专利号:US-7344863-B2 优先权日:1998-03-13 标题:Methods for producing polypeptides through enhanced synthesis of encoding nucleic acid molecules 发明人:LI WU-BO; JESSEE JOEL A; SCHUSTER DAVID; XIA JIULIN; GEBEYEHU GULILAT 权利人:INVITROGEN CORP 摘要:The present invention is directed to compositions and methods for enhancing synthesis of nucleic acid molecules, particularly GC-rich nucleic acid molecules. Specifically, the invention provides compositions comprising one or more nitrogen-containing organic compounds having a formula selected from the group consisting of formula I and formula II (or salts or derivatives thereof), preferably 4-methylmorpholine N-oxide or betaine (carboxymethyltrimethylammonium), and further comprising one or more compounds selected from the group consisting of proline and an N-alkylimidazole compound, and more preferably proline, 1-methylimidazole or 4-methylimidazole. The invention further relates to methods for enhanced, high-fidelity synthesis of nucleic acid molecules, including via amplification (particularly PCR), reverse transcription, and sequencing methods. The invention also relates to nucleic acid molecules synthesized by these methods, to fragments or derivatives thereof, and to vectors and host cells comprising such nucleic acid molecules, fragments, or derivatives. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the compositions of the invention.
专利号:US-9788548-B2 优先权日:2013-12-13 标题:Synthesis of pure diallyl morpholinium monomers in high yields and using antibacterial effect of their spiro polymers 发明人:BICAK TUGRUL CEM; INCE AHMET; BICAK NIYAZI 权利人:BICAK TUGRUL CEM; Tugrul Cem BICAK 摘要:Herein, the synthesis of N,Ndiallyl morpholinium monomers and polymerization of the same to form rings at high yield and purity are explained. The process involves the synthesis of N,Ndiallyl morpholinium bromide and chloride and subsequently partially or completely exchanging their anions with borate, p-toluenesulfonate, oleate, and acetate anions. The cyclopolimerization of monomers yields water soluble polymers carrying quaternary ammonium groups in each repeated unit, whose aqueous solutions act as a bactericide solution. These solutions are advantageous in preparation of antibacterial formulations intended for domestic use. The polymer with bromide and borate anions is an efficient antibacterial which is able to kill “ Pseudomonas Aeruginosa â€?, the hardest hospital bacterium to cope with, as well as various common bacteria. These formulations are suitable for producing bactericide wet wipes and forming abacterial surfaces and, when combined with air conditioners, generating bacteria free air.
专利号:US-2025214849-A1 优先权日:2023-12-28 标 题:Synthesis of porous crystalline materials in the presence of water-soluble oxidized disulfide oil and fluoride mineralizer 发明人:HODGKINS ROBERT PETER; KOSEOGLU OMER REFA 权利人:SAUDI ARABIAN OIL CO 摘要:Methods are provided for hydrothermal synthesis of porous crystalline materials. The method generally comprises forming a solution of precursors and reagents in effective ratios for a porous crystalline material including a fluoride-containing mineralizer, and water-soluble oxidized disulfide oil. The solution is hydrothermally treated under effective conditions and for an effective time to synthesize porous crystalline materials.
专利号:US-2025214851-A1 优先权日:2023-12-28 标 题 :SYNTHESIS OF POROUS CRYSTALLINE MATERIALS IN THE PRESENCE OF FLUORIDE MINERALIZER AND pH-MODIFIED WATER-SOLUBLE OXIDIZED DISULFIDE OIL 发明人:HODGKINS ROBERT PETER; KOSEOGLU OMER REFA 权利人:SAUDI ARABIAN OIL CO 摘要:Methods are provided for hydrothermal synthesis of porous crystalline materials. The method generally comprises forming a solution of precursors and reagents in effective ratios for a porous crystalline material including a fluoride-containing mineralizer, and a pH-modified water-soluble oxidized disulfide oil composition. The solution is hydrothermally treated under effective conditions and for an effective time to synthesize porous crystalline materials.
专利号:US-11407993-B2 优先权日:2017-02-14 标 题 :Method for the synthesis of DNA conjugates by micellar catalysis 发明人:BHAT AVINASH SHASHIDHAR; KLIKA SKOPIC MATEJA; BRUNSCHWEIGER ANDREAS; WEBERSKIRCH RALF 权利人:UNIV DORTMUND TECH 摘要:A method for the synthesis of a chimeric conjugate molecule by micellar catalysis that may form part of DNA-encoded compound libraries. A DNA-coupled organic starter molecule may be reacted with another organic compound, using a catalyst located within a micelle, to form a conjugate of an organic candidate compound coupled to a DNA identifier tag.
专利号:US-2025214848-A1 优先权日:2023-12-28 标 题:Synthesis of b-oriented mfi in the presence of water-soluble oxidized disulfide oil and fluoride mineralizer 发明人:HODGKINS ROBERT PETER; KOSEOGLU OMER REFA 权利人:SAUDI ARABIAN OIL CO 摘要:Methods are provided for hydrothermal synthesis of b-oriented MFI zeolite. The method generally comprises forming a solution of precursors and reagents in effective ratios for a b-oriented MFI zeolite including a fluoride-containing mineralizer, and water-soluble oxidized disulfide oil. The solution is hydrothermally treated under effective conditions and for an effective time to synthesize b-oriented MFI zeolite.
参考文献:10.1021/bm200808p 摘要:Ouyang X, Deng Y, Qian Y, Zhang P, Qiu X. Adsorption characteristics of lignosulfonates in salt-free and salt-added aqueous solutions. Biomacromolecules. 2011 Sep 12;12(9):3313–20. doi: 10.1021/bm200808p.