CAS: 69075-47-4; 4-Amino-5-Ethynyl-1-((2R,4S,5R)-4-Hydroxy-5-(Hydroxymethyl)Tetrahydrofuran-2-yl)Pyrimidin-2(1H)-One

上下游产品

2'-脱氧胞嘧啶核苷 2'-Deoxycytidine 951-77-9
5-碘-2'-脱氧胞苷 5-Iodo-2'-Deoxycytidine 611-53-0

📜5-碘-2'-脱氧胞苷置于甲醇,Bis-Triphenylphosphine-Palladium(II) Chloride,Copper(L) Iodide,Potassium Carbonate,三乙胺体系中,用 N,N-二甲基甲酰胺 作为反应溶剂,化学反应生成 5-乙炔基-2'-脱氧胞苷
参考文献:固相dna寡聚化的配有互补碱基的亚磷酰胺单体的合成.
标题:固相dna寡聚化的配有互补碱基的亚磷酰胺单体的合成.
摘要:我们描述了两个单体的制备,该单体在边缘带有互补的碱基(鸟嘌呤2'-脱氧胞苷和2-氨基腺嘌呤-2'-脱氧尿苷),并且可以方便地保护和活化以进行固相自动dna合成.我们报告了优化的合成途径,导致涉及的四个核碱基衍生物,它们交叉偶联反应成含二核碱基的单体,以及它们在dna合成仪中的低聚.
DOI:10.1021/acs.Orglett.9B03801

专利信息

专利号:EP-1600451-A2
优先权日:1999-11-12
标题 :Synthesis of 2'-deoxy-l-nucleosides

专利号:EP-1600452-A2
优先权日:1999-11-12
标题 :Synthesis of 2'-deoxy-L-nucleosides

专利号:EP-1634888-A2
优先权日:1999-11-12
标题 :Synthesis of 2'-deoxy-L-nucleosides

专利号:US-2019154696-A1
优先权日:2015-08-31
标 题 :Colorimetric Labeling and Detection Methods and Compositions
发明人：CLARKE SCOTT; GEE KYLE; FINNESSY CARMEN
权利人:LIFE TECHNOLOGIES CORP
摘要:The present disclosure provides methods, compositions and kits for measuring cellular nascent nucleic acid synthesis by colorimetric labeling of nucleic acid. The nucleic acid synthesis can be measured as cell proliferation, DNA or RNA synthesis, gene expression or apoptosis. Additionally, these methods may be used to screen compounds for their effect on cellular proliferation by treating cells or an organism with the test compound simultaneous to or before treatment with a competitive nucleoside analog.