Sodium Succinate置于ferrous(II) Sulfate Heptahydrate,硫酸氢铵,Dipotassium Hydrogenphosphate,Potassium Dihydrogenphosphate,Sodium Molybdate Dihydrate,Calcium(II) Chloride Dihydrate,Manganese(II) Chloride Tetrahydrate,Magnesium(II) Chloride Hexahydrate体系中,用 水 用作溶剂,化学反应 97.0H,反应生成甘油三油酸酯 参考文献:通过前体定向生物合成制备的支链 Tag 的 Rp-HPLC/ms-Apci 分析. 标题:通过前体定向生物合成制备的支链 Tag 的 Rp-HPLC/ms-Apci 分析. 摘要:采用反相液相色谱-大气压化学电离质谱(rp-HPLC/ms-Apci)分析分子中含有1-3个支链脂肪酸(fa)的合成三酰甘油(tag)和前体制备的天然tag在红球红球菌的培养中从缬氨酸,亮氨酸和异亮氨酸以及相应的支链短链酸进行定向生物合成.该技术使得识别和量化单个支链 Fa 中不同的 Tag 成为可能.总共合成了 11 个 Tag,其中 8 个是立体定向合成的.支链和直链 Tag 被分离和鉴定,而仅异或反异 Fa 不同的 Tag 无法分离.异链,反异链和直链 Tag 的 Apci 质谱完全相同.发现天然材料含有 19 个具有至少一种支链 Fa 的 Tag.在六种不同底物上的培养表明,除了前体与等偶,异奇和反异 Fa 的假定和常见结合外,还有一些不寻常的特征,例如添加 Val 后奇 Fa 的含量增加(归因于val 分解代谢为丙酸)或支链单不饱和 Fa 的出现.两样本配对 T 检验,当应用于 Tag Doi:10.1007/s11745-010-3447-7
专利号:US-2023174477-A1 优先权日:2021-12-03 标题:Methods for synthesis of the tricyclic prostaglandin d2 metabolite methyl ester 发明人:DAI MINGJI; SIMS HUNTER 权利人:PURDUE RESEARCH FOUNDATION 摘要:Methods for the synthesis of a tricyclic-prostaglandin D 2 metabolite methyl ester or a pharmaceutically acceptable salt thereof.
专利号:US-10604479-B2 优先权日:2013-11-18 标 题:Continuous process for the one-pot synthesis of metal salts and metal complexes 发明人:LEONARDI GIULIANO; GASPERONI GIOVANNI 权利人:VOLARE & CONNECTING LLC; LEONARDI GIULIANO; NOVUS INT INC 摘要:A continuous process is described for the one-pot synthesis of compounds, such as metal salts and metal complexes, as well as optionally for the protection of the compounds thus obtained by coating and/or impregnation with suitable compounds.
专利号:US-9597327-B2 优先权日:2005-05-25 标 题:Synthesis of (R)-N-methylnaltrexone 发明人:DOSHAN HAROLD D; PEREZ JULIO 权利人:PROGENICS PHARM INC 摘要:This invention relates to stereoselective synthesis of R-MNTX and intermediates thereof, pharmaceutical preparations comprising R-MNTX or intermediates thereof and methods for their use.
专利号:US-7473541-B2 优先权日:2004-07-09 标 题:Identification, cloning, expression, and purification of three novel human calcium-independent phospholipase A2 family members possessing triacylglycerol lipase and acylglycerol transacylase activities 发明人:GROSS RICHARD W; JENKINS CHRISTOPHER 权利人:GROSS RICHARD W; JENKINS CHRISTOPHER 摘要:Isolated novel and purified and characterized phospholipase A 2 , referred to herein as calcium-independent phospholipase A 2 zeta (iPLA 2 zeta) having SEQ. ID. NO: 2 (See FIG. 1 ), and nucleic acid sequence (SEQ. ID. NO: 4), and calcium-independent phospholipase A 2 eta (iPLA 2 eta) having SEQ. ID. NO: 3 (See FIG. 1 ), and nucleic acid sequences (SEQ. ID. NO: 5). For the first time herein, these novel enzymes have been isolated and characterized and are involved in the catalysis, synthesis and hydrolysis of lipids in a living mammalian cell. Moreover, these enzymes iPLA 2 zeta and iPLA 2 eta through the process of transesterification can catalyze the net anabolic synthesis of triglycerides through a variety of metabolic precursors (e.g. monoacylglycerol, diacylglycerol and acyl CoA).
