专利号:US-4520103-A 优先权日:1982-10-27 标题:Microbial production of indigo 发明人:ENSLEY JR BURT D 权利人:AMGEN 摘要:Microbial synthesis of indigo dyestuff in indole-free media is disclosed. Indigo production is preferably accomplished by genetic transformation of selected host cells having the capacity to produce and accumulate indole (either as a result of endogenous genomic capacity or genetic transformation) to incorporate the capacity for synthesis of an aromatic dioxygenase enzyme. Growth of transformed cells under suitable conditions facilitates aromatic dioxygenase enzyme catalyzed oxidative transformation of cellular indole, with consequent formation of indigo from the oxidized reaction products. In a highly preferred embodiment, E. coli cells having endogenous indole production capacity are transformed with a DNA expression vector comprising the structural gene for naphthalene dioxygenase, resulting in the microbial synthesis of isolatable quantities of indigo.
专利号:US-9005895-B2 优先权日:2010-06-21 标题:Compositions, methods and kits for nucleic acid synthesis and amplification 发明人:WOO CORA L; BERKMAN JENNIFER; YIM PRISCILLA W 权利人:WOO CORA L; BERKMAN JENNIFER; YIM PRISCILLA W; LIFE TECHNOLOGIES CORP 摘要:The present invention is directed to compositions, methods and kits useful for the synthesis of nucleic acid molecules. More specifically, compositions, methods and kits are provided for the amplification of nucleic acid molecules in a one-step RT-PCR procedure comprising one or more agents used to increase tolerance to PCR inhibitors.
专利号:EP-4386055-A1 优先权日:2022-12-16 标题 :Sustainable process for the synthesis of bisindole alkaloids in non-conventional biodegradable solvents (deep eutectic solvents) 发明人:VITALE PAOLA; DELVECCHIO BIAGIO; PERNA FILIPPO MARIA; CICCO LUCIANA; DILAURO GIUSEPPE; CAPRIATI VITO 权利人:UNIV DEGLI STUDI DI BARI ALDO MORO; DIEFFETTI COSMETICI S R L 摘要:The present invention relates to a sustainable synthesis process for obtaining bisindole alkaloids, which uses non-conventional biodegradable solvents, such as the low melting point eutectic mixtures (Deep Eutectic Solvents, DESs) with considerable advantages both from the point of view of environmental impact and cost-effectiveness of the process.
专利号:WO-2006109424-A1 优先权日:2005-03-31 标题:Dnas encoding protein having function of forming and regulating cellulosic fiber cell wall in tree trunk and promoter dnas of the same 发明人:SATO SHIGERU; YAMADA NANAE; NAKAMOTO SHIHO; HIBINO TAKASHI 权利人:OJI PAPER CO; SATO SHIGERU; YAMADA NANAE; NAKAMOTO SHIHO; HIBINO TAKASHI 摘要:By constructing and using a eucalyptus EST database, genes participating in cell wall synthesis in a eucalyptus cellulosic fiber-forming tissue and transcriptional factors regulating the expression of these genes are extracted by the microarray analysis and differences in the expression thereof are analyzed depending on aboveground height and species. As a result, cell wall synthesis genes expressed in the eucalyptus cellulