CAS: 131-99-7; ((2R,3S,4R,5R)-3,4-Dihydroxy-5-(6-Hydroxy-9H-Purin-9-yl)Tetrahydrofuran-2-yl)Methyl Dihydrogen Phosphate

上下游产品

鸟苷酸 5'-Guanosine Monophosphate 85-32-5
肌苷 Inosine 58-63-9
鸟苷 Guanosine 118-00-3
5'-腺嘌呤核苷酸 5'-Adenosine Monophosphate 61-19-8
N6-Methyl Amp 4229-50-9
5’-三磷酸腺苷 Atp 56-65-5
9-[(3Ar,4R,6R,6Ar)-6-[[tert-Butyl(Diphenyl)Silyl]Oxymethyl]-2-[4-(Hydroxymethyl)Phenyl]-3A,4,6,6A-Tetrahydrofuro[3,4-D][1,3]Dioxol-4-Yl]-1H-Purin-6-One

📜9-[(3Ar,4R,6R,6Ar)-6-[[tert-Butyl(Diphenyl)Silyl]Oxymethyl]-2-[4-(Hydroxymethyl)Phenyl]-3A,4,6,6A-Tetrahydrofuro[3,4-D][1,3]Dioxol-4-Yl]-1H-Purin-6-One置于三氟乙酸体系中,用 二氯甲烷 用作溶剂,化学反应 0.33H,反应生成5'-肌苷酸
参考文献:Aicar 5'-单磷酸酯 (Zmp) 及其 4-N-烷基衍生物的简便固相合成
标题:Aicar 5'-单磷酸酯 (Zmp) 及其 4-N-烷基衍生物的简便固相合成
摘要:我们在此报告了一种简便的固相合成 5-Amino-1-β-D-Ribofuranosylimidazole-4-Carboxamide-5'-Monophosphate (Zmp),一种嘌呤核苷酸的生物合成前体,以及它的一小部分4-N-烷基衍生物.5-Amino-1-β-D-Ribofuranosylimidazole-4-Carboxamide (Aicar) 非常困难,直接的化学磷酸化通过在 N-1-的 5'-位安装一个合适的,完全保护的磷酸基团来规避(2,4-二硝基苯基)-肌苷,在嘌呤降解之前通过 2',3'-位连接到固体支持物,导致 5-氨基-咪唑-4-甲酰胺部分.还报道了形成 Zmp 咪唑的合理反应机制.
Doi:10.1002/ejoc.200901271

专利信息

专利号:US-2025257380-A1
优先权日:2023-01-31
标 题:Method for template-free de novo synthesis of long-chain nucleic acid, and use thereof
发明人：XU CHENG; LIU YUANXIAO
权利人:SHANGHAI TYPING BIOSCIENCE CO LTD
摘要:The present application relates to the fields of molecular biology and biotechnology, and in particular to a method for template-free de novo synthesis of a long-chain nucleic acid, including the following steps: S1, synthesis of double-stranded oligonucleotides; and S2, combination and ligation of the double-stranded oligonucleotides to obtain a target long-chain nucleic acid. The present application achieves continuous synthesis from single nucleotides to long-chain nucleic acids by means of the combination of S1 and S2, and has the advantages of no need for templates, high accuracy, low complexity and low cost.

专利号:US-8314209-B2
优先权日:2007-12-12
标 题 :Lipid-assisted synthesis of polymer compounds and methods for their use
发明人：RAJAMANI SUDHA; OLASAGASTI FELIX; DEAMER DAVID W; BENNER SEICO
权利人:RAJAMANI SUDHA; OLASAGASTI FELIX; DEAMER DAVID W; BENNER SEICO; UNIV CALIFORNIA
摘要:The invention herein disclosed provides for methods for the synthesis of polymers from monomers. In particular the method provides for the synthesis of polynucleotides from mononucleotides in the absence of catalytic enzymes. The method comprises providing an aqueous solution having a plurality of phospholipid molecules and monomer molecules; subjecting the aqueous solution to fluctuating temperature conditions; subjecting the aqueous solution to fluctuating cycles of drying and hydrating conditions; subjecting the aqueous solution to fluctuating [H + ] conditions; the fluctuating conditions thereby allowing formation of a chemical bond between at least two monomers to create a polymer. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.

