专利号:US-9005895-B2 优先权日:2010-06-21 标题:Compositions, methods and kits for nucleic acid synthesis and amplification 发明人:WOO CORA L; BERKMAN JENNIFER; YIM PRISCILLA W 权利人:WOO CORA L; BERKMAN JENNIFER; YIM PRISCILLA W; LIFE TECHNOLOGIES CORP 摘要:The present invention is directed to compositions, methods and kits useful for the synthesis of nucleic acid molecules. More specifically, compositions, methods and kits are provided for the amplification of nucleic acid molecules in a one-step RT-PCR procedure comprising one or more agents used to increase tolerance to PCR inhibitors.
专利号:US-8685671-B2 优先权日:2005-10-27 标题 :Process for producing recombinant glycoproteins by culturing a Hansenula polymorpha mutant strain 发明人:KANG HYUN AH; PARK JEONG SEOK; KIM MOO WOONG; KIM EUN JUNG; MOON HYE YUN; OH DOO BYOUNG; HEO JOO HYUNG; RHEE SANG KI 权利人:KANG HYUN AH; PARK JEONG SEOK; KIM MOO WOONG; KIM EUN JUNG; MOON HYE YUN; OH DOO BYOUNG; HEO JOO HYUNG; RHEE SANG KI; KOREA RES INST OF BIOSCIENCE 摘要:The present invention relates to a process for producing a human-type glycoprotein having reduced glycosylation by genetically manipulating an enzyme involved in glycosylation using a Hansenula polymorpha system. In detail, the present invention relates to a process for producing a human-type glycoprotein by identifying a dolichyl-phosphate-mannose dependent α-1,3-mannosyltransferase gene from H. polymorpha , constructing a H. polymorpha mutant strain producing a glycoprotein exhibiting reduced glycosylation by disrupting the identified gene, and subjecting the mutant strain to various genetic manipulations for the synthesis of human-type glycan.
专利号:US-2002064816-A1 优先权日:1999-12-16 标 题 :Moss genes from physcomitrella patens encoding proteins involved in the synthesis of carbohydrates 发明人:LERCHL JENS; RENZ ANDREAS; EHRHARDT THOMAS; REINDL ANDREAS; CIRPUS PETRA; BISCHOFF FRIEDRICH; FRANK MARKUS; FREUND ANNETTE; DUWENIG ELKE; SCHMIDT RALF-MICHAEL; RESKI RALF 摘要:Isolated nucleic acid molecules, designated CMRP nucleic acid molecules, which encode novel CMRPs from Physcomitrella patens are described. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing CMRP nucleic acid molecules, and host cells and organisms into which the expression vectors have been introduced. The invention still further provides isolated CMRPs, mutated CMRPs, fusion proteins, antigenic peptides and methods for the improvement of production of a desired compound from transformed cells based on genetic engineering of CMRP genes in this organism.
专利号:US-2022411860-A1 优先权日:2015-09-28 标题:Design and synthesis of novel disulfide linker based nucleotides as reversible terminators for dna sequencing by synthesis
专利号:US-2023160000-A1 优先权日:2015-09-28 标 题:Synthesis of novel disulfide linker based nucleotides as reversible terminators for dna sequencing by synthesis
专利号:US-2023028388-A1 优先权日:2015-09-28 标 题 :Design and synthesis of novel disulfide linker based nucleotides as reversible terminators for dna sequencing by synthesis
参考文献:10.1111/j.1439-0507.1993.tb00757.x 摘要:Rüchel R, Margraf S. Rapid microscopical diagnosis of deep-seated mycoses following maceration of fresh specimens and staining with optical brighteners. Mycoses. 1993 Jul;36(7-8):239–42. doi: 10.1111/j.1439-0507.1993.tb00757.x. 参考文献:10.1111/j.1439-0507.1993.tb00758.x 摘要:Chander J, Chakrabarti A, Sharma A, Saini JS, Panigarhi D. Evaluation of Calcofluor staining in the diagnosis of fungal corneal ulcer. Mycoses. 1993 Jul;36(7-8):243–5. doi: 10.1111/j.1439-0507.1993.tb00758.x.