CAS: 10048-13-2; Sterigmatocystin

📜(3Ar,12Cs)-6,8-二羟基-3A,12C-二氢-7H-呋喃并[3',2':4,5]呋喃并[2,3-C]氧杂蒽-7-酮,S-腺苷蛋氨酸 反应生成氢(+1)阳离子,S-(5'-腺苷)-L-高半胱氨酸,柄曲菌素
参考文献:Cloning And Characterization Of The O-Methyltransferase I Gene ( Dmta ) From Aspergillus Parasiticus Associated With The Conversions Of Demethylsterigmatocystin To Sterigmatocystin And Dihydrodemethylsterigmatocystin To Dihydrosterigmatocystin In Aflatoxin Biosynthesis
标题:Cloning And Characterization Of The O-Methyltransferase I Gene ( Dmta ) From Aspergillus Parasiticus Associated With The Conversions Of Demethylsterigmatocystin To Sterigmatocystin And Dihydrodemethylsterigmatocystin To Dihydrosterigmatocystin In Aflatoxin Biosynthesis
摘要:摘要 O-甲基转移酶 I 在黄曲霉毒素的生物合成过程中催化去甲基甾基麦角甾胱氨酸向甾基麦角甾胱氨酸的转化以及二氢去甲基甾基麦角甾胱氨酸向二氢甾基麦角甾胱氨酸的转化.在这项研究中,通过基因组克隆和 Cdna 克隆,对黄曲霉毒素生物合成过程中编码 O-甲基转移酶 I 的基因的基因组克隆和 Cdna 克隆都是通过 Pcr 策略完成的,如传统的基于 N-等方法完成了基因组克隆和编码 O-甲基转移酶 I 基因的 Cdna 克隆. 寄生曲霉 Niah-26进行基因测序.基因组序列与 Dmta 的 Cdna 进行了比较. Dmta 基因组序列与 Dmta 的 Cdna 进行比较后发现,编码区被三个短内含子打断.Dmta 的 Cdna Dmta 基因的 Cdna 长 1,373 Bp,编码一种 386 氨基酸的蛋白质,推导分子量为 43,023,这与十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定的蛋白质分子量一致.推导出的蛋白质的 C 端半部分显示出 76.3%的同一性,与 黑曲霉 Stcp 蛋白的编码区显示出 76.3% 的同一性,而 Dmta 的 5'侧翼区域有 73.0%的相同性. 侧翼区域 基因的 5'侧翼区有 73.0%的同一性,这表明 Stcp 基因的翻译可能是从蛋氨酸上游的一个位点开始的,该位点不同于先前提出的位点.此外,对 Dmta 基因的 5'和 3'侧翼区进行的研究也表明 Dmta 基因的 5'和 3'侧翼区进行了检测,结果表明 Dmta 基因位于黄曲霉毒素生物合成簇中,介于 和 和 Ord-2 基因之间(并与 Ord-2Northern 印迹显示 Dmta 基因的表达受培养基成分和培养温度的影响,其表达模式与黄曲霉毒素基因簇中其他基因的表达模式相关.此外,Southern 印迹和 Pcr 分析显示 Dmta 基因的 Southern 印迹和 Pcr 分析表明 Dmta 同源物存在于 曲霉 Sys-2.
Doi:10.1128/aem.65.11.4987-4994.1999

专利信息

专利号:US-7510852-B2
优先权日:2002-10-18
标题:Biosynthetic genes and host cells for the synthesis of polyketide antibiotics and method of use
发明人：ROYER MONIQUE; GABRIEL DEAN W; FRUTOS ROGER; ROTT PHILIPPE
权利人:CIRAD; UNIV FLORIDA
摘要:Three gene clusters that together encode albicidin biosynthesis, the complete gene DNA sequences, and the deduced protein sequences for the enzymes and methods for using the DNA sequences are disclosed and discussed as well as methods for plant protection and creating new antibiotics. The novel Albicidin family of antibiotics is disclosed and their structure deduced.

