专利号:US-2023313255-A1 优先权日:2020-07-15 标题:Massively Parallel Enzymatic Synthesis of Polynucleotides 发明人:HORGAN ADRIAN; LACHAIZE HENRI; VERARDO DOMIANO; GODRON XAVIER 权利人:DNA SCRIPT 摘要:The invention is directed to methods and compositions for inkjet assisted synthesis of a plurality of polynucleotides at reaction sites on a substrate using template-free polymerases, such as, terminal deoxynucleotidyl transferases (TdTs). Compositions of the invention include formulations of synthesis reagents for inkjet delivery including, but not limited to, TdT coupling reaction buffers and 3′-O-protected dNTP monomers.
专利号:WO-2024153643-A1 优先权日:2023-01-16 标 题 :Inkjet-assisted enzymatic nucleic acid synthesis 发明人:MOGHADDAM NICOLAS; ADELIZZI BEATRICE; LOMAN TESSA; VERARDO DAMIANO; RODRIGUEZ-PINZON DANIEL; HORGAN ADRIAN 权利人:DNA SCRIPT 摘要:Methods, devices, and compositions are provided for inkjet-assisted synthesis of a plurality of polynucleotides at reaction sites on a substrate using template-free polymerases, such as, terminal deoxynucleotidyl transferases (TdTs). Compositions include printable stabile formulations of synthesis reagents for inkjet delivery including, but not limited to, template-free polymerases, divalent cations, and nucleotides.
专利号:US-8877171-B2 优先权日:2010-02-03 标 题 :Polyanionic multivalent macromolecules for intracellular targeting of proliferation and protein synthesis 发明人:LICHA KAI; SCHIRNER MICHAEL; WELKER PIA; HAAG RAINER; WEINHART MARIE; PAULUS FLORIAN 权利人:LICHA KAI; SCHIRNER MICHAEL; WELKER PIA; HAAG RAINER; WEINHART MARIE; PAULUS FLORIAN; MIVENION GMBH 摘要:The present invention relates generally to methods and compositions for targeting of intracellular molecules involved in proliferation and protein synthesis of activated cells using polyanionic multivalent macromolecules. In particular aspect, multiple sulfate groups linked to polyol are specifically targeted to the cytoplasm and nucleus of proliferating and activated cells. The invention further comprises novel polyanionic macromolecular compounds and formulations.
专利号:US-7417142-B2 优先权日:2002-03-28 标 题:Chiral porphyrins, chiral metalloporphyrins, and methods for synthesis of the same 发明人:ZHANG X PETER; CHEN YING; GAO GUANG-YAO 权利人:UNIV TENNESSEE RES FOUNDATION 摘要:Novel methods of synthesizing heteroatom-containing chiral porphyrins and chiral metalloporphyrins and the novel chiral porphyrins and chiral metalloporphyrins themselves are disclosed. Metal complexes of the chiral porphyrins are prepared in high yields and shown to be active catalysts for highly enantioselective and diastereoselective cyclopropanation, aziridination, and epoxidation of alkenes under a practical one-pot protocol.
专利号:US-5187103-A 优先权日:1991-01-31 标题:Colorimetric method and reagent for the assay of lithium in a test sample 发明人:CZECH BRONISLAW P; CHAPOTEAU EDDY; KUMAR ANAND 权利人:MILES INC 摘要:A chromogenic cryptand found to be especially selective in the determination of lithium in blood of the general formula (I): ##STR1## where: n=1 or 2, n R 1 and R 2 , same or different, are hydrogen, lower alkyl, lower alkenyl, or lower alkylidene; and n Q is a chromogenic moiety capable of providing a detectable response upon complexation of said compound with lithium ion and has the structure: ##STR2## wherein: X is CH, C--OH or N; and Z is p-nitrophenylazo, 2,4-dinitrophenylazo, 2,4,6-trinitrophenylazo, p-nitrostyryl, p-benzoquinonemonoimino, bis-(4-dimethylaminophenyl) hydroxymethyl, 3-phenylisothiazolyl-5-azo, thiazolyl-5-azo; or isothiazolyl-5-azo. n A reagent composition and synthesis of a preferred chromogenic cryptand are also disclosed.
专利号:WO-2024141628-A1 优先权日:2022-12-31 标 题:Variable viscosity inks for inkjet delivery of enzyme reagents 发明人:HORGAN ADRIAN; VERARDO DAMIANO; ADELIZZI BEATRICE 权利人:DNA SCRIPT 摘要:The invention is directed to formulations of terminal deoxynucleotidyl transferases (TdTs) inks employing variable viscosity modifiers and their use in inkjet assisted synthesis of a plurality of polynucleotides at reaction sites on a substrate.
1: Bassis CM, Visick KL. The cyclic-di-GMP phosphodiesterase BinA negatively regulates cellulose-containing biofilms in Vibrio fischeri. J Bacteriol. 2010 Mar;192(5):1269-78. doi: 10.1128/JB.01048-09. Epub 2010 Jan 8. 2: Behnajady MA, Modirshahla N, Shokri M, Zeininezhad A, Zamani HA. Enhancement photocatalytic activity of ZnO nanoparticles by silver doping with optimization of photodeposition method parameters. J Environ Sci Health A Tox Hazard Subst Environ Eng. 2009 Jun;44(7):666-72. doi: 10.1080/10934520902847752. doi: 10.1080/10934520701796267.
合成参考文献
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