专利号:US-2025305010-A1 优先权日:2022-05-08 标题:Lipid compositions and methods of producing same 发明人:KEREN TOMER; ELLA EZRA; ARGOV-ARGAMAN NURIT 权利人:WILK TECH INTERNATIONAL LTD; YISSUM RES DEV CO OF HEBREW UNIV JERUSALEM LTD 摘要:Provided is a method of increasing lipid synthesis or accumulation in mammary epithelial cells (MECs) culture and optionally secretion therefrom. The method comprising contacting the MECs with an LXR agonist in the presence of a fatty acid to thereby increase lipid synthesis or accumulation and optionally secretion of lipids. Also provided is a method of producing synthetic fat globules, the method comprising: (a) isolating lipid droplets from cells in culture; (b) encapsulating the droplets with a lipid bilayer, thereby producing synthetic fat globules. Also provided are compositions produced thereby.
专利号:US-2023065361-A1 优先权日:2021-08-18 标 题:Method for increasing use efficiency of lipase during enzymatic reaction 发明人:WANG XIAOSAN; HUANG YE; Huang Zhuoneng; CHEN YE; LIU KEYING; HUANG YAQI; WANG XIAOHAN; CHANG MING 权利人:UNIV JIANGNAN 摘要:The present disclosure discloses a method for increasing the usage efficiency of a lipase in the enzymatic reaction, belonging to the technical field of food processing. In the present disclosure, the peroxide value of raw materials required for enzymatic synthesis of a structured lipid is reduced by a distillation method or an adsorption method, and then the raw material and a lipase undergo catalytic reaction to synthesize the structured lipid, so as to maintain the activity of the lipase and increase the reuse rate of the enzyme. The distillation method includes deodorization and molecular distillation. The present disclosure provides a method for manufacturing a structured lipid by a high efficiency and low-cost enzymatic synthesis, addressing to the issues such as large enzyme consumption, expansive cost, low enzyme utilization rate in the traditional industrial production. Therefore, the method provided by the present disclosure has certain development potential and practical significance.
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合成参考文献
参考文献:10.1371/journal.pone.0021209 摘要:Brown JD, Oligino E, Rader DJ, Saghatelian A, Plutzky J. VLDL Hydrolysis by Hepatic Lipase Regulates PPARδ Transcriptional Responses. PLoS ONE. 2011 Jul 05;6(7):e21209. doi: 10.1371/journal.pone.0021209. 参考文献:10.2147/ceg.s17634 摘要:Fieker A, Philpott J, Armand M. Enzyme replacement therapy for pancreatic insufficiency: present and future. Clin Exp Gastroenterol. 2011;4():55–73. 参考文献:10.1186/1475-2859-10-54 摘要:Glogauer A, Martini VP, Faoro H, Couto GH, Müller-Santos M, Monteiro RA, Mitchell DA, de Souza EM, Pedrosa FO, Krieger N. Identification and characterization of a new true lipase isolated through metagenomic approach. Microbial Cell Factories. 2011 Jul 15;10(1):54. doi: 10.1186/1475-2859-10-54. 参考文献:10.1016/j.ijbiomac.2011.07.001 摘要:Narang J, Pundir CS. Construction of a triglyceride amperometric biosensor based on chitosan–ZnO nanocomposite film. International Journal of Biological Macromolecules. 2011 Nov;49(4):707–15. doi: 10.1016/j.ijbiomac.2011.07.001. 参考文献:10.1016/j.bpj.2011.03.055 摘要:Mitsche M, Small D. C-Terminus of Apolipoprotein A-I Removes Phospholipids from a Triolein/Phospholipids/Water Interface, but the N-Terminus Does Not: A Possible Mechanism for Nascent HDL Assembly. Biophysical Journal. 2011 Jul;101(2):353–61. doi: 10.1016/j.bpj.2011.03.055.