CAS: 13187-90-1; (S)-2-((S)-2-Aminopropanamido)Pentanedioic Acid

上下游产品

N-(N-benzyloxycarbonyl-L-alanyl)-L-glutamic acid dibenzyl esterN-(N-benzyloxycarbonyl-L-alanyl)-L-glutamic acid dibenzyl ester dibenzyl L-glutamate B°C-Ala-Glu(OMe)-OMe B°C-Ala-Glu-OHL-alanin L-glutamic acid

专利信息

专利号:US-6994986-B2
优先权日:1999-03-17
标 题:In vitro synthesis of polypeptides by optimizing amino acid metabolism
发明人：SWARTZ JAMES ROBERT; KIM DONG-MYUNG
权利人:UNIV LELAND STANFORD JUNIOR
摘要:Compositions and methods are provided for the enhanced in vitro synthesis of polypeptides. In order to improve the performance of in vitro protein synthesis reactions, metabolic inhibitors, or manipulation of a source organism, is used to diminish or avoid the action of enzymes responsible for undesirable amino acids production or depletion. A homeostatic system may be used for production of ATP, where the required high energy phosphate bonds are generated in situ, e.g. through coupling with an oxidation reaction. The homeostatic energy source will typically lack high energy phosphate bonds itself, and will therefore utilize free phosphate in the reaction mix during generation of ATP. The homeostatic energy source is provided in combination with an enzyme that catalyzes the creation of high energy phosphate bonds and with an enzyme that can use that high energy phosphate bond to regenerate ATP.

专利号:US-11518794-B2
优先权日:2016-08-19
标 题:Synthesis method for liraglutide with low racemate impurity
发明人：FU YUQING; MA HONGJI; LI XINYU; ZHANG LIXIANG; ZHI QIN; WU LIFEN; LIU ZICHENG
权利人:SHENZHEN JYMED TECH CO LTD
摘要:A synthesis method for low-racemization impurity liraglutide comprises the following steps: performing synthesis to obtain a propeptide, coupling 2 to 5 peptides comprising Thr-Phe on the propeptide by using a solid-phase synthesis method; further, performing solid-phase synthesis to obtain a liraglutide resin; the liraglutide resin is cracked after modification, or the liraglutide resin is directly cracked, purified and frozen dry, so as to obtain the liraglutide. The provided liraglutide synthesis method effectively restrains or reduces the generation of racemization impurity D-Thr 5 highly similar to a product property, which facilitates the purification of the coarse liraglutide, and the high yield of the liraglutide is ensured, thereby greatly reducing production costs; during the synthesis of the liraglutide, the syntheses between dipeptide fragments, tripeptide fragments, the tetrapeptide fragments and pentapeptide fragments and the Gly-resin or the syntheses between the combination of the dipeptide fragments, the tripeptide fragments, the tetrapeptide fragments and pentapeptide fragments and the Gly resin can be carried out at the same time, and accordingly the synthesis time is shortened to some extent.

专利号:US-2024279705-A1
优先权日:2021-08-03
标题 :Eukaryotic cell lysates comprising exogenous enzymes and methods for preparing the same
发明人：SCHLOSSHAUER JEFFREY; ZEMELLA ANNE; KUBICK STEFAN; CAVAK NINO; THORING LENA
权利人:FRAUNHOFER GES FORSCHUNG
摘要:The present invention relates to a method for producing eukaryotic cell lysates for cell-free protein synthesis comprising at least one exogenous enzyme, wherein the method comprises at least the following steps: a) providing eukaryotic cells transfected with at least one donor template coding for at least one exogenous enzyme which is selected from the group comprising orthogonal aminoacyl-tRNA synthetases and viral RNA polymerases: b) cultivating the transfected cells of step a) for a predetermined period of time and subsequently harvesting the cells; and c) disrupting the harvested cells and preparing a cell lysate for cell-free protein synthesis therefrom. More specifically, the invention relates to a method for producing eukaryotic cell lysates which are capable of cell-free synthesis of a target protein comprising a non-canonical amino acid, wherein the method comprises at least the following steps: a) providing eukaryotic cells transfected with at least one donor template coding for an orthogonal aminoacyl-tRNA synthetase. i.e. an aminoacyl-tRNA synthetase which is specific for a tRNA which is not recognized by endogenous aminoacyl-tRNA synthetase in said eukaryotic cells and is specific for a corresponding non-canonical amino acid: b) cultivating the transfected cells of step a) for a predetermined period of time and subsequently harvesting the cells; and c) disrupting the harvested cells and preparing a cell lysate therefrom. Further aspects of the present invention relate in particular to eukaryotic cell lysates obtainable by the above methods as well as to a method for performing cell-free synthesis of a target protein comprising a non-canonical amino acid.

专利号:CA-2394414-A1
优先权日:1999-12-16
标 题 :Moss genes from physcomitrella patens encoding proteins involved in the synthesis of tocopherols carotenoids

专利号:CA-2355402-A1
优先权日:1998-12-21
标题:Recombinant synthesis of beta-lipotropin and other peptides

专利号:CA-2513289-A1
优先权日:2003-01-20
标 题:Expression cassette with promotors of starch synthesis 3 for the expression of nucleic acids in plant tissue containing starch