CAS: 519-21-1; 3-Dimethylamino-3,6-Dideoxy-β-D-Glucopyranose

专利信息

专利号:US-2008044860-A1
优先权日:2003-11-28
标题 :Polyketides and Their Synthesis
发明人：GAISSER SABINE; HAYDOCK STEPHEN FREDERICK; LEADLAY PETER FRANCIS; MCARTHUR HAMISH ALASTAIR IRVIN
权利人:GAISSER SABINE; HAYDOCK STEPHEN FREDERICK; LEADLAY PETER FRANCIS; MCARTHUR HAMISH ALASTAIR IRVIN
摘要:Macrolides particularly erythromycins and azithromycins, having O-mycaminosyl or O-angolosaminyl groups, particularly at the 5 -position, are produced using a gene cassette comprising a combination of genes which, in an appropriate strain background, are able to direct the synthesis of mycaminose or angolosamine and to direct its subsequent transfer to an aglycone or pseudoaglycone. Synthetic genes may comprise one or more of angMIII, angMI, angB, angAI, angAII, angorf14, angorf4, tylMIII, tylMI, tylB, tylAI, tylAII, eryCVI, spnO, eryBVI, eryK, tyl Ia and ery G. Glycosyltransfer genes may comprise one or more of eryCIII, tylMII, angMII, desVII, eryBV, spnP and midI.

专利号:US-2002192767-A1
优先权日:1999-10-13
标题 :Biosynthesis of polyketide synthase substrates
发明人：KHOSLA CHAITAN; PFEIFER BLAINE
摘要:The use of enzymes which catalyze the production of starter and extender units for polyketides is described. In addition, modified loading modules are described, which can accept a variety of starting units such as substituted benzoates, and which can be used to generate substituted derivatives of natural products. These enzymes may be used to enhance the yield of polyketides that are natively produced or polyketides that are rationally designed. By using these techniques, the synthesis of a complete polyketide has been achieved in E. coli . Production can be enhanced in microbial organisms of polyketides and other secondary metabolites by delaying production until after exponential phase and by maintaining relatively constant nutrient levels in the medium. In addition, polyketide production can be enhanced by providing an expression system for a thioesterase II. Thus, by modifying the host organism and changing the culture conditions, synthesis of a secondary metabolite can be enhanced. The present invention also results in a host organism with desirable characteristics to be used in the production of such polyketides and to assess the results of gene shuffling.

专利号:WO-02068613-A1
优先权日:2001-02-28
标题 :Biosynthesis of polyketide synthase substrates
发明人：KHOSLA CHAITAN; PFEIFER BLAINE
权利人:UNIV LELAND STANFORD JUNIOR; KHOSLA CHAITAN; PFEIFER BLAINE
摘要:The use of enzymes which catalyze the production of starter and extender units for polyketides is described. In addition, modified loading modules are described, which can accept a variety of starting units such as substituted benzoates, and which can be used to generate substituted derivatives of natural products. These enzymes may be used to enhance the yield of polyketides that are natively produced or polyketides that are rationally designed. By using these techniques, the synthesis of a complete polyketide has been achieved in E. coli. Production can be enhanced in microbial organisms of polyketides and other secondary metabolites by delaying production until after exponential phase and by maintaining relatively constant nutrient levels in the medium. In addition, polyketide production can be enhanced by providing an expression system for a thioesterase II. Thus, by modifying the host organism and changing the culture conditions, synthesis of a secondary metabolite can be enhanced. The present invention also results in a host organism with desirable characteristics to be used in the production of such polyketides and to assess the results of gene shuffling.

专利号:US-11008358-B2
优先权日:2015-03-25
标题:Synthesis of desosamines
发明人：MYERS ANDREW G; ZHANG ZIYANG
权利人:HARVARD COLLEGE
摘要:The present invention provides desosamine and mycaminose analogs and nitro sugars and methods for their preparation. The invention also provides methods of cyclizing a compound of Formula (A′) with glyoxal to give a nitro sugar of Formula (B). Methods for the preparation of compound of Formula (D′) are provided comprising cyclization of a nitro alcohol to give a nitro sugar and reduction and alkylation of the nitro sugar to give a desosamine, mycaminose, or an analog thereof.

专利号:US-7579167-B2
优先权日:2002-10-08
标题:Polypeptides involved in the biosynthesis of spiramycins, nucleotide sequences encoding these polypeptides and applications thereof
发明人：BLONDELET-ROUAULT MARIE-HELENE; DOMINGUEZ HELENE; DARBON-RONGERE EMMANUELLE; GERBAUD CLAUDE; GONDRAN ANNE; KARRAY FATMA; LACROIX PATRICIA; OESTREICHER-MERMET-BOUVIER NATHALIE; JEAN-LUC PERNODET; TUPHILE KARINE
权利人:AVENTIS PHARMA S; CENTRE NAT RECH SCIENT
摘要:The present invention relates to the isolation and identification of novel genes of the biosynthetic pathway for spiramycins and to novel polypeptides involved in this biosynthesis. The invention also relates to a method for producing these polypeptides. It also relates to the use of these genes for the purpose of increasing the levels of production and the purity of the spiramycin produced. The invention relates in particular to a microorganism which produces spiramycin I but which does not produce spiramycin II and III, and to the use of such a microorganism. The invention also relates to the use of the genes of the biosynthetic pathway for spiramycins for constructing mutants which can lead to the synthesis of novel antibiotics or to derived forms of spiramycins. The invention also relates to the molecules produced through the expression of these genes and to pharmacologically active compositions of a molecule produced through the expression of such genes.

专利号:US-2002045220-A1
优先权日:1999-10-13
标题:Biosynthesis of polyketide synthase substrates
发明人：KHOSLA CHAITAN; PFEIFER BLAINE
摘要:The use of enzymes which catalyze the production of starter and extender units for polyketides is described. In addition, modified loading modules are described, which can accept a variety of starting units such as substituted benzoates, and which can be used to generate substituted derivatives of natural products. These enzymes may be used to enhance the yield of polyketides that are natively produced or polyketides that are rationally designed. By using these techniques, the synthesis of a complete polyketide has been achieved in E. col. This achievement permits a host organism with desirable characteristics to be used in the production of such polyketides and to assess the results of gene shuffling.