该化合物是一种天然的甘蓝,主要来自Siraitia grosvenorii的水果,通常称为和尚水果,以其强烈的甜度闻名,比苏格罗塞的甜度大得多,使它在食品和饮料应用中成为受欢迎的天然甜度. Mogroside II E 的特点是其低热量含量,因为它不像传统糖那样被身体代谢,从而帮助寻求更健康替代品的人.复合氧化剂的特性,可能带来额外的健康福利.化学上,它属于一种被称为mogrosides的化合物,即三叶素沙邦,其结构包括附在三叶脊骨上的多种糖浆,有助于其甜度和功能特性. Mogroside II E 被普遍承认为安全的消费用途,并且正在继续研究其潜在的健康利益和各种饮食产品的应用.
📜Mogroside V置于pectinase From Aspergillus Aculeatus,水体系中,用 Aq. Acetate Buffer 作为反应溶剂,化学反应 24.0H,以18.8 Mg的收率获得产物罗汉果苷iie 参考文献: High Intensity Sweeteners[fr] édulcorants à Intensité élevée 标题: High Intensity Sweeteners[fr] édulcorants à Intensité élevée
专利信息
专利号:US-12071646-B2 优先权日:2014-09-11 标 题 :Cells comprising mogroside pathway enzymes and uses thereof 发明人:ITKIN MAXIM; DAVIDOVICH-RIKANATI RACHEL; COHEN SHAHAR; PORTNOY VITALY; DORON-FAIGENBOIM ADI; PETREIKOV MARINA; SHEN SHMUEL; TADMOR YAAKOV; BURGER YOSEF; LEWINSOHN EFRAIM; KATZIR NURIT; SCHAFFER ARTHUR A; OREN ELAD 权利人:THE STATE OF ISRAEL MINISTRY OF AGRICULTURE & RURAL DEVELOPMENT AGRICULTURAL RES ORGANIZATION ARO VO 摘要:Isolated mogroside and mogrol biosynthetic pathway enzyme polypeptides useful in mogroside biosynthesis are provided. Mogroside biosynthetic pathway enzymes of the invention include squalene epoxidase (SE), epoxy hydratase (EH), cytochrome p450 (Cyp), cucurbitadienol synthase (CDS) and udp-glucosyl-transferase (UGT), Also provided are methods of producing a mogroside using the isolated mogroside and mogrol biosynthetic enzyme polypeptides, the methods comprising contacting a mogrol and/or a glycosylated mogrol (mogroside) with at least one UDP glucose glucosyl transferase (UGT) enzyme polypeptide of the invention catalyzing glucosylation of the mogrol and/or the glucosylated mogrol to produce a mogroside with an additional glucosyl moietie(s), thereby producing the mogroside. Alternatively or additionally provided is a method of synthesizing a mogrol, the method comprising contacting a mogrol precursor substrate with one or more mogrol biosynthetic pathway enzyme polypeptides as described herein catalyzing mogrol synthesis from the mogrol precursor substrate, thereby synthesizing the mogrol.
参考文献:10.1248/cpb.38.2030 摘要:MATSUMOTO K, KASAI R, OHTANI K, TANAKA O. Minor cucurbitane-glycosides from fruits of Siraitia grosvenori (Cucurbitaceae). Chem. Pharm. Bull. 1990;38(7):2030–2. doi: 10.1248/cpb.38.2030. 参考文献:10.1021/np068074x 摘要:Akihisa T, Hayakawa Y, Tokuda H, Banno N, Shimizu N, Suzuki T, Kimura Y. Cucurbitane Glycosides from the Fruits of Siraitia grosvenorii and Their Inhibitory Effects on Epstein−Barr Virus Activation. J. Nat. Prod. 2007 May 04;70(5):783–8. doi: 10.1021/np068074x.