专利号:US-5419966-A 优先权日:1991-06-10 标 题 :Solid support for synthesis of 3'-tailed oligonucleotides 发明人:REED MICHAEL W; MEYER JR RICH B; PETRIE CHARLES R; TABONE JOHN C 权利人:MICROPROBE CORP 摘要:A solid support for oligonucleotide synthesis has the structure where CPG represents a controlled pore glass matrix, the wavy line represents a carbon chain covalently linking the NH group with the controlled pore glass matrix, X is 2,2'-dimethoxytrityl or H, and R is alkyl, aryl, arylalkyl, heteroalkyl, or heteroaryl. The dimethoxytrityl group is removed from the solid support by treatment with acid, and the oligonucleotide is built, step-by-step in a conventional synthesizer after attachment of the 3' end of the first oligonucleotide unit to the hydroxyl function connected to the R group.
专利号:WO-9203464-A1 优先权日:1990-08-28 标题 :Solid support synthesis of 3'-tailed oligonucleotides via a linking molecule
专利号:EP-0547142-A1 优先权日:1990-08-28 标题 :Solid support synthesis of 3'-tailed oligonucleotides via a linking molecule