CAS: 54375-47-2; Calcein Blue

该化合物是生物和化学应用中常用的一种荧光染色,其特征是亮蓝色荧光,它能用于各种成像技术,特别是显微镜;该化合物在水中溶解,并显示出高量产量,从而可以对生物样本进行有效的可视化; Calcein blue经常被用作细胞可行性研究的追踪器,因为它能够渗透活细胞并释放荧光,表明代谢活动; 它在生理条件下的稳定性和与各种生物系统的兼容性使它成为研究的宝贵工具; 此外, Calceen blue可以与其他荧光标记结合使用,使多色成像成为可能. 但是,与许多荧光染一样,它可能具有光性和潜在的细胞毒性,在设计实验时应该加以考虑. 总的来说, Calcein blue作为生物,细胞生物学和环境监测领域的重要试剂.

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    📜,钙黄绿素,二甲酚橙 反应生成 钙黄绿素蓝
    参考文献:Fluorescently Labeled Calcium Phosphate Surfaces
    标题:Fluorescently Labeled Calcium Phosphate Surfaces
    摘要:本文描述了一种由基底涂覆有荧光标记的磷酸钙涂层的基板.这些基板在培养和研究各种细胞的活动方面非常有用.这些基板可用于培养细胞的溶液和图像分析.还公开了生产和使用这种涂层基板的新方法.

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    专利号:US-9732112-B2
    优先权日:2010-05-28
    标 题:Synthesis of 2′,3′-dideoxynucleosides for automated DNA synthesis and pyrophosphorolysis activated polymerization
    发明人:MA ZHAOCHUN; MULLAH KHAIRUZZAMAN BASHAR; EASON ROBERT G
    权利人:LIFE TECHNOLOGIES CORP
    摘要:Methods for preparation of 2′,3′-dideoxynucleotides support structures, such as 2′,3′-dideoxyguanosine, 2′,3′-dideoxyadenosine, and 3′-deoxythymidine support structures are disclosed. Various methods of using such structures are also provided, such as their use for automated DNA synthesis and pyrophosphorolysis activated polymerization.

    专利号:WO-2025007089-A1
    优先权日:2023-06-30
    标 题:Compositions, methods and kits for nucleic acids synthesis and amplification
    发明人:BERKMAN JENNIFER; URBONAS IGNAS; ABREU DANIELA CRUZ; LIU CHENGJING; YEN CHIA AN; ONG KIMBERLIE SOOHOO; WEI WEI; GOMEZ SILVAN MARIA CINTA
    权利人:LIFE TECHNOLOGIES CORP; THERMO FISHER SCIENTIFIC BALTICS UAB
    摘要:The present disclosure is directed to compositions, methods and kits useful for the synthesis of nucleic acid molecules. More specifically, compositions, methods and kits are provided for the amplification of nucleic acid molecules in an one-step qPCR or RT-qPCR procedure comprising a reverse transcriptase, a DNA polymerase, at least one dNTP, and at least one stabilizing agent, wherein said at least one stabilizing agent increases stability of an assembled polymerase chain reaction.

    专利号:US-9005895-B2
    优先权日:2010-06-21
    标题:Compositions, methods and kits for nucleic acid synthesis and amplification
    发明人:WOO CORA L; BERKMAN JENNIFER; YIM PRISCILLA W
    权利人:WOO CORA L; BERKMAN JENNIFER; YIM PRISCILLA W; LIFE TECHNOLOGIES CORP
    摘要:The present invention is directed to compositions, methods and kits useful for the synthesis of nucleic acid molecules. More specifically, compositions, methods and kits are provided for the amplification of nucleic acid molecules in a one-step RT-PCR procedure comprising one or more agents used to increase tolerance to PCR inhibitors.

    专利号:US-2004110228-A1
    优先权日:2002-04-01
    标 题:Combinatorial organic synthesis of unique biologically active compounds
    发明人:MCALPINE SHELLI R; TAYLOR RACHEL E; BOLLA MEGAN L; SEGALL ANCA M
    摘要:The invention provides a method for making a combinatorial library of cyclic compounds, such as Holliday junction-trapping compounds, comprising the steps of (a) obtaining a plurality of trimers according to the generic structure X 1- X 2- X 3 , wherein X 1 , X 2 and X 3 can be independently any naturally or nonnaturally occurring amino acids or peptidomimetics thereof; (b) optionally coupling a spacer S to the trimer at either end; (c) cyclizing two trimers or trimer-spacer conjugates in a head-to-tail orientation; thereby obtaining a combinatorial library of compounds, wherein the library does not include an unmodifed or naturally occurring Holliday Junction-trapping compounds. The invention additionally provides methods macrocyclic compounds that are synergimycin derivatives.

    专利号:US-8658776-B2
    优先权日:2010-05-28
    标题:Synthesis of 2′,3′-dideoxynucleosides for automated DNA synthesis and pyrophosphorolysis activated polymerization

    专利号:US-2011294122-A1
    优先权日:2010-05-28
    标 题:Synthesis of 2', 3'-Dideoxynucleosides for Automated DNA Synthesis and Pyrophosphorolysis Activated Polymerization

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    参考文献:10.1007/s00774-011-0291-7
    摘要:Burt-Pichat B, Follet H, Toulemonde G, Arlot M, Delmas P, Chapurlat R. Methodological approach for the detection of both microdamage and fluorochrome labels in ewe bone and human trabecular bone. Journal of Bone and Mineral Metabolism. 2011 Jul 13;29(6):756–64. doi: 10.1007/s00774-011-0291-7.
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