专利号:US-6133018-A 优先权日:1997-06-02 标 题 :Enzymatic synthesis of chiral amines using -2-amino propane as amine donor 发明人:WU WEI; BHATIA MOHIT B; LEWIS CRAIG M; LANG WEI; WANG ALICE L; MATCHAM GEORGE W 权利人:CELGRO 摘要:2-Aminopropane is used as the amine donor in the stereoselective synthesis of a chiral amine from a ketone with a transaminase. In a typical embodiment, (S)-1-methoxy-2-aminopropane is prepared by bringing methoxyacetone into contact with a transaminase in the presence of 2-aminopropane as an amine donor until a substantial amount of methoxyacetone is converted to (S)-1-methoxy-2-aminopropane and 2-aminopropane is converted to acetone. In a second embodiment, L-alanine is prepared by bringing pyruvic acid into contact with a transaminase in the presence of 2-aminopropane as an amine donor.
专利号:EP-1075534-B8 优先权日:1998-03-11 标 题 :Improvements in the enzymatic synthesis of chiral amines 发明人:WU WEI; BHATIA MOHIT B; LEWIS CRAIG M; LANG WEI; WANG ALICE; MATCHAM GEORGE W 权利人:CELGRO 摘要:2-Aminopropane is used as the amine donor in the stereoselective synthesis of a chiral amine from a ketone with a transaminase. In a typical embodiment, (S)-1-methoxy-2-aminopropane is prepared by bringing methoxyacetone into contact with a transaminase in the presence of 2-aminopropane as an amine donor until a substantial amount of methoxyacetone is converted to (S)-1-methoxy-2-aminopropane and 2-aminopropane is converted to acetone. In a second embodiment, L-alanine is prepared by bringing pyruvic acid into contact with a transaminase in the presence of 2-aminopropane as an amine donor.
专利号:WO-2018157942-A1 优先权日:2017-03-03 标题:Cell-free synthesis of isotopic labelled proteins from amino-acids precursors 发明人:CRUBLET ELODIE; IMBERT LIONEL; GANS PIERRE; BOISBOUVIER JÉRÔME; KERFAH RIME 权利人:NMR BIO; COMMISSARIAT ENERGIE ATOMIQUE; CENTRE NAT RECH SCIENT; UNIV GRENOBLE ALPES 摘要:Process of cell-free synthesis of a target protein, comprising at least the following steps: a. providing at least one precursor of an amino-acid, a cell-free extract comprising means for transforming said precursor of an amino-acid into the corresponding amino-acid, at least one tRNA for integrating said amino-acid into the corresponding target protein, in which the at least one precursor of amino-acid is an alpha-keto acid, with the proviso that 2-ketobutyric acid is excluded, b. mixing the compounds provided in step a) to obtain a mixture, c. adding to said mixture obtained in step b) a DNA or a mRNA coding to said target protein in order to produce said target protein.
专利号:WO-2023198025-A1 优先权日:2022-04-11 标题:Synthesis method and synthesis device for organic nitrogen-containing compound 发明人:LI GUANGQIN; Liao Peisen; XIAN JIAHUI; LI SUISHENG; ZHANG YAWEI 权利人:UNIV SUN YAT SEN 摘要:The present invention relates to the field of organic synthesis, specifically to a synthesis method and synthesis device for an organic nitrogen-containing compound. Provided in the present invention is a synthesis method for an organic nitrogen-containing compound. In the method provided in the present invention, a porous carbon material is used as a catalyst, organic nitrogen-containing compounds such as an oxime, an amine, a nitrile and an amino acid are synthesized by means of an electro-catalysis method, byproducts are unlikely to generate, and the method is safe and environmentally friendly. Experiments show that organic nitrogen-containing compounds such as an amino acid, an oxime, an amine and a nitrile are successfully synthesized by using the method of the present invention, and the method has good Faraday efficiency and yield. In the present application, the synthesis of the above organic nitrogen-containing compounds can be realized by using nitrogen oxide waste gas or waste water as a raw material; and by converting the nitrogen oxide waste gas or waste water, the utilization of waste is realized, and the benefits are maximized. Further provided in the present invention is a synthesis device for an organic nitrogen-containing compound, which device is used for solving the defects of high energy consumption, long consumption time, and complex product separation and purification of existing synthesis methods.
专利号:US-6066780-A 优先权日:1991-04-26 标题 :Modification of lignin synthesis in plants 发明人:BOUDET ALAIN MICHEL; INZE DIRK GUSTAAF; SCHUCH WOLFGANG WALTER 权利人:ZENECA LTD 摘要:The synthesis of lignin by plants is controlled by transformation of the plant genome with a recombinant gene construct which contains the gene specifying an enzyme critical to the synthesis of a lignin precursor, which gene may be in antisense orientation so that it is transcribed to mRNA having a sequence complementary to the equivalent mRNA transcribed from the endogenous gene thus leading to suppression of lignin synthesis. If the recombinant gene has the lignin enzyme gene in normal, or 'sense' orientation, increased production of the enzyme may occur when the insert is the full length DNA but suppression may occur it only a partial sequence is employed.
专利号:US-5202235-A 优先权日:1986-08-18 标题 :Enzymatic method for the synthesis and separation of peptides 发明人:IACOBUCCI GUILLERMO A 权利人:COCA COLA CO 摘要:The present invention provides a method for the enzymatic synthesis of peptides accomplished by shifting the chemical equilibrium that exists in a reaction mixture between charged or ionized reacting amino acids and uncharged or non-ionized peptide product in the presence of a proteolytic enzyme such as thermolysin. The equilibrium is shifted by diffusion of the uncharged peptide product across an ion-rejection membrane which removes the uncharged peptide from the reaction mixture and preferably the diffused uncharged peptide is quickly converted to a charged species that cannot back-diffuse into the reaction mixture so that the uncharged peptide is effectively 'pulled' across the membrane.
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合成参考文献
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