CAS: 1323-19-9; Tips

📜在 哌啶,N-甲基吡咯烷酮体系中,化学反应 0.33H,反应生成三(1-甲基乙基)萘磺酸钠
参考文献:Site-Specific N-And C-Terminal Labeling Of A Single Polypeptide Using Sortases Of Different Specificity
标题:Site-Specific N-And C-Terminal Labeling Of A Single Polypeptide Using Sortases Of Different Specificity
摘要:The Unique Reactivity Of Two Sortase Enzymes,Srta(Staph) From Staphylococcus Aureus And Srta(Strep) From Streptococcus Pyogenes,Is Exploited For Site-Specific Labeling Of A Single Polypeptide With Different Labels At Its N And C Termini. Srta(Strep) Is Used To Label The Protein'S C Terminus At An Lpxtg Site With A Fluorescently Labeled Dialanine Nucleophile. Selective N-Terminal. Labeling Of Proteins Containing N-Terminal Glycine Residues Is Achieved Using Srta(Staph) And Lpxt Derivatives. The Generality Of N-Terminal Labeling With Srta(Staph) Is Demonstrated By Near-Quantitative Labeling Of Multiple Protein Substrates With Excellent Site Specificity.
Doi:10.1021/ja902681K