CAS: 6815-91-4; Gdp-L-Galactose

上下游产品

Guanosine 5'-Diphospho-D-Mannose
Guanosine 5'-Triphosphate 86-01-1
Gmp-Morpholidate 7390-53-6

📜Guanosine 5'-Diphospho-D-Mannose置于tris-Hcl Buffer,Nicotinamide Adenine Dinucleotide体系中,化学反应生成 Gdp-L-Gulose,[5-(2-氨基-6-氧代-3,6-二氢-9H-嘌呤-9-基)-3,4-二羟基四氢-2-呋喃基]甲基 3,4,5-三羟基-6-(羟基甲基)四氢-2H-吡喃-2-基二氢二磷酸酯
参考文献:Characterization Of A Gdp-D-Mannose 3'',5''-Epimerase From Rice
标题:Characterization Of A Gdp-D-Mannose 3'',5''-Epimerase From Rice
摘要:The Enzymatic Characterization Of Gdp-D-Mannose 3",5"-Epimerase (Gme),A Key Enzyme In The Biosynthesis Of Vitamin C In Plants Is Described. The Gme Gene (Genbank Accession No. Ab 193582) In Rice Was Cloned,And Expressed As A Fusion Protein In Escherichia Coli. Reaction Products From Gdp-D-Mannose,As Produced By Gme Catalysis,Were Separated By Recycling HPLC On An Ods Column,And Were Determined To Be Gdp-L-Galactose And Gdp-L-Gulose,Based On Their NMR Spectra And Sugar Analysis. The Reaction Catalyzed By Gme Was Inhibited By Gdp,And Was Strongly Accelerated By Nad(+) In Contrast To The Case Of Gme From Arabidopsis Thaliana. This Difference In The Effect Of Nad(+) On Gme Activity Can Be Attributed To The Nad Binding Domain Which Is Conserved In The Rice Gene,But Not In The Arabidopsis Thaliana Gene. The Apparent K-M And K(Cat) Were Determined To Be 1.20 X 10(-5) M And 0.127 S(-1),Respectively,In The Presence Of 20 Mu M Nad(+). The Fractions Of Gdp-D-Mannose,Gdp-L-Galactose And Gdp-L-Gulose,At Equilibrium,Were Approximately 0.75,0.20 And 0.05,Respectively. (C) 2005 Elsevier Ltd. All Rights Reserved.
DOI:10.1016/j.Phytochem.2005.12.003

专利信息

专利号:US-2002142422-A1
优先权日:1999-12-16
标 题 :Moss genes from physcomitrella patens encoding proteins involved in the synthesis of amino acids, vitamins, cofactors, nucleotides and nucleosides
发明人：LERCHL JENS; RENZ ANDREAS; EHRHARDT THOMAS; REINDL ANDREAS; CIRPUS PETRA; BISCHOFF FRIEDRICH; FRANK MARKUS; FREUND ANNETTE; DUWENIG ELKE; SCHMIDT RALF-MICHAEL; RESKI RALF
摘要:Isolated nucleic acid molecules, designated MP protein nucleic acid molecules, which encode novel MP proteins from e.g. Phycomitrella patens are described. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing MP protein nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further provides isolated MP proteins, mutated MP proteins, fusion proteins, antigenic peptides and methods for the improvement of production of a desired compound from transformed cells, organisms or plants based on genetic engineering of MP protein genes in these organisms.

专利号:US-2006156431-A1
优先权日:2003-07-18
标题 :L-gulose dependent vitamin C synthesis
发明人：WOLUCKA BEATA
权利人:UNIV GENT
摘要:The present invention relates to L-gulose dependent vitamin C synthesis. More specifically, the present invention relates to a synthesis pathway of vitamin C, comprising the formation of GDP-L-gulose by GDP-mannose 3″,5″-epimerase, and subsequent transformation into L-gulose and L-ascorbic acid.

专利号:WO-02103001-A1
优先权日:2001-06-15
标 题 :Gdp-mannose-3',5'-epimerase and methods of use thereof
发明人：WOLUCKA BEATA
权利人:VLAAMS INTERUNIV INST BIOTECH; WOLUCKA BEATA
摘要:The identification of a new key enzyme in vitamin C synthesis, and its use to modulate vitamin C synthesis in eukaryotic cells are disclosed. Specifically, the isolation of a multimeric GDP-mannose-3',5'-epimerase, the identification, cloning and expression of a nucleic acid sequence encoding GDP-mannose-3',5'-epimerase, methods of producing GDP-mannose-3',5'-epimerase, transgenic plants and microorganisms that express the GDP-mannose-3',5'-epimerase, and methods of production of ascorbic acid using the GDP-mannose-3',5'-epimerase are disclosed.

专利号:US-2015225758-A1
优先权日:2011-11-08
标 题 :Genetically modified cells and methods for making activated sugar-nucleotides
发明人：BAR-PELED MAOR; YANG TING
权利人:UNIV GEORGIA
摘要:This disclosure generally relates to genetically engineered cells and methods of making and using such genetically engineered cells. Generally, the genetically engineered cells exhibit an increase in synthesis of an activated sugar-nucleotide compared to a wild type control. In some embodiments, the activated sugar-nucleotide produced by the genetically engineered cell is an activated sugar-nucleotide that is not natively synthesized by wild type, un-engineered cell. In some embodiments, the activated sugar-nucleotide is an activated uridine diphosphate sugar nucleotide. In other embodiments, the activated sugar-nucleotide is an activated cysteine monophosphate sugar nucleotide. In still other embodiments, the activated sugar-nucleotide is an activated guanosine diphosphate sugar nucleotide. In some embodiments, the activated sugar-nucleotide includes an isotopic label.