable oligonucleotides
发明人：DATTAGUPTA NANIBHUSHAN; KACIAN DANIEL LOUIS; LACKEY DAVID BRUCE
权利人:GEN PROBE INC
摘要:The present invention relates to methods of enzymatically synthesizing chirally pure oligonucleotides. In particular, it relates to methods of enzymatically synthesizing oligonucleotides using optionally digestible templates which are capable of directing the synthesis of an oligonucleotide product which can optionally be cleaved into two or more individual oligonucleotides, optionally using 3'-ribonucleotide primers. In this manner, more than one oligonucleotide having the same or different length and/or sequence can be simultaneously synthesized. The oligonucleotides optionally have at least one phosphorothioate linkage, and contain certain restriction endonuclease recognition sites herein identified as cleavable within phosphorothioate oligonucleotides, thus facilitating the separation and purification of the oligonucleotides after synthesis.

专利号:US-2023235373-A1
优先权日:2020-06-12
标 题:Controlled template-independent synthesis of nucleic acids using thermostable enzymes
发明人：RANDRIANJATOVO-GBALOU IRINA; SAID AHMED; RAHIER RENAUD
权利人:SYNHELIX
摘要:The invention relates to methods for template-independent synthesis of nucleic acids, comprising iteratively contacting an initiator sequence comprising a 3′-end nucleotide with a free 3′-hydroxyl group, with at least one nucleoside triphosphate, or a combination of nucleoside triphosphates, in the presence of an archaeal DNA primase or a functionally active fragment and/or variant thereof, thereby covalently binding said nucleoside triphosphate to the free 3-hydroxyl group of the 3-end nucleotide. It also relates to isolated functionally active fragments of archaeal DNA primases which are capable of template-independent terminal nucleotidyl transferase activity but are devoid of a template-independent primase activity.

专利号:US-11858953-B2
优先权日:2018-04-04
标 题:Compositions and methods for synthesis of phosphorylated molecules
发明人：CHAPUT JOHN; LIAO JEN-YU; BALA SAIKAT
权利人:UNIV CALIFORNIA
摘要:The invention provides compositions and methods for synthesis of phosphorylated organic compounds, including nucleoside triphosphates.