CAS: 5959-95-5; (R)-2,5-Diamino-5-Oxopentanoic Acid

相似化合物

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上下游产品

CAS号38062-69-0 h-leu-gln-oh | CAS号2650-69-3 H-Gly-Gln-Gly-OH

Dl-谷氨酰胺置于fatty Acid Photodecarboxylase From Chlorella Variabilis Nc64A/g462Y Mutant体系中,用 Aq. Phosphate Buffer,二甲基亚砜 作为反应溶剂,化学反应 12.0H,以99%的收率获得产物
参考文献:工程脂肪酸光脱羧酶可实现α-官能化羧酸的光驱动动力学拆分.
标题:工程脂肪酸光脱羧酶可实现α-官能化羧酸的光驱动动力学拆分.
摘要:手性α-官能化羧酸是多种药物和天然产物的宝贵前体.本文中,我们描述了一种工程化的脂肪酸光脱羧酶(cv Fap)催化的α-氨基酸和α-羟基酸的动力学拆分,可为未反应的r构型底物提供高收率和出色的立体选择性(ee高达99%).这种高效的光驱动过程既不需要nadph回收,也不需要事先进行酯的制备,而这是以前的生物催化方法所必需的.结构指导的工程策略基于在热点处扫描大氨基酸以缩小底物结合通道.据我们所知,这是一个由工程人员设计的不对称催化的第一个例子.简历fap.
DOI:10.1002/anie.201903165

专利信息

专利号:US-12421534-B2
优先权日:2021-11-10
标 题 :Engineered enzymes and method for the synthesis of diverse tyrosine analogs
发明人：ALMHJELL PATRICK J; ARNOLD FRANCES H
权利人:CALIFORNIA INST OF TECHN
摘要:Provided herein is an engineered tryptophan synthase β-subunit (TrpB) that catalyzes the synthesis of tyrosine, tyrosine analogs, or salts thereof. Also provided herein are methods for preparing tyrosine, tyrosine analogs, or a salt thereof using the engineered TrpB described herein.

专利号:US-12024731-B2
优先权日:2017-05-03
标 题 :Methods and enzyme catalysts for the synthesis of non-canonical amino acids
发明人：BOVILLE CHRISTINA E; BRINKMANN-CHEN SABINE; BULLER ANDREW R; ROMNEY DAVID K; PRIER CHRISTOPHER K; KOCH PHILIPP; SCHEELE REMKES A
权利人:CALIFORNIA INST OF TECHN
摘要:The present disclosure provides methods for preparing β-substituted tryptophan compounds. The methods include: combining i) an unsubstituted indole or a substituted indole, ii) a β-substituted serine, and iii) a tryptophan synthase β-subunit (i.e., a TrpB); and maintaining the resulting mixture under conditions sufficient to form the β-substituted tryptophan. The TrpB contains at least one amino acid mutation which promotes formation of an amino-acrylate intermediate. New TrpB variants and new β-substituted tryptophan analogs are also described.

专利号:US-10829792-B2
优先权日:2017-03-21
标 题 :Biocatalytic synthesis of strained carbocycles
发明人：CHEN KAI; HUANG XIONGYI; KAN S B JENNIFER
权利人:CALIFORNIA INST OF TECHN
摘要:Provided herein are methods for producing products containing strained carbocycles, such as cyclopropene moieties and/or bicyclobutane moieties. The methods include combining an alkyne and a carbene precursor in the presence of a heme protein, e.g., a cytochrome P450, under conditions sufficient to form the strained carbocycle. Reaction mixtures for producing strained carbocycles are also described, as well as whole-cell catalysts comprising heme proteins and variants thereof for forming cyclopropenes, bicyclobutanes, and related products.

专利号:US-10526379-B2
优先权日:2015-06-05
标题:Methods and products for fusion protein synthesis
发明人：HOWARTH MARK
权利人:UNIV OXFORD INNOVATION LTD
摘要:A method of producing a fusion protein is provided. The method includes: a) contacting a first protein with a second protein under conditions that enable the formation of an isopeptide bond between said proteins to form a linked protein; and b) contacting the linked protein from (a) with a third protein under conditions that enable the formation of an isopeptide bond between said third protein and said linked protein to form a fusion protein. Peptide linkers and the use of orthogonal pairs of said linkers in the synthesis of fusion proteins are also provided. Recombinant proteins comprising said linkers, nucleic acid molecules encoding said proteins and linkers, vectors comprising said nucleic acid molecules and host cells comprising said vectors and nucleic acid molecules are also contemplated.

专利号:US-2022233673-A1
优先权日:2019-06-04
标 题:METHODS OF PRODUCING SHIGA TOXIN B-SUBUNIT (STxB) MONOMERS AND OLIGOMERS, AND USES THEREOF
发明人：BILLET ANNE; SCHMIDT FRÉDÉRIC; JOHANNES LUDGER; SERVENT DENIS; MOURIER GILLES; TARTOUR ÉRIC; KAY MICHAEL; FULCHER JAMES M
权利人:INST CURIE; CENTRE NAT RECH SCIENT; INST NAT SANTE RECH MED; COMMISSARIAT A LENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES CEA; APHP ASSIST PUBLIQUE HOPITAUX DE PARIS; UNIV PARIS; UNIV OF UTAH RESEARCH FOUDATION; UNIV UTAH RES FOUND
摘要:A method of producing a monomer of a Shiga toxin B-subunit (STxB) protein or of a variant thereof by peptide chemical synthesis, as well as to a method of producing a pentamer of the STxB protein or of the variant thereof. The methods are particularly advantageous as they overcome major issues typically observed in peptide chemical synthesis, including solubility and purity issues.

专利号:US-2024409571-A1
优先权日:2023-06-08
标 题:Chemoenzymatic synthesis of n-acetylated gangliosides and glycosphingosines
发明人：CHEN XI; YU HAI; MISHRA BIJOYANANDA; ZHENG ZIMIN; AGRAHARI ANAND KUMAR; GUCCHAIT ARIN
权利人:UNIV CALIFORNIA
摘要:Described herein are novel stable 9-N-, 8-N-, and 7-N-acetyl analogues of instable 9-O-acetyl, 8-O-acetyl, and 7-O-acetyl b-series gangliosides and glycosphingosines, including GD3, GD2, GD1b, GT1b, GQ1b, and their glycosphingosines. Chemoenzymatic methods for the production of the stable 9-N-, 8-N-, and 7-N-acetyl analogues are also described herein.