CAS: 1226-39-7; 4-Nitrophenyl β-D-Fucopyranoside
该化合物是一个合成胶合胶层,通常用作生物化学鉴定的基质,特别是用于检测 fucosidase 酶,该化合物具有一种fucapranoside modie, 一种来自fucose的糖,一种与4-硝基苯组有关联的4-硝基苯基-D-氟正拉诺赛面;硝基苯组的存在允许进行色度检测,因为它可能受到烟雾的水解,释放4-硝基酚,这显示出一种截然不同的黄色溶液.这种特性使得其在酶动能研究和评估各种生物样品的酶活性方面很有价值.该化合物通常在水和有机溶剂中溶解,便于在各种实验条件下使用.此外,必须小心处理这种物质,因为如果摄取或吸入,可能会对健康造成危害,在实验室使用时应采取适当的安全措施.
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18918-31-5 35599-02-1 3767-28-0物理性质
MSDS等安全信息
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📜4-Nitrophenol Sodium Salt置于sodium Methylate体系中,用 甲醇,N,N-二甲基甲酰胺 用作溶剂,化学反应生成4-硝基苯-Beta-D-吡喃半乳糖苷
参考文献:Synthesis Of P-Nitrophenyl 2-O-α-And-β-L-Fucopyranosyl-β-D-Fucopyranoside
标题:Synthesis Of P-Nitrophenyl 2-O-α-And-β-L-Fucopyranosyl-β-D-Fucopyranoside
摘要:
Doi:10.1016/0008-6215(83)88286-X
海关参考信息
- 2905122000-异丙醇
2905310000-1,2-乙二醇
2905320000-1,2-丙二醇
2905399001-1,3-丙二醇 - 💡 提示：海关信息按照顺序优先匹配，如需确认的海关信息，请参考相关资料。
- 详情请参考：📖 海关编码查询和海关进出口税则
专利信息
专利号:EP-0725144-A1
优先权日:1995-02-06
标题 :Process for the preparation of glycosides using glycosidases from ciliates
发明人：KIY THOMAS DR; HOERSCH BRIGITTE DR; MARQUARDT RUEDIGER DR
权利人:HOECHST AG
摘要:The invention relates to a process for the production of glycosides by enzymatic glycosylation by means of α- and β-glycosidases from holotrich ciliates and in particular from Hymenostomatida.n n n Glycosidases from holotrich ciliates are very well suited for the enzymatic synthesis of alkyl glycosides, glycopeptides, di- or oligosaccharides. Preferred glycosyldoren are nitrophenylglycosides, glycosyl fluorides or disaccharides, preferred acceptors are short-chain alcohols, polyhydroxy compounds, in particular monosaccharides, or protected hydroxyamino acids or corresponding peptides.
专利号:US-11371032-B2
优先权日:2018-06-05
标题 :Beta glucosidase with high glucose tolerance, high thermal stability and broad PH activity spectrum
发明人：MARJAMAA KAISA; KOIVULA ANU; ARO NINA; PAKULA TIINA
权利人:TEKNOLOGIAN TUTKIMUSKESKUS VTT OY
摘要:The present invention discloses a polypeptide having beta-glucosidase activity. The activity is retained also in high glucose concentration. The invention also discloses an isolated polynucleotide, a nucleic acid construct and a recombinant host usable in production of said polypeptide, and a method for producing the polypeptide. Further the invention discloses compositions including the polypeptide and method of using the polypeptide in hydrolysis or synthesis.
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主要参考文献
[参考文献]: Beatrice Cobucci-Ponzano, Et Al. Identification Of The Catalytic Nucleophile Of The Family 29 Alpha-L-Fucosidase From Sulfolobus Solfataricus Via Chemical Rescue Of An Inactive Mutant. Biochemistry. 2003 Aug 19;42(32):9525-31.[参考文献]: Francisco De La Torre, Et Al. Atfxg1, An Arabidopsis Gene Encoding Alpha-L-Fucosidase Active Against Fucosylated Xyloglucan Oligosaccharides. Plant Physiol. 2002 Jan;128(1):247-55.
[参考文献]: K Ajisaka, Et Al. Regioselective Synthesis Of Alpha-L-Fucosyl-Containing Disaccharides By Use Of Alpha-L-Fucosidases Of Various Origins. Carbohydr Res. 1992 Feb 7:224:291-9.
[参考文献]: Karen M Polizzi, Et Al. Pooling For Improved Screening Of Combinatorial Libraries For Directed Evolution. Biotechnol Prog. 2006 Jul-Aug;22(4):961-7.
[参考文献]: M A Cohenford, Et Al. Colorimetric Assay For Free And Bound L-Fucose. Anal Biochem. 1989 Feb 15;177(1):172-7.
合成参考文献
参考文献:10.1093/glycob/cwp196
摘要:Benesová E, Lipovová P, Dvoráková H, Králová B. beta-D-Galactosidase from Paenibacillus thiaminolyticus catalyzing transfucosylation reactions. Glycobiology. 2010 Jan;20(4):442–51. doi: 10.1093/glycob/cwp196.
参考文献:10.1590/s0100-879x2009007500033|10.1590/s0100-879x2010000100002
摘要:Tomassi MH, Rozenfeld JH, Gonçalves LM, Marana SR. Characterization of the interdependency between residues that bind the substrate in a beta-glycosidase. Braz J Med Biol Res. 2010 Jan;43(1):8–12. doi: 10.1590/s0100-879x2009007500033.
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