CAS: 3650-93-9; 4-Amino-2-Hydroxy-5-Pyrimidinecarboxylic Acid

相似化合物

20737-41-1 769-52-8 1517617-07-0

上下游产品

CAS号774-07-2 6-氨基-2-硫代-1,2-二... | CAS号778-97-2 2-乙硫基-4-氨基嘧啶-5-羧酸乙酯 | CAS号23945-44-0 脲嘧啶-5-羧酸 | CAS号71-30-7 胞嘧啶 | CAS号66-22-8 尿嘧啶

📜5-甲基-2'-脱氧胞苷置于盐酸,水,Ce(4+)*6No3(1-)*c30H27Fen5O(2+)体系中,化学反应 63.0H,反应生成2-羟基-4-氨基嘧啶-5-羧酸
参考文献:仿生铁复合物实现 Tet 酶反应性**
标题:仿生铁复合物实现 Tet 酶反应性**
摘要:表观遗传标记 5-甲基-2'-脱氧胞苷 (5Mdc) 是最常见的 Dna 修饰.它尤其通过主动去甲基化途径被去除:通过10-11易位5-甲基胞嘧啶双加氧酶(Tet)氧化,随后通过碱基切除修复或直接解修饰去除.最近,我们发现合成的铁(Iv)-氧配合物[fe Iv (O)(Py 5 Me 2 H)] 2+ ( 1 )可以通过氧化核碱基5-甲基胞嘧啶作为tet的仿生模型(5Mc) 与其天然代谢物.在这项工作中,我们使用一系列 HPLC 和 Ms 技术证明核苷甚至短寡核苷酸链也可以作为底物.我们发现5Mc的5位优选被1氧化,副反应仅发生在所用寡核苷酸的链末端.对1对核苷反应性的详细研究证实了我们的结果;异头中心 (1') 的氧化是最常见的副反应.
Doi:10.1002/anie.202107277

专利信息

专利号:US-12421628-B2
优先权日:2018-07-23
标题:Massively parallel enzymatic synthesis of nucleic acid strands
发明人：HORGAN ADRIAN; GODRON XAVIER; YBERT THOMAS; NICOL ROBERT
权利人:DNA SCRIPT
摘要:The invention is directed to methods for massively parallel template-free enzymatic synthesis of a plurality of different polynucleotides of predetermined sequences. In one aspect, methods of the invention employ large scale arrays of reaction sites each associated with at least one working electrode for controlling deprotection and deblocking steps at predetermined user selected sites. In another aspect, the invention provides template-free enzymatic synthesis with proofreading, wherein completed polynucleotides at predetermined reaction sites are sequenced using a sequencing by synthesis technique, particularly employing electrochemically labile blocking groups.

专利号:US-2024052391-A1
优先权日:2020-10-29
标 题 :Enzymatic Synthesis of Polynucleotide Probes
发明人：GODRON XAVIER; NIYOMCHON SUPAPORN; CHAMPION ELISE
权利人:DNA SCRIPT
摘要:The present invention is directed to methods and kits for enzymatic synthesis of labeled polynucleotide probes using template-free DNA polymerases.

专利号:US-2024384319-A1
优先权日:2021-09-17
标题 :Synthesis of Oligonucleotides
发明人：LOVELOCK SARAH
权利人:UNIV MANCHESTER
摘要:The present invention relates to a novel method for oligonucleotide synthesis, using a catalytic primer-template, e.g. a hairpin primer, to achieve multiple rounds of oligonucleotide synthesis and dissociation from the template in the same reaction, such that at the end of the reaction the amount of oligonucleotide generated is greater than the amount of template provided in the reaction. The primer contains a cleavable site and the reaction is carried out in a single mixture with a cleavage agent. The cleavable site may be an inosine and the agent an endonuclease V.

专利号:US-2019078126-A1
优先权日:2017-09-08
标 题 :Polymerase-mediated, template-independent polynucleotide synthesis
发明人：BAIGA THOMAS; BURLEY MICHAEL ANDERSON; SMITH ALEXANDER
权利人:SIGMA ALDRICH CO LLC; MERCK PATENT GMBH
摘要:Methods for de novo synthesis of polynucleotides in which 3′-O-reversibly blocked nucleotides are attached to a solid support in the presence of an X family DNA polymerase and in the absence of a nucleic acid template.

专利号:WO-2023196499-A1
优先权日:2022-04-07
标 题:Substrate cleavage for nucleic acid synthesis
发明人：LACKEY JEREMY; DODD DAVID; LANGNER HEERA
权利人:TWIST BIOSCIENCE CORP
摘要:Disclosed herein are methods and compositions for cleavage of nucleic acids from a surface of a solid support. Further described herein are cleavage methods compatible with enzymatic and chemical nucleic acid synthesis methods.

专利号:WO-2025003669-A1
优先权日:2023-06-27
标题 :Polynucleotide synthesis method, kit and system
发明人：CHAPMAN TARUN STEPHEN; CLARKE DAVID EDWARD; CLARKE JAMES ANTHONY; COOK IMOGEN HELEN; MÃœLLER CAROLIN ANNE; OGAKI RYOSUKE; WHITE JAMES PHILIP
权利人:OXFORD NANOPORE TECH PLC
摘要:The invention relates to new methods for synthesising polynucleotide molecules according to a predefined nucleotide sequence. The invention also relates to methods for the assembly of synthetic polynucleotides following synthesis.