专利号:US-2012190064-A1 优先权日:2004-10-01 标题 :Feeding buffers, systems, and methods for in vitro synthesis of biomolecules 发明人:KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA 权利人:KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA; LIFE TECHNOLOGIES CORP 摘要:Compositions, methods and kits for in vitro systems for synthesis of biomolecules such as polypeptides, are provided herein. Cell extracts that provide enhanced yields of soluble proteins using in vitro protein synthesis methods are provided. The invention also includes methods for producing high yields of proteins by the addition of a feeding solution that includes amino acids and an energy source to an ongoing in vitro synthesis system. The invention also includes methods of using a high-yield in vitro synthesis system to produce large quantities of proteins with incorporated labeled amino acids for analysis by methods such as by NMR. The invention further includes vectors for enhanced production of proteins from nucleic acid templates using in vitro synthesis systems.
专利号:WO-9616071-A1 优先权日:1994-11-21 标题:PROCESSES FOR THE SYNTHESIS OF 3'-SUBSTITUTED LEWISx COMPOUNDS 发明人:SRIVASTAVA OM; IPPOLITO ROBERT; SRIVASTAVA GEETA; SZWEDA ROMAN; OHUCHI TOSHIO 权利人:GLYCOMED INC; SRIVASTAVA OM; IPPOLITO ROBERT; SRIVASTAVA GEETA; SZWEDA ROMAN; OHUCHI TOSHIO 摘要:Disclosed are processes for the chemical synthesis of 3'-substituted Lewis-OR compounds where R is an aglycon of at least one carbon atom. One particular compound prepared by the processes of this invention is 8-methoxycarbonyl-octyl-2-acetamido-3-O-( alpha -L-fucopyranosyl)-4-O-[3-O-sulfo- beta -D-galactopyranosyl]-2-deoxy- beta -D-glucopyranoside.
专利号:US-2002037834-A1 优先权日:2000-09-08 标题 :Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis 发明人:ASTATKE MEKBIB; GEBEYEHU GULILAT 权利人:INVITROGEN CORP 摘要:The present invention relates to cationic and polycationic compositions and methods for enhancing synthesis of nucleic acid molecules. In a preferred aspect, the invention relates to inhibition or control of nucleic acid synthesis, sequencing or amplification. Specifically, the present invention discloses cationic and polycationic molecules, compounds, and compositions having affinity for double-stranded and/or single-stranded nucleic acid molecules and/or single-stranded/double-stranded nucleic acid complexes (e.g., primer/template complexes, double-stranded templates, single-stranded templates or single-stranded primers) for use in such enhanced synthesis. The cationic and polycationic molecules, compounds, and compositions of the invention are capable of inhibiting nonspecific nucleic acid synthesis at ambient temperature. Thus, in a preferred aspect, the invention relates to “hot startâ€? synthesis of nucleic acid molecules. Accordingly, the invention prevents non-specific nucleic acid synthesis at low temperatures, for example during reaction set up. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the cationic and polycationic molecules, compounds, and compositions of the invention. The invention also relates to compositions prepared for carrying out the methods of the invention and to compositions made after or during such methods. The invention also generally relates to compositions useful for inhibiting or preventing degradation of various nucleic acid molcules.
专利号:US-9932582-B2 优先权日:2014-01-14 标题 :Materials and methods for modulation of tendon healing 发明人:GILCHRIST DEREK STEWART; MILLAR NEAL LINDSAY 权利人:UNIV GLASGOW COURT 摘要:The invention relates to the use of microRNA 29 and precursors and mimics thereof for the modulation of tendon injury and the biomechanical properties of tendon. In particular, the invention derives from the finding that synthesis of type 1 collagen in tenocytes is less sensitive to miR-29 than is synthesis of type 3 collagen, thus enabling the balance between the collagen subtypes to be modulated in favor of type 1 collagen, mitigating reduction in biomechanical properties during healing.
