CAS: 126695-58-7; 4-N3Pfp-Nhs Ester

主要参考文献

参考标题:Stereoselective Synthesis Of Light-Activatable Perfluorophenylazide-Conjugated Carbohydrates For Glycoarray Fabrication And Evaluation Of Structural Effects On Protein Binding By Spr Imaging
作者:Lingquan Deng,Oscar Norberg,Suji Uppalapati,Mingdi Yan,Olof Ramström
摘要:A Series Of Light-Activatable Perfluorophenylazide (Pfpa)-Conjugated Carbohydrate Structures Have Been Synthesized And Applied To Glycoarray Fabrication. The Glycoconjugates Were Structurally Varied With Respect To Anomeric Attachment, S-, And O-Linked Carbohydrates, Respectively, As Well As Linker Structure And Length. Efficient Stereoselective Synthetic Routes Were Developed, Leading To The Formation Of The Pfpa-Conjugated Structures In Good Yields Over Few Steps. The Use Of Glycosyl Thiols As Donors Proved Especially Efficient And Provided The Final Compounds In Up To 70% Total Yield With High Anomeric Purities. Pfpa-Based Photochemistry Was Subsequently Used To Generate Carbohydrate Arrays On A Polymeric Surface, And Surface Plasmon Resonance Imaging (Spri) Was Applied For Evaluation Of Carbohydrate-Protein Interactions Using The Plant Lectin Concanavalin A (Con A) As A Probe. The Results Indicate Better Performance And Equal Efficiency Of S- And O-Linked Structures With Intermediate Linker Length.

合成参考文献

参考文献:10.1055/s-0031-1290444
摘要:Marchand-Brynaert J, Welle A, Billard F. Tri- and Tetravalent Photoactivable Cross-Linking Agents. Synthesis. 2012 Jun 18;44(14):2249–54. doi: 10.1055/s-0031-1290444.