CAS: 150810-69-8; 6-Carboxytetramethylrhodamine N-Succinimidyl Ester

📜6-羧基四甲基罗丹明,丁二酰亚胺三氟乙酸酯 以 N,N-二甲基甲酰胺 用作溶剂,以85%的收率获得6-羧基四甲基罗丹明琥珀酰亚胺酯
参考文献:一种罗丹明活化酯的制备方法
标题:一种罗丹明活化酯的制备方法
摘要:本发明提供了一种罗丹明活化酯的制备方法,包括如下步骤:首先,将n‑羟基琥珀酰亚胺和三氟乙酸酐反应制备出三氟乙酸‑n‑羟基琥珀酰亚胺酯;其次,将罗丹明类染料和三氟乙酸‑n‑羟基琥珀酰亚胺酯溶解于有机溶剂中,充分反应,去除有机溶剂,提纯,制备出纯净度高的罗丹明活化酯.应用本申请所述的制备方法,副产物少,制备出的罗丹明活化酯不含有dcu杂质,产品纯度高,产率高,降低了生产成本.

专利信息

专利号:US-8071132-B2
优先权日:2004-06-01
标 题:Unagglomerated core/shell nanocomposite particles
发明人：ADAIR JAMES H; ROUSE SARAH M; WANG JUN; KESTER MARK; SIEDLECKI CHRISTOPHER; WHITE WILLIAM B; VOGLER ERWIN; SNYDER ALAN; PANTANO CARLO G; RUIZ-VELASCO VICTOR; SINOWAY LAWRENCE
权利人:ADAIR JAMES H; ROUSE SARAH M; WANG JUN; KESTER MARK; SIEDLECKI CHRISTOPHER; WHITE WILLIAM B; VOGLER ERWIN; SNYDER ALAN; PANTANO CARLO G; RUIZ-VELASCO VICTOR; SINOWAY LAWRENCE; PENN STATE RES FOUND
摘要:The present invention provides a method for the synthesis of unagglomerated, highly dispersed, stable core/shell nanocomposite particles comprised of preparing a reverse micelle microemulsion that contains nanocomposite particles, treating the microemulsion with a silane coupling agent, breaking the microemulsion to form a suspension of the nanocomposite particles by adding an acid/alcohol solution to the microemulsion that maintains the suspension of nanocomposite particles at a pH of between about 6 and 7, and simultaneously washing and dispersing the suspension of nanocomposite particles, preferably with a size exclusion HPLC system modified to ensure unagglomeration of the nanocomposite particles. The primary particle size of the nanocomposite particles can range in diameter from between about 1 to 100 nm, preferably from between about 10 to 50 nm, more preferably about 10 to 20 nm, and most preferably about 20 nm.

专利号:BR-PI0719923-B1
优先权日:2006-12-05
标 题:METHODS FOR SEQUENCING A TARGET NUCLEIC ACID, FOR CONVERTING A NON-NATURAL OCCURRING COMPONENT TO A NUCLEIC ACID MOLECULE TO A NATURAL OCCURRING COMPONENT, TO COMPLETE A SYNTHESIS OF NUCLIC ACID TO DEGER, AND TO SANITATION TO SANITATION AND COMPOUNDS

专利号:US-8969535-B2
优先权日:2006-12-05
标 题:Photocleavable labeled nucleotides and nucleosides and methods for their use in DNA sequencing
发明人：WU WEIDONG; LITOSH VLADISLAV A; STUPI BRIAN P; METZKER MICHAEL L
权利人:WU WEIDONG; LITOSH VLADISLAV A; STUPI BRIAN P; METZKER MICHAEL L; LASERGEN INC
摘要:Provided are novel nucleotides, nucleoside, and their derivatives described herein, that can be used in DNA sequencing technology and other types of DNA analysis. In one embodiment, the nucleotide or nucleoside with an unprotected 3′-OH group is derivatized at the nucleobase to include a fluorescent dye attached via a linker to a photocleavable terminating group. The photocleavable-fluorescent group is designed to terminate DNA synthesis as well as be cleaved so that DNA oligomers can be sequenced efficiently in a parallel format. The design of such rapidly cleavable fluorescent groups on nucleotides and nucleosides can enhance the speed and accuracy of sequencing of large oligomers of DNA in parallel, to allow rapid whole genome sequencing, and the identification of polymorphisms and other valuable genetic information, as well as allowing further manipulation and analysis of nucleic acid molecules in their native state following cleavage of the fluorescent group.

专利号:US-7759470-B2
优先权日:2006-02-20
标 题 :Labeling reagent
发明人：HEINDL DIETER; BERGMANN FRANK; JOSEL HANS-PETER; KESSLER DIRK; LAMPERT GUNTER; WEILKE CHRISTIAN
权利人:ROCHE DIAGNOSTICS OPERATIONS
摘要:The current invention restates substituted indole nucleosides as both terminal as well as internal building blocks of labeled oligonucleotide probes for the detection, analysis and quantitation of nucleic acids. The substituent comprises a linker and a detectable group or a linker and a reactive group for post synthesis coupling. These modified nucleosides grant access to a wide application area. These new substituted indole nucleosides can be used as labeling reagents for the facile preparation of e.g. optimized hybridization probes, Taqman-probes, or molecular beacon probes.