专利号:US-2014378538-A1 优先权日:2011-12-14 标 题:Methods of responding to a biothreat 发明人:BANCEL STEPHANE 权利人:MODERMA THERAPEUTICS INC 摘要:The present disclosure provides for devices, in particular mobile devices, which may be used in the synthesis of modified nucleic acid molecules, in particular modified mRNA molecules. The device for making the modified nucleosides, modified nucleotides and modified nucleic acids (e.g., mRNA) disclosed herein may be mobile devices comprising at least one sample block for insertion of one or more sample vessels, a device base with electronic control units for the sample block, a voltage supply, and one or more reagent(s) for the synthesis of at least one nucleic acid.
专利号:US-9254327-B2 优先权日:2010-05-10 标 题 :Methods and compositions for delivery of active agents 发明人:MANOHARAN MUTHIAH; RAJEEV KALLANTHOTTATHIL G 权利人:MANOHARAN MUTHIAH; RAJEEV KALLANTHOTTATHIL G; ALNYLAM PHARMACEUTICALS INC 摘要:A lipid particle can include a cationic lipid. Synthesis of the cationic lipid can include a ylide-based reaction, such as a Wittig reaction or sulfur ylide reaction. In some cases, the synthesis can also include a Michael addition or a related addition reaction.
1: Iwasaki F, Suga K, Okamoto Y, Umakoshi H. Characterization of DDAB/Cholesterol Vesicles and Its Comparison with Lipid/Cholesterol Vesicles. J Nanosci Nanotechnol. 2018 Mar 1;18(3):1989-1994. doi: 10.1166/jnn.2018.14252. 172(3):962-74. doi: 10.1016/j.jconrel.2013.09.015. Epub 2013 Sep 25. 3: Kusumoto K, Ishikawa T. Didodecyldimethylammonium bromide (DDAB) induces caspase-mediated apoptosis in human leukemia HL-60 cells. J Control Release. 2010 Oct 15;147(2):246-52. doi: 10.1016/j.jconrel.2010.07.114. Epub 2010 Jul 30. 19(2):131-40. doi: 10.1080/08982100802642457. 127(46):16224-32. doi: 10.1021/ja0546572. 167:337-344. doi: 10.1016/j.colsurfb.2018.04.031. Epub 2018 Apr 17. 26(7):5243-53. doi: 10.1021/la903387n. 132(20):7025-37. doi: 10.1021/ja909514j.
合成参考文献
参考文献:10.1007/bf02722997 摘要:Søndergaard L. Efficiency of different lipofection agents in drosophila S-2 cells. In Vitro Cellular & Developmental Biology - Animal. 1996 Jul;32(7):386–7. doi: 10.1007/bf02722997. 参考文献:10.1007/978-1-59745-210-6_9 摘要:Dass CR. Drug delivery in cancer using liposomes. Methods Mol Biol. 2008;437():177–82. doi: 10.1007/978-1-59745-210-6_9. 参考文献:10.1007/s12192-012-0397-4 摘要:Spiering R, van der Zee R, Wagenaar J, van Eden W, Broere F. Mycobacterial and mouse HSP70 have immuno-modulatory effects on dendritic cells. Cell Stress and Chaperones. 2012 Dec 27;18(4):439–46. doi: 10.1007/s12192-012-0397-4. 参考文献:10.1007/s10661-014-3824-4 摘要:Jeong S, Kim H. Filtration of fullerene and copper oxide nanoparticles using surface-modified microfilters. Environmental Monitoring and Assessment. 2014 May 23;186(9):5855–64. doi: 10.1007/s10661-014-3824-4. 参考文献:10.2116/analsci.31.979 摘要:Ohno M, Toyota T, Nomoto T, Fujinami M. Changes in Interfacial Tension of a Lipid Membrane Formed at the Water/Chloroform Interface upon DNA Complex Formation. Analytical Sciences. 2015 Oct 10;31(10):979–86. doi: 10.2116/analsci.31.979.