专利号:EP-1354960-A1 优先权日:2002-04-08 标 题 :Assay for synthesis activity of hydrolases 发明人:BORNSCHEUER UWE T PROF DR; KANARZYCKA MONIKA; HILLS GEOFFREY DR 权利人:DEGUSSA 摘要:The present invention provides an assay for testingnhydrolase enzymes, in particular esterases or lipases, forntheir ability to catalyse synthesis reactions in nonaqueous,norganic media. The assay can serve as a tool fornhigh-throughput screening of libraries of enzymes stemmingne.g. from directed evolution processes. n The assay comprises an enzymatically catalysedntransesterification reaction releasing a carbonyl compoundnindirectly measured by its reaction with a further moleculenthus rendering it detectable.
专利号:CN-110658163-A 优先权日:2018-06-29 标 题 :A method for reaction monitoring in the synthesis of DNA-encoded compounds
专利号:EP-1359225-A1 优先权日:2002-04-08 标题:Assay for synthesis activity of hydrolases
专利号:EP-1359225-B1 优先权日:2002-04-08 标题 :Assay for synthesis activity of hydrolases 发明人:BORNSCHEUER UWE T PROF DR; KANARZYCKA MONIKA; HILLS GEOFFREY DR 权利人:DEGUSSA
专利号:DE-60309620-T2 优先权日:2002-04-08 标 题:Method for determining the synthesis activity of hydrolases
[参考文献]: G. Gübitz, Et Al. Fluorogenic Labelling Of Carbonylcompounds With 7-Hydrazine-4-Nitrobenzo-2-Oxa-1,3-Diazole (Nbd-H). Journal Of Liquid Chromatography. 13 Dec 2006, 7(4): 839-854 [参考文献]: Gerlach, J., Et Al. 2-D Solid-State Assay Platform: A Tool For Screening Aldehyde-Releasing Enzyme Activity In Colonies. Microchim Acta 156, 209-218 (2006).