专利号:US-2002192767-A1 优先权日:1999-10-13 标题 :Biosynthesis of polyketide synthase substrates 发明人:KHOSLA CHAITAN; PFEIFER BLAINE 摘要:The use of enzymes which catalyze the production of starter and extender units for polyketides is described. In addition, modified loading modules are described, which can accept a variety of starting units such as substituted benzoates, and which can be used to generate substituted derivatives of natural products. These enzymes may be used to enhance the yield of polyketides that are natively produced or polyketides that are rationally designed. By using these techniques, the synthesis of a complete polyketide has been achieved in E. coli . Production can be enhanced in microbial organisms of polyketides and other secondary metabolites by delaying production until after exponential phase and by maintaining relatively constant nutrient levels in the medium. In addition, polyketide production can be enhanced by providing an expression system for a thioesterase II. Thus, by modifying the host organism and changing the culture conditions, synthesis of a secondary metabolite can be enhanced. The present invention also results in a host organism with desirable characteristics to be used in the production of such polyketides and to assess the results of gene shuffling.
专利号:WO-2017105352-A1 优先权日:2015-12-18 标 题 :Synthesis of inorganic sio2 microcapsules containing phase change materials and applications therein 发明人:YANG JINGLEI; AN JINLIANG; YANG EN-HUA 权利人:UNIV NANYANG TECH 摘要:Disclosed herein are microcapsules that encapsulate a phase change material, where the microcapsules have a dual-core structure with a first shell being made of an organic polymeric material, in particular polyurea surrounded by a second inorganic material shell, in particular silica. Also disclosed herein is a process to manufacture said microcapsules and applications thereof.
专利号:US-10711022-B2 优先权日:2017-12-26 标 题 :Methods of producing oligosaccharides for use as prebiotics 发明人:PAN XUEJUN; LI NING 权利人:WISCONSIN ALUMNI RES FOUND 摘要:Provided herein are methods for preparing prebiotic oligosaccharides by non-enzymatic methods of glycosylation of monosaccharides and/or disaccharides or by hydrolysis of polymeric sugars to monosaccharides and simultaneous glycosylation of hydrolytic products. The methods may include mixing one or more types of monosaccharides and/or disaccharides with a water-deficient system at a temperature sufficient to form one or more types of prebiotic oligosaccharides. Also provided are methods of preparing prebiotic oligosaccharides comprising mixing starch, cellulose and/or lignocellulosic biomass with a water-deficient system at a temperature sufficient to form one or more types of prebiotic oligosaccharides that collectively make up at least 25% of the products of the synthesis.
专利号:CN-1321734-C 优先权日:2005-03-29 标题:Synthesis of Inorganic Phase Change Energy Storage Microcapsules by Solution Precipitation Polymerization
专利号:US-11739357-B2 优先权日:2017-07-07 标 题 :Recombinant microorganism for producing citicoline and method for producing citicoline 发明人:JIANG JUNJUN; FAN JUNYING; TIAN FENG; WANG XINTONG; ZHANG KAILIN; HU ZHIHAO 权利人:SUZHOU BIOSYNTHETICA CO LTD 摘要:The present invention provides a recombinant microorganism for producing citicoline and a method for producing citicoline by using the recombinant microorganism, wherein genes for degradation and utilization of citicoline, choline, and phosphocholine are knocked out, In addition, a pyrimidine nucleoside synthesis pathway is genetically engineered to remove feedback inhibition to the synthesis pathway. A yield of more than 20 g/L of citicoline can be obtained with recombinant strains in a 5-liter fermenter by means of a biological fermentation method, achieving industrial mass production with low citicoline production costs and less pollution; therefore, the method is a simple, environmentally friendly and has a relatively high promotion and application value.
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合成参考文献
摘要:Naunyn-Schmiedeberg's Archiv fuer Experimentelle Pathologie und Pharmakologie., 131(171), 1928