📜1,1,3,3-Tetramethylbutylphenolate Sodium置于lithium Aluminium Tetrahydride体系中,用 四氢呋喃,二甲基亚砜 用作溶剂,化学反应生成辛基酚聚醚-3 参考文献:Isolation Of Bacterial Strains That Produce The Endocrine Disruptor,octylphenol Diethoxylates,In Paddy Fields 标题:Isolation Of Bacterial Strains That Produce The Endocrine Disruptor,octylphenol Diethoxylates,In Paddy Fields 摘要:从日本西部的36个不同水稻田收集了表层土壤样本.每个土壤样本都跟含有0.2% Oppeo的基础盐培养基一起孵育.十二个样本表现出oppeo降解活性,分别从中分离出十二种oppeo降解细菌.所分离的细菌在含有0.2% Oppeo作为唯一碳源的培养基上生长,在好氧条件下积累了op2Eo和op3Eo.在本实验中,通常与op2Eo一起在地表水中被识别的op1Eo和辛基酚并未被观测到.通过biolog和16S Rdna Rflp分析,所分离的细菌为革兰氏阴性,初步鉴定为假单胞菌(pseudomonas Putida,10个分离株)和伯克霍尔德菌(burkholderia Cepacia,一个分离株). Doi:10.1271/bbb.66.1792
专利号:US-2012190064-A1 优先权日:2004-10-01 标题 :Feeding buffers, systems, and methods for in vitro synthesis of biomolecules 发明人:KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA 权利人:KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA; LIFE TECHNOLOGIES CORP 摘要:Compositions, methods and kits for in vitro systems for synthesis of biomolecules such as polypeptides, are provided herein. Cell extracts that provide enhanced yields of soluble proteins using in vitro protein synthesis methods are provided. The invention also includes methods for producing high yields of proteins by the addition of a feeding solution that includes amino acids and an energy source to an ongoing in vitro synthesis system. The invention also includes methods of using a high-yield in vitro synthesis system to produce large quantities of proteins with incorporated labeled amino acids for analysis by methods such as by NMR. The invention further includes vectors for enhanced production of proteins from nucleic acid templates using in vitro synthesis systems.
专利号:US-2025009786-A1 优先权日:2021-09-30 标 题 :Automated synthesis of polymeric dual drugs 发明人:MATRAY TRACY; VANBRUNT MICHAEL; MCCUTCHEON JOHN MICHAEL 权利人:SONY GROUP CORP 摘要:Compounds useful as biologically active compounds with or without fluorescent or colored dyes are disclosed. In some embodiments, the compounds have the following structure (I): (I) or a stereoisomer, tautomer or salt thereof, wherein R1, R2, R3, R4, R5, R6, R7, L1, L2, L3, L4, L5, L6, L7, L8, L9, L10, L11, M1, M2, M3, l, m, n, p, and q are as defined herein. Additional compound, methods of preparation, pharmaceutical compositions, and methods of treatment related to compounds of Structure (I) are also provided.
专利号:US-9068192-B2 优先权日:2009-01-28 标 题:Compositions and methods for conversion of aldehydes to alkanes 发明人:ZENG WEIQING; HE SHENG YANG 权利人:ZENG WEIQING; HE SHENG YANG; UNIV MICHIGAN STATE 摘要:The invention provides the discovery that SCD2 (Susceptible to Coronatine-Deficient Pst DC3118-2) protein converts aldehydes to alkanes, thereby changing the composition and/or amount of synthesized waxes. The invention additionally provides homologs, orthologs and paralogs of SCD2 protein, and nucleotide sequences encoding these proteins. Also provided are expression vectors, transgenic cells, transgenic plants, transgenic seeds, methods for altering alkane production in cells, methods for identifying the function of a nucleotide sequence in the synthesis of plant surface wax, and methods for identifying plant tissue that has an altered surface wax composition. The invention's compositions and methods are useful for altering the amount and/or composition of wax produced by cells, including plants and seeds, that have been transformed with the invention's sequences.
专利号:WO-2004083431-A1 优先权日:2003-03-19 标题:Method of screening protein capable of controlling transcription reaction and method of assaying activity thereof 发明人:KAWAI KUNJI; NAKA DAIJI; SUZUKI KATSUHIKO; NAKANO HIROFUMI; KONDO JUN 权利人:ZOEGENE CORP; KAWAI KUNJI; NAKA DAIJI; SUZUKI KATSUHIKO; NAKANO HIROFUMI; KONDO JUN 摘要:A method of screening a transcription associated factor characterized in that the synthesis of test protein is carried out in a cell-free protein synthetic system and the function of transcription associated factor is analyzed on the basis of, as an indicator, an interaction or change of interaction between the transcription associated factor and a DNA having a base sequence for transcription control region.
专利号:US-11033507-B2 优先权日:2013-12-19 标 题 :Method of synthesis of silica vesicles and use thereof 发明人:YU CHENGZHONG; MITTER NEENA; ZHANG JUN 权利人:UNIV QUEENSLAND 摘要:The invention relates, in part, to a method of producing silica vesicles including under controlled conditions to thereby heavily influence the morphology and characteristics of the vesicles. The vesicles are shown to be effective as delivery agents for chemical and biological agents. They are also shown to be useful in methods of treatment and as components of an immunogenic composition.
专利号:US-7816081-B2 优先权日:2005-04-28 标 题 :Method of minimizing reagent consumption in microplate-based reactions 发明人:MITSUHASHI MASATO; OGURA MIEKO 权利人:HITACHI CHEMICAL CO LTD; HITACHI CHEMICAL RES CT INC 摘要:A method is provided for performing a reaction, such as the synthesis of concentrated cDNA, in the wells of a microplate while minimizing the volume of the solution of reagents required to perform the reaction. In the method, a pestle is inserted into the well of a microplate to which a substance has been immobilized. A volume of reagent solution is introduced into the well that is insufficient to cover the portion of the well onto which the substance is immobilized. The insertion of the pestle displaces reagent solution and increases the surface area of the solution in contact with the portion of the well to which the substance has been immobilized when the pestle is inserted.