专利号:US-2007174933-A1 优先权日:2006-01-25 标题 :Altering levels of anti-nutrient factors in plants 发明人:HANNOUFA ABDELALI; SCHAFER ULRIKE A 权利人:HANNOUFA ABDELALI; SCHAFER ULRIKE A 摘要:A method for reducing the level of one or more than one protein in a plant or a tissue within the plant, and plants with reduced levels of target proteins are provided. The method involves introducing a nucleic acid sequence into the plant, where the nucleic acid sequence comprises a regulatory region operatively associated with a silencing nucleotide sequence. The expression of the silencing nucleotide sequence reduces or eliminates the expression of two or more than two enzymes involved in the synthesis of the one or more than one protein. The reduced level of the one or more than one protein may be determine by comparing the level of the one or more than one protein in the plant, or a tissue of the plant, with a level of the one ore more than one protein in a second plant, or the tissue from the second plant, that does not express the nucleic acid sequence. Plants and seeds obtained from plants with reduced levels of target proteins, and reduced anti-nutrient factors are also provided
专利号:US-2022228171-A1 优先权日:2019-07-17 标 题 :Compositions and production of nicked closed-ended dna vectors 发明人:ALKAN OZAN; KOTIN ROBERT MICHAEL; KERR DOUGLAS ANTHONY; MONDS RUSSELL; PELLETIER CAROLYN; STANTON MATTHEW 权利人:GENERATION BIO CO 摘要:The present application discloses methods for synthetic production and cell-free synthesis of DNA vectors, particularly closed-ended linear DNA vectors having one or more gaps (e.g., nicked ceDNA vectors, “neDNAâ€?) and adenoassociated-virus (AAV) vector which is single strand DNA having linear and continuous structure, for delivery and expression of a transgene in the host cell. The present invention also relates to an in vitro process for production of closed-ended DNA vectors, corresponding DNA vector products produced by the methods and uses thereof, and oligonucleotides and kits useful in the process of the present invention.
专利号:CN-106467913-A 优先权日:2015-08-18 标 题 :Probe inversion method for in situ synthesis of probe arrays
专利号:US-8871998-B2 优先权日:2009-04-24 标题:Method for producing chrysanthemum plant having petals containing modified anthocyanin 发明人:NODA NAONOBU; AIDA RYUTARO; SATO SANAE; OHMIYA AKEMI; TANAKA YOSHIKAZU 权利人:NODA NAONOBU; AIDA RYUTARO; SATO SANAE; OHMIYA AKEMI; TANAKA YOSHIKAZU; SUNTORY HOLDINGS LTD 摘要:Disclosed are a method for controlling flavonoid synthesis in a chrysanthemum plant or non- chrysanthemum plant by genetic recombination technology using a transcriptional regulatory region useful for altering flower color, a method for modifying anthocyanins, a method for producing a chrysanthemum plant or non- chrysanthemum plant containing modified anthocyanins in the petals thereof, and a chrysanthemum plant or non- chrysanthemum plant, progeny thereof, or vegetatively propagated products, part or tissue thereof transformed with the regulatory region. In the method according to the present invention, an expression vector or expression cassette containing a transcriptional regulatory region of perilla anthocyanin 3-acyltransferase gene, such as a nucleic acid containing the nucleotide sequence indicated in SEQ ID NO. 1, or a transcriptional regulatory region of pansy F3′5′H gene, such as the nucleotide sequence indicated in SEQ ID NO. 15, is used.
专利号:EP-1144653-B1 优先权日:1999-09-28 标题 :Thermostable enzyme promoting the fidelity of thermostable dna polymerases- for improvement of nucleic acid synthesis and amplification in vitro
专利号:WO-2024049312-A2 优先权日:2022-09-02 标 题:A method for the synthesis and purification of a nucleotide and/or a nucleoside, a modified nucleotide and/or a nucleoside, a dna molecule containing a single- or double-stranded oligonucleotide chain, an oligonucleotide library, use of the oligonucleotide library
1: Nazifi M, Oryan S, Eslimi Esfahani D, Moghadamnia AA, Ashrafpour M. Chronic Administration of Piperine Attenuates Behavioural, Biochemical and Histological Alterations induced by Intracerebroventricular Streptozotocin in Rats. Basic Clin Pharmacol Toxicol. 2018 Jun 11. doi: 10.1111/bcpt.13052. [Epub ahead of print] doi: 10.1016/j.jnutbio.2018.04.010. [Epub ahead of print] doi: 10.1016/j.jconrel.2018.05.011. [Epub ahead of print] Review. doi: 10.1186/s12870-018-1302-9. 9: Ding Y, Wang C, Wang Y, Xu Y, Zhao J, Gao M, Ding Y, Peng J, Li L. Development and evaluation of a novel drug delivery: Soluplus(®)/TPGS mixed micelles loaded with piperine in vitro and in vivo. Drug Dev Ind Pharm. 2018 May 27:1-8. doi: 10.1080/03639045.2018.1472277. [Epub ahead of print] pii: BSR20180503. doi: 10.1042/BSR20180503. Print 2018 Jun 29. doi: 10.1016/j.biopha.2018.04.108. Epub 2018 Apr 24. doi: 10.1002/mnfr.201800086. Epub 2018 May 17. doi: 10.3389/fphar.2018.00244. eCollection 2018.
合成参考文献
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