专利号:US-9732112-B2 优先权日:2010-05-28 标 题:Synthesis of 2′,3′-dideoxynucleosides for automated DNA synthesis and pyrophosphorolysis activated polymerization 发明人:MA ZHAOCHUN; MULLAH KHAIRUZZAMAN BASHAR; EASON ROBERT G 权利人:LIFE TECHNOLOGIES CORP 摘要:Methods for preparation of 2′,3′-dideoxynucleotides support structures, such as 2′,3′-dideoxyguanosine, 2′,3′-dideoxyadenosine, and 3′-deoxythymidine support structures are disclosed. Various methods of using such structures are also provided, such as their use for automated DNA synthesis and pyrophosphorolysis activated polymerization.
专利号:WO-2025007089-A1 优先权日:2023-06-30 标 题:Compositions, methods and kits for nucleic acids synthesis and amplification 发明人:BERKMAN JENNIFER; URBONAS IGNAS; ABREU DANIELA CRUZ; LIU CHENGJING; YEN CHIA AN; ONG KIMBERLIE SOOHOO; WEI WEI; GOMEZ SILVAN MARIA CINTA 权利人:LIFE TECHNOLOGIES CORP; THERMO FISHER SCIENTIFIC BALTICS UAB 摘要:The present disclosure is directed to compositions, methods and kits useful for the synthesis of nucleic acid molecules. More specifically, compositions, methods and kits are provided for the amplification of nucleic acid molecules in an one-step qPCR or RT-qPCR procedure comprising a reverse transcriptase, a DNA polymerase, at least one dNTP, and at least one stabilizing agent, wherein said at least one stabilizing agent increases stability of an assembled polymerase chain reaction.
专利号:US-9005895-B2 优先权日:2010-06-21 标题:Compositions, methods and kits for nucleic acid synthesis and amplification 发明人:WOO CORA L; BERKMAN JENNIFER; YIM PRISCILLA W 权利人:WOO CORA L; BERKMAN JENNIFER; YIM PRISCILLA W; LIFE TECHNOLOGIES CORP 摘要:The present invention is directed to compositions, methods and kits useful for the synthesis of nucleic acid molecules. More specifically, compositions, methods and kits are provided for the amplification of nucleic acid molecules in a one-step RT-PCR procedure comprising one or more agents used to increase tolerance to PCR inhibitors.
专利号:US-2004110228-A1 优先权日:2002-04-01 标 题:Combinatorial organic synthesis of unique biologically active compounds 发明人:MCALPINE SHELLI R; TAYLOR RACHEL E; BOLLA MEGAN L; SEGALL ANCA M 摘要:The invention provides a method for making a combinatorial library of cyclic compounds, such as Holliday junction-trapping compounds, comprising the steps of (a) obtaining a plurality of trimers according to the generic structure X 1- X 2- X 3 , wherein X 1 , X 2 and X 3 can be independently any naturally or nonnaturally occurring amino acids or peptidomimetics thereof; (b) optionally coupling a spacer S to the trimer at either end; (c) cyclizing two trimers or trimer-spacer conjugates in a head-to-tail orientation; thereby obtaining a combinatorial library of compounds, wherein the library does not include an unmodifed or naturally occurring Holliday Junction-trapping compounds. The invention additionally provides methods macrocyclic compounds that are synergimycin derivatives.
专利号:US-8658776-B2 优先权日:2010-05-28 标题:Synthesis of 2′,3′-dideoxynucleosides for automated DNA synthesis and pyrophosphorolysis activated polymerization
专利号:US-2011294122-A1 优先权日:2010-05-28 标 题:Synthesis of 2', 3'-Dideoxynucleosides for Automated DNA Synthesis and Pyrophosphorolysis Activated Polymerization
参考文献:10.1007/s00774-011-0291-7 摘要:Burt-Pichat B, Follet H, Toulemonde G, Arlot M, Delmas P, Chapurlat R. Methodological approach for the detection of both microdamage and fluorochrome labels in ewe bone and human trabecular bone. Journal of Bone and Mineral Metabolism. 2011 Jul 13;29(6):756–64. doi: 10.1007/s00774-011-0291-7.