专利号:EP-3835306-A1 优先权日:2019-12-13 标题:Microwave-assisted method for synthesis of oligo- and polysaccharides on solid phase 权利人:MAX PLANCK GESELLSCHAFT 摘要:The present invention relates to a method for synthesizing oligo- and polysaccharides using microwave radiation, in particular to a method and a device for automated synthesis of oligo- and polysaccharides.
专利号:US-2024117398-A1 优先权日:2021-01-20 标 题:Production of oligosaccharides comprising ln3 as core structure in host cells 发明人:AESAERT SOFIE; BEAUPREZ JOERI; PETERS GERT; VERCAUTEREN ANNELIES 权利人:INBIOSE NV 摘要:Described is a method of producing an oligosaccharide comprising a lacto-N-triose (LN3; GlcNAc-beta1,3-Gal-beta1,4-Glc) as a core trisaccharide by cultivation with a genetically modified cell, as well as the genetically modified cell used in the method. The genetically modified cell comprises at least one nucleic acid sequence coding for a galactoside beta-1,3-N-acetylglucosaminyltransferase and a glycosyltransferase involved in the synthesis of an oligosaccharide comprising LN3 as a core trisaccharide and at least one nucleic acid sequence expressing a membrane protein. Furthermore, the present invention provides for a purification of the oligosaccharide comprising LN3 as a core trisaccharide from the cultivation.
专利号:US-2024191270-A1 优先权日:2021-04-16 标 题 :Fermentative production 发明人:BEAUPREZ JOERI; COUSSEMENT PIETER; DECOENE THOMAS 权利人:INBIOSE NV 摘要:The present invention is in the technical field of synthetic biology and metabolic engineering. More particularly, the present invention is in the technical field of metabolically engineered cells and use of said cell in a cultivation, preferably a fermentation. The present invention describes a cell for the production of a compound. The cell comprises a pathway for the production of the compound, which can be a disaccharide, oligosaccharide and/or a Neu(n)Ac-containing bioproduct, wherein (n) is 4, 5, 7, 8 or 9 or a combination thereof. The cell is metabolically engineered for enhanced synthesis of acetyl-Coenzyme A. The invention also resides in a method of producing such compound by cultivation, preferably a fermentation, with such a cell.
专利号:US-2022403431-A1 优先权日:2020-02-14 标 题 :Glycominimized bacterial host cells 发明人:BEAUPREZ JOERI; DE MAESENEIRE SOFIA; SNOECK NICO 权利人:INBIOSE NV 摘要:This disclosure is in the technical field of synthetic biology and metabolic engineering. The disclosure provides engineered viable bacteria having a reduced or abolished synthesis of poly-N-acetyl-glucosamine (PNAG), Enterobacterial Common Antigen (ECA), cellulose, colanic acid, core oligosaccharides, Osmoregulated Periplasmic Glucans and Glucosylglycerol (O), glycan, and trebalose. The disclosure further provides methods for the production of bioproduct by the viable bacteria and uses thereof. Furthermore, the disclosure is in the technical field of fermentation of metabolically engineered microorganisms producing bioproduct.
专利号:US-11772063-B2 优先权日:2018-12-18 标 题:Method and device for producing saccharides and saccharide arrays 发明人:LÖFFLER FELIX; MENDE MARCO; EICKELMANN STEPHAN; SEEBERGER PETER H; TSOUKA ALEXANDRA; HEIDEPRIEM JASMIN; PARIS GRIGORI 权利人:MAX PLANCK GESELLSCHAFT 摘要:The present invention relates to a method and a device for producing saccharides and saccharide arrays. Said method is particularly useful for the synthesis of saccharides in parallel and of high-density saccharide arrays, such as microarrays, which are required for high-throughput screenings.
专利号:EP-4045182-A1 优先权日:2019-11-04 标 题:Microwave-assisted method for synthesis of oligo- and polysaccharides on solid phase
参考文献:10.1016/j.ijbiomac.2025.142338 摘要:Loera-Rubalcava J, García-Maldonado E, Rodríguez-Romero A, Quintero-Martínez A, Macías-Rubalcava ML, Cano-Sánchez P, Ramírez-Rodríguez MA, Espinosa-Pérez G, Hernández-Santoyo A. A mytilectin from Mytilus californianus: Study of its unique galactoside interactions, oligomerization patterns, and antifungal activity. International Journal of Biological Macromolecules. 2025 May;308():142338. doi: 10.1016/j.ijbiomac.2025.142338.