CAS: 192441-08-0; 6-((4-Bromothiophen-2-yl)Methoxy)-9H-Purin-2-Amine

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CAS号79757-77-0 (4-溴-2-噻吩基)甲醇 | CAS号34798-95-3 2-amino-N,N,N-t... | CAS号18791-75-8 4-溴-2-噻吩甲醛

📜4-溴-2-噻吩甲醛置于sodium Tetrahydroborate,Sodium Hydride,二甲基亚砜体系中,用 异丙醇 用作溶剂,化学反应 3.0H,反应生成罗米鲁曲
参考文献:Monosaccharide-Linked Inhibitors Of O6-Methylguanine-Dna Methyltransferase (Mgmt): Synthesis,Molecular Modeling,And Structure−activity Relationships
标题:Monosaccharide-Linked Inhibitors Of O6-Methylguanine-Dna Methyltransferase (Mgmt): Synthesis,Molecular Modeling,And Structure−activity Relationships
摘要:A Series Of Potential Inhibitors Of The Human Dna Repair Protein O-6-Methylguanine-Dna Methyltransferase (Mgmt) Were Synthesized,Characterized In Detail By NMR,And Tested For Their Ability To Deplete Mgmt Activity In Vitro. The New Compounds,Omega-[o-6-R-Guan-9-Yl]-(Ch2)(N)-Beta-D-Glucosides With R = Benzyl Or 4-Bromothenyl And Omega = N = 2,4,... 12,Were Compared With The Established Inhibitors O-6-Benzylguanine (O-6-Bg),8-Aza-O-6-Benzylguanine (8-Aza-Bg),And O-6-(4-Bromothenyl)Guanine (4-Btg),Which Exhibit In An In Vitro Assay Ic50 Values Of 0.62,0.038,And 0.009 Mum,Respectively. Potential Advantages Of The Glucosides Are Improved Water Solubility And Selective Uptake In Tumor Cells. The 4-Btg Glucosides With N = 2,4,6 Show Moderate Inhibition With An Ic50 Of Ca. 0.5 Mum,While Glucosides Derived From Bg And 8-Aza-Bg Showed Significantly Poorer Inhibition Compared To The Parent Compounds. The 4-Btg Glucosides With N = 8,10,12 Were Effective Inhibitors With Ic50 Values Of Ca. 0.03 Mum. To Understand This Behavior,Extensive Molecular Modeling Studies Were Performed Using The Published Crystal Structure Of Mgmt (Pdb Entry: 1Qnt). The Inhibitor Molecules Were Docked Into The Bg Binding Pocket,And Molecular Dynamics Simulations With Explicit Water Molecules Were Carried Out. Stabilization Energies For The Interactions Of Specific Regions Of The Inhibitor And Individual Amino Acid Residues Were Calculated. The Alkyl Spacer Is Located In A Cleft Along Helix 6 Of Mgmt. With Increasing Spacer Length There Is Increasing Interaction With Several Amino Acid Residues Which Play An Important Role In The Proposed Nucleotide Flipping Mechanism Required For Dna Repair.
Doi:10.1021/jm010006E