CAS: 2365-40-4; N6-Dimethylallyladenine

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利波腺苷置于recombinant Solanum Tuberosum Cytokinin Riboside Phosphorylase体系中,用 Aq. Buffer 用作溶剂,化学反应 0.33H,反应生成N6-异戊烯基腺嘌呤
参考文献:A Purine Nucleoside Phosphorylase In Solanum Tuberosum L. (Potato) With Specificity For Cytokinins Contributes To The Duration Of Tuber Endodormancy
标题:A Purine Nucleoside Phosphorylase In Solanum Tuberosum L. (Potato) With Specificity For Cytokinins Contributes To The Duration Of Tuber Endodormancy
摘要:Stckp1 (solanum Tuberosum 细胞分裂素核糖苷磷酸化酶)催化植物激素 Ck(细胞分裂素)的 N9-核糖苷形式(嘌呤的一个子集)与其最具活性的游离碱基形式之间的相互转化.Stckp1 更喜欢 Ck 而不是未取代的氨基嘌呤.该蛋白是在马铃薯块茎化匍匐茎尖的提取物中发现的,并通过亲和层析法从中分离出具有 Ck 结合活性的蛋白.其 N 端氨基酸序列与一组 Ests 的翻译产物相匹配,从而能够通过 Race-Pcr 获得完整的 Mrna 序列.预测的多肽包括一个可裂解的信号肽和嘌呤核苷磷酸化酶活性基团.对表达的蛋白质进行了嘌呤核苷磷酸化酶活性检测,检测对象为 Cks 和腺嘌呤/腺苷.在 1-磷酸核糖存在的情况下,异戊烯基腺嘌呤,反式玉米素,双氢玉米素和腺嘌呤被转化为核苷.与此相反,异戊烯基腺苷,反式玉米素核苷,二氢玉米素核苷和腺苷在 Pi 的存在下转化为其游离碱基.Stckp1 没有检测到核糖水解酶活性.有证据表明,Stckp1 在块茎中作为 Cks 的负调控因子具有活性,可通过冷可逆机制延长内眠期.
Doi:10.1042/bj20130792

专利信息

专利号:US-11858953-B2
优先权日:2018-04-04
标 题:Compositions and methods for synthesis of phosphorylated molecules
发明人：CHAPUT JOHN; LIAO JEN-YU; BALA SAIKAT
权利人:UNIV CALIFORNIA
摘要:The invention provides compositions and methods for synthesis of phosphorylated organic compounds, including nucleoside triphosphates.

专利号:US-10973847-B2
优先权日:2017-06-30
标题 :Core-to-surface polymerization for the synthesis of star polymers and uses thereof
发明人：JOHNSON JEREMIAH A; GOLDER MATTHEW R
权利人:MASSACHUSETTS INST TECHNOLOGY
摘要:Disclosed are methods, compositions, reagents, systems, and kits to prepare star polymers, as well as compositions and uses thereof. Various embodiments show that synthesis of these polymers contain low metal concentration to provide polymers for diverse biomedical applications including in vivo applications.

专利号:US-2019323050-A1
优先权日:2016-12-21
标题 :Modulation of Enzymatic Polynucleotide Synthesis Using Chelated Divalent Cations
发明人：GRISWOLD JR KETTNER JOHN FREDERICK; LEE HOWON; CHURCH GEORGE M
权利人:HARVARD COLLEGE
摘要:Methods and apparatus of modulating polynucleotide synthesis are provided. The methods include delivering reagents comprising enzymes, nucleotides and ions to oligonucleotide primers wherein the reagents catalyze incorporation of the nucleotides to 3′ ends of the oligonucleotide primers, and modulating incorporation of the nucleotides to the 3′ ends of the oligonucleotide primers. Polynucleotide synthesis is modulated by modulating presence or absence of catalytic cation cofactors to provide sequence defined synthesis of polynucleotides. In certain embodiments, the polynucleotides encode information.

专利号:US-2002161219-A1
优先权日:2001-02-21
标 题:Non-enzymatic large scale synthesis of RNA
发明人：KANAVARIOTI ANASTASSIA; DEAMER DAVID W; MONNARD PIERRE-ALAIN M; BERNASCONI CLAUDE F
摘要:This invention pertains to the development of methods that accomplish efficient, non-enzymatic, nucleic acid-directed (e.g. RNA-directed) nucleic acid (e.g. RNA) synthesis. In certain embodiments the methods provide conditions that favor oligouridylate synthesis with excellent yield and, at least, up to 30% regioselectivity favoring the RNA linkage. The methods preferably involve contacting, in the presence of lead ions (Pb 2+ ) and/or tin ions (Sn 2+ ) and, optionally, magnesium ions (Mg 2+ ), a template nucleic acid with a nucleotide derivatized with an imidazolide.

专利号:US-11851651-B2
优先权日:2017-06-19
标 题 :Automated methods for scalable, parallelized enzymatic biopolymer synthesis and modification using microfluidic devices
发明人：BANAL JAMES; BERLEANT JOSEPH DON; SHEPHERD TYSON; BATHE MARK
权利人:MASSACHUSETTS INST TECHNOLOGY
摘要:Methods for the automated template-free synthesis of user-defined sequence controlled biopolymers using microfluidic devices are described. The methods facilitate simultaneous synthesis of up to thousands of uniquely addressed biopolymers from the controlled movement and combination of regents as fluid droplets using microfluidic and EWOD-based systems. In some forms, biopolymers including nucleic acids, peptides, carbohydrates, and lipids are synthesized from step-wise assembly of building blocks based on a user-defined sequence of droplet movements. In some forms, the methods synthesize uniquely addressed nucleic acids of up to 1,000 nucleotides in length. Methods for adding, removing and changing barcodes on biopolymers are also provided. Biopolymers synthesized according to the methods, and libraries and databases thereof are also described. Modified biopolymers, including chemically modified nucleotides and biopolymers conjugated to other molecules are described.

专利号:US-5414077-A
优先权日:1990-02-20
标题 :Non-nucleoside linkers for convenient attachment of labels to oligonucleotides using standard synthetic methods
发明人：LIN KUEI-YING; MATTEUCCI MARK
权利人:GILEAD SCIENCES
摘要:Pseudonucleosides and pseudonucleotides are useful in the synthesis of oligomers which contain these components as a means to derivatize the resulting oligonucleotide to useful substituents such as chelators, intercalators, or lipophilic compounds. In general, these pseudonucleotide components are of the formula: (1) wherein each Y is independently O or S; each X is independently H, PO3-2, an activated nucleotide synthesis coupling moiety, a protecting group, a nucleoside, a nucleotide or a nucleotide sequence, or comprises a solid support; F is a functional group capable of linking an additional moiety or said group already reacted to effect the binding of said additional moiety; &squ& is an organic backbone which does not contain additional F or Y-X substituents and which is either achiral even when the Y-X substituents are different, or is a single enantiomer of a chiral compound; with the proviso that at least one X is a nucleoside, nucleotide, nucleotide sequence, an activated nucleotide synthesis coupling moiety, or comprises a solid support, or F represents said functional group already reacted with an additional group. Oligonucleotides having the pseudonucleoside at the 3' terminus are particularly stable in vivo.