CAS: 179101-49-6; 5-Pa-Dutp
该化合物是一种经过修改的脱氧三磷酸类似物,在5个位置上具有3-氨-1-丙基苯替代物,这种修改增强了其在核酸研究中的效用,特别是在需要贴标签或功能化DNA探测器的应用方面的效用.氨基-丙基乙烯组为进一步的融合提供了一个反应式处理器,便利了含氟磷,生物锡或其他报告分子的附加.其三磷酸三酯形式确保通过聚合酶与DNA链中的酶融合相兼容,使其对测序,PCR和基于荧光谱的试验很有价值.该化合物的结构稳定性和选择性再活性使它成为先进的分子生物学和诊断应用的多用途工具.
- 英文名称5-Pa-Dutp
- 中文名称5-(3-氨基-1-丙炔-1-基)-2'-脱氧尿苷5'-(四氢三磷酸酯)
- IUPAC名称((2R,3S,5R)-5-(5-(3-aminoprop-1-yn-1-yl)-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate
- 其它别名5-(3-Amino-1-Propyn-1-Yl)-2'-Deoxyuridine 5'-(Tetrahydrogen Triphosphate); Ap-Dutp; 5-(3-Amino-1-Propyn-1-Yl)-2'-Deoxyuridine 5'-Triphosphate Sodium Salt; Ap-Dutp·3Tea; 5-(3-Amino-1-Propyn-1-Yl)-2'-Deoxyuridine; Uridine 5'-(Tetrahydrogen Triphosphate), 5-(3-Amino-1-Propyn-1-Yl)-2'-Deoxy-; Uridine 5'-(Tetrahydrogen Triphosphate),5-(3-Amino-1-Propynyl)-2'-Deoxy-; 5-Pa-Dutp
- CAS编号179101-49-6
- MFCD编号:MFCD28134652
- 分子式:C12H18N3O14P3分子量:521.21
- 产品CID: 921686
- 产品分类有机原料→生命科学→核苷&核苷酸→修饰核苷酸
- 相似结构搜索
- InChIKey: BGGIMXKBIBMKBL-IVZWLZJFSA-N
- BGGIMXKBIBMKBL-IVZWLZJFSA-N
- 计算化学: 氢键受体数12.0氢键供体数7.0可旋转键数8.0
相似化合物
27821-54-1 365-07-1 365-08-2物理性质
MSDS等安全信息
上下游产品
2'-脱氧-5-[3-[(三氟乙酰基)氨基]-1-丙炔基]尿苷 5-(3''-Trifluoroacetamidopropynyl)-2'-Deoxyuridine 115899-40-6碘苷 5-Iodo-2'-Deoxyuridine 54-42-2
合成工艺路线路线简述
📜碘苷置于磷酸三甲酯,Ammonium Hydroxide,Copper(L) Iodide,四(三苯基膦)钯,1,8-双二甲氨基萘,三乙胺,三氯氧磷体系中,用 N,N-二甲基甲酰胺 用作溶剂,化学反应 11.67H,反应生成5-(3-氨基-1-丙炔-1-基)-2'-脱氧尿苷5'-(四氢三磷酸酯)
参考文献:用于高密度可变 Dna 功能化的多功能工具箱
标题:用于高密度可变 Dna 功能化的多功能工具箱
摘要:为了扩大化学修饰 Dna 在纳米和生物技术,材料科学,传感器开发和分子识别中的适用性,需要将大量不同的修饰同时引入同一核酸序列的策略.在这里,我们使用各种化学修饰的三磷酸脱氧核苷酸 (Dntp),Dna 聚合酶和模板,研究通过引物延伸和 Pcr 获得功能化 Dsdna 的范围和限制.每条链中的所有天然核碱基都被多达四种不同的碱基修饰类似物取代.我们研究了酶促扩增的序列依赖性,以从修饰的 Dntp 和 Fdna 模板中产生高密度功能化 Dna (Fdna),并发现与富含 At 的序列相比,富含 Gc 的序列扩增效率降低.对所使用的聚合酶也有很强的依赖性.虽然 A 族聚合酶通常在包含特定碱基连续片段的"要求高"的模板上表现不佳,但 B 族聚合酶更适合此目的,尤其是 Pwo 和 Vent (Exo-) Dna 聚合酶.通过 Cd 光谱对以增加的密度进行修饰的 Fdna 进行系统分析表明,无论其化学性质如何,单个修饰碱基都不会改变整体
Doi:10.1021/ja051725B
海关参考信息
- 2901292000-乙炔
2905122000-异丙醇
2905310000-1,2-乙二醇
2905320000-1,2-丙二醇 - 💡 提示：海关信息按照顺序优先匹配，如需确认的海关信息，请参考相关资料。
- 详情请参考：📖 海关编码查询和海关进出口税则
供应商参考报价(招募中)
品牌试剂参考报价(招募中)
📌 第三方产品分析报告
主要参考文献
参考标题:Synthesis Of Polyanionic C5-Modified 2′-Deoxyuridine And 2′-Deoxycytidine-5′-Triphosphates And Their Properties As Substrates For Dna Polymerases作者:Claire Dutson,Esther Allen,Mark J. Thompson,Joseph H. Hedley,Heather E. Murton,David M. Williams
摘要:Modified 2′-Deoxyribonucleotide Triphosphates (Dntps) Have Widespread Applications In Both Existing And Emerging Biomolecular Technologies. For Such Applications It Is An Essential Requirement That The Modified Dntps Be Substrates For Dna Polymerases. To Date Very Few Examples Of C5-Modified Dntps Bearing Negatively Charged Functionality Have Been Described, Despite The Fact That Such Nucleotides Might Potentially Be Valuable In Diagnostic Applications Using Si-Nanowire-Based Detection Systems. Herein We Have Synthesised C5-Modified Dutp And Dctp Nucleotides Each Of Which Are Labelled With An Dianionic Reporter Group. The Reporter Group Is Tethered To The Nucleobase Via A Polyethylene Glycol (Peg)-Based Linkers Of Varying Length. The Substrate Properties Of These Modified Dntps With A Variety Of Dna Polymerases Have Been Investigated To Study The Effects Of Varying The Length And Mode Of Attachment Of The Peg Linker To The Nucleobase. In General, Nucleotides Containing The Peg Linker Tethered To The Nucleobase Via An Amide Rather Than An Ether Linkage Proved To Be The Best Substrates, Whilst Nucleotides Containing Peg Linkers From Peg6 To Peg24 Could All Be Incorporated By One Or More Dna Polymerase. The Polymerases Most Able To Incorporate These Modified Nucleotides Included Klentaq, Vent(Exo-) And Therminator, With Incorporation By Klenow(Exo-) Generally Being Very Poor.
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