专利号:US-2025147014-A1 优先权日:2022-02-14 标题:Plasmon-enhanced fluorescence biochemical sensors 发明人:PINCHUK ANATOLI; SERGEYEVA TETYANA; YARYNKA DARIA; CHEGEL VLADIMIR; LOPATYNSKYI ANDRI 权利人:UNIV COLORADO REGENTS; THE REGENTS OF THE UNIV OF COLORADO A BODY 摘要:A highly sensitive technology enabling selective recognition of aflatoxins such as in contaminated cereal samples. Methods for the synthesis of AgNP-containing MIP membranes from the monomer mixtures containing both methacrylate monomers and oligomers as well as AgNOs solution in dimethylformamide enable the detection system. The AgNP were formed in the structure of the polymeric membranes during the pre-heating step followed by the UV-initiated polymerization. The highly selective sensor elements where MIP membranes are combined with the Ag nanostructures as signal amplifiers (LSPR-MIP nanochips) can be stored separately and used with a simple optical recognition system as selective elements of point-of-care sensor devices, even in in-field conditions. Composite plasmonic membranes provide both highly selective recognition of the target toxin and the generation of an enhanced optical signal, allowing aflatoxin B1 detection at ultralow concentrations.
专利号:US-10287592-B2 优先权日:2014-02-07 标 题:Bacillus host 发明人:VAN RIJ EVERT TJEERD; SPAANS MARTINE; HILLEBRAND HENDRIKUS BERNARDUS CAROLUS 权利人:DSM IP ASSETS BV 摘要:The invention provides Bacillus host cell deficient in the production of a neutral protease and of a alkaline protease which host cell comprises a nucleic acid construct, wherein the nucleic acid construct comprises a polynucleotide encoding a compound of interest or encoding a compound involved in the synthesis of a compound of interest operably linked to a promoter sequence allowing for expression of the polynucleotide in the host cell, wherein the promoter comprises a bacteriophage promoter sequence, more preferably a bacteriophage SPO1 promoter sequence. Said host cell can advantageously be used in a method of production of a compound of interest.
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合成参考文献
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