专利号:US-7838053-B2
优先权日:2004-03-11
标题:Barley for production of flavor-stable beverage
发明人：BREDDAM KLAUS; OLSEN OLE; SKADHAUGE BIRGITTE; LOK FINN; KNUDSEN SOEREN; BECH LENE MOELSKOV
权利人:CARLSBERG AS
摘要:According to the invention, there is provided null-LOX-1 barley and plant products produced thereof, such as malt manufactured by using barley kernels defective in synthesis of the fatty acid-converting enzyme lipoxygenase-1. Said enzyme accounts for the principal activity related to conversion of linoleic acid into 9-hydroperoxy octadecadienoic acid, a lipoxygenase pathway metabolite, which-through further enzymatic or spontaneous reactions-may lead to the appearance of trans-2-nonenal. The invention enables brewers to produce a beer devoid of detectable trans-2-nonenal-specific off flavors, even after prolonged storage of the beverage.

专利号:US-6600029-B1
优先权日:1995-12-19
标题:Metabolic engineering of polyhydroxyalkanoate monomer synthases
发明人：SHERMAN DAVID H; WILLIAMS MARK D; XUE YONGQUAN
权利人:UNIV MINNESOTA
摘要:A novel pathway for the synthesis of polyhydroxyalkanoate is provided. A method of synthesizing a recombinant polyhydroxyalkanoate monomer synthase is also provided. These recombinant polyhydroxyalknoate synthases are derived from multifunctional fatty acid synthases or polyketide synthases and generate hydroxyacyl acids capable of polymerization by a polyhydroxyalknoate synthase.

专利号:US-2003194784-A1
优先权日:2001-04-17
标题:DNA encoding methymycin and pikromycin
发明人：SHERMAN DAVID H; LIU HUNG-WEN; XUE YONGQUAN; ZHAO LISHAN
摘要:A novel pathway for the synthesis of polyhydroxyalkanoates is provided. A method of synthesizing a recombinant polyhydroxyalkanoate monomer synthase is also provided. These recombinant polyhydroxyalkanoate synthases are derived from multifunctional fatty acid synthases or polyketide synthases and generate hydroxyacyl acids capable of polymerization by a polyhydroxyalkanoate synthase. Also provided is a biosynthetic gene cluster for methymycin and pikomycin as well as a biosynthetic gene cluster for desosamine.

专利号:US-6265202-B1
优先权日:1998-06-26
标 题 :DNA encoding methymycin and pikromycin
发明人：SHERMAN DAVID H; LIU HUNG-WEN; XUE YONGQUAN; ZHAO LISHAN
权利人:UNIV MINNESOTA
摘要:A novel pathway for the synthesis of polyhydroxyalkanoates is provided. A method of synthesizing a recombinant polyhydroxyalkanoate monomer synthase is also provided. These recombinant polyhydroxyalkanoate synthases are derived from multifunctional fatty acid synthases or polyketide synthases and generate hydroxyacyl acids capable of polymerization by a polyhydroxyalkanoate synthase. Also provided is a biosynthetic gene cluster for methymycin and pikomycin as well as a biosynthetic gene cluster for desosamine.

专利号:US-2009011476-A1
优先权日:2005-08-19
标 题 :Gene cluster and method for the biosynthesis of terrequinone a
发明人：HOFFMEISTER DIRK; KELLER NANCY P
权利人:WISCONSIN ALUMNI RES FOUND
摘要:The present invention provides a novel gene cluster containing five genes (tdiA-E) involved in indole alkaloid synthesis. Disruption of tdiB, encoding an enzyme with prenyltransferase activity, transferring dimethylallylpyrophosphate to C-2 of an indole structure, eliminated the production of the antitumor compound terrequinone A, a metabolite not known from A. nidulans . The invention further provides a method for expressing terrequinone A in a host cell and isolating purified terrequinone A therefrom.