专利号:US-2001049117-A1 优先权日:1998-08-20 标 题 :Analogs of udp-murnac peptides, assays, kits and related methods of their use 发明人:AXELROD HELENA R; BRANSTROM ARTHUR A 摘要:General compositions and methods for detecting Lipid I, Lipid II and peptidoglycan synthesis is disclosed. A method of screening for potential antibacterial agents is provided which requires bacterial membrane preparations or enriched enzyme preparations including at least one bacterial enzyme involved in the synthesis of Lipid I from UDP-MurNAc pentapeptide and undecaprenyl phosphate, at least one bacterial enzyme involved in the synthesis of Lipid II from Lipid I and UDP-GlcNAc and one or more bacterial enzymes involved in the further processing of Lipid II toward the downstream synthesis of peptidoglycan. The methods disclosed herein further provides a labeled UDP-MurNAc-peptide capable of serving as a substrate for a bacterial enzyme involved in the synthesis of Lipid I and a labeled UDP-GlcNAc capable of serving as a substrate for a bacterial enzyme involved in the synthesis of Lipid II. Conditions for further processing of Lipid II toward the downstream synthesis of peptidoglycan are also discribed.
专利号:US-8067204-B2 优先权日:2005-12-15 标 题 :Long-chain chondroitin sugar chain and method for producing the same and method for promoting synthesis of chondroitin 发明人:SUGIURA NOBUO; SHIMOKATA SATOSHI; KIMATA KOJI 权利人:SUGIURA NOBUO; SHIMOKATA SATOSHI; KIMATA KOJI; SEIKAGAKU KOGYO CO LTD 摘要:A method for producing a chondroitin sugar chain comprises reacting a glucuronic acid donor, an N-acetyl galactosamine donor, a sugar receptor and a bacterial cell enzyme which synthesizes chondroitin in the presence of a surfactant. The surfactant is selected from polyoxyethylene octadecyl amine, n-decanoyl-N-methylglucamide, sodium cholate, n-octyl-β-D-thioglucopyranoside, n-nonyl-β-D-thiomaltopyranoside, sucrose monocholate, sucrose monocaprate, and sucrose monolaurate. The chondroitin sugar chain has all the following properties: a weight average molecular weight: 50,000 or more when measured by gel filtration chromatography; it is completely degraded to disaccharides with chondroitinase ABC; and when the sugar chain is decomposed with chondroitinase ABC and the decomposed products are subjected to a disaccharide analysis, substantially all of them correspond to an unsaturated disaccharide unit of chondroitin.
专利号:US-2009263867-A1 优先权日:2005-12-15 标 题 :Long-chain chondroitin sugar chain and method for producing the same and method for promoting synthesis of chondroitin
专利号:EP-1964924-B1 优先权日:2005-12-15 标 题 :Long-chain chondroitin sugar chain and method for producing the same and method for promoting synthesis of chondroitin
专利号:US-9617533-B2 优先权日:2011-03-10 标题:Composition for synthesizing protein with reduced lipopolysaccharide contamination, method for producing protein using said composition 发明人:KANAMORI TAKASHI; HAYAMI YUKI; KOJOH KANEHISA; UEDA TAKUYA; TSUIHIJI KUMIKO; FUSE TOMOE; NAKAMURA MIKIKO; KATO SHIZUE 权利人:GENEFRONTIER CORP; UNIV TOKYO; THE UNIVERISTY OF TOKYO 摘要:According to the present invention, a composition possessing cell-free protein synthesis activity with reduced contaminating lipopolysaccharide, and a method for producing a protein using the same are provided. When ribosome display is performed using the composition and method for protein production of the present invention, the background that is caused by non-specific binding is reduced, so that a nucleic acid that encodes the desired polypeptide can be selected with high accuracy and high efficiency.
专利号:EP-1964924-A1 优先权日:2005-12-15 标 题:Long-chain chondroitin sugar chain and method for producing the same and method for promoting synthesis of chondroitin
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合成参考文献
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