专利号:US-7122693-B2
优先权日:1988-04-11
标题 :Acetaldehyde acetal compounds, their synthesis, and uses thereof
发明人：BELLEAU EXECUTRIX OF ESTATE PI; DIXIT DILIP M; NGUYEN-BA NGHE
权利人:SHIRE BIOCHEM INC
摘要:The acetaldehyde compounds, 2-thiobenzoylacetaldehyde diethylacetal, 2-benzoyloxyacetaldehyde bis(2-methoxyethyl)acetal, 2-hydroxyacetaldehyde bis( 2-methoxyethyl)acetal, 2-thiobenzoylacetaldehyde bis(2-methoxy-ethyl)acetal, and 2-thioacetaldehyde bis(2-methoxyethyl)acetal, are useful as intermediates in the synthesis of substituted 1,3-oxathiolanes and substituted 1,3-dioxolanes.

专利号:US-7402418-B2
优先权日:2001-09-20
标 题:Genes participating in the synthesis of fatty acid having trans-11-,cis-13-conjugated double bond and utilization thereof
发明人：OSUMI MARI; MURASE JUNKO; IMAMURA JUN
权利人:PLANTECH RES INST; INC ADMIN AGENCY NARO
摘要:An object of the present invention is to clone a gene which is involved in synthesis of fatty acid having trans-11-, cis-13-conjugated double bonds from fatty acid having a double bone at position Δ12. The present invention provides a gene having any one of the following nucleotide sequences:n (A) a nucleotide sequence encoding an amino acid sequence shown in SEQ ID NO: 1 or 12; (B) a nucleotide sequence encoding an amino acid sequence comprising a deletion, addition or substitution of one or several amino acids with respect to the amino acid sequence shown in SEQ NO: 1 or 12, and having an ability of synthesizing fatty acid having trans-11-, cis-13-conjugated double bonds from fatty acid having a double bond at position Δ12; (C) a nucleotide sequence shown in SEQ ID NO: 2 or 13; (D) a nucleotide sequence comprising a deletion, addition or substitution of one or several nucleotides with respect to the nucleotide sequence shown in SEQ ID NO: 2 or 13, and encoding a protein having an ability of synthesizing fatty acid having trans-11-, cis-13-conjugated double bonds from fatty acid having a double bond at position Δ12; and (E) a nucleotide sequence hybridizing with the nucleotide sequence shown in SEQ ID NO: 2 or 13 or a complementary sequence thereof under stringent conditions, and encoding a protein having an ability of synthesizing fatty acid having trans-11-, cis-13-conjugated double bonds from fatty acid having a double bond at position Δ12.

专利号:US-5514569-A
优先权日:1992-12-23
标 题:Method for enzymatic synthesis of oligonucleotides using phosphate precipitation
发明人：HYMAN EDWARD D
摘要:Enzymatic synthesis of a portion of an oligonucleotide is performed by a cycle of synthetic steps: (a) combining an oligonucleotide primer and a blocked nucleotide in a reaction mixture in the presence of a chain extending enzyme, such that a primer-blocked nucleotide product is formed, wherein the blocked nucleotide substrate comprises (i) a nucleotide to be added to form part of the defined sequence and (ii) a blocking group attached to the nucleotide effective to prevent the addition of more than one blocked nucleotide to the primer; and (b) removing the blocking group from the 3' end of the primer-blocked nucleotide product to form a primer-nucleotide product. Phosphate is generated in at least one synthetic step. A precipitate is formed in the cycle comprising phosphate and at least one precipitation cation. The precipitation of phosphate reduces its unfavorable effect on the method. Preferably, cycles of the method are repeated without intermediate purification of primer-nucleotide product or precursor. The precipitation cation may be a polyvalent elemental cation, spermine, or a cation which forms a poorly soluble salt with phosphate. Preferably, the chain extending enzyme is RNA Ligase, the blocked nucleotide is AppNp, and the blocking group is removed using a phosphatase. There is also provided a method for reducing the inhibitory effect of nucleoside 5'-monophosphate and of 3',5'-nucleoside diphosphate on phosphodiesterase I. This is accomplished by enzymatically converting these inhibitors to less inhibitory products.

专利号:WO-2018198543-A1
优先权日:2017-04-28
标题 :Reaction mixture for cell-free protein synthesis, cell-free protein synthesis method in which same is used, and kit for cell-free protein synthesis
发明人：HOMMA Toshimasa
权利人:SPIBER INC
摘要:The present invention pertains to a reaction mixture for cell-free protein synthesis, said reaction mixture including a cell extract and inosinic acid. The present invention also pertains to a cell-free protein synthesis method in which said reaction mixture is used. The present invention furthermore pertains to a kit for cell-free protein synthesis, said kit including a cell extract, inosinic aid, a template nucleic acid, and a substrate and/or energy source for synthesis of the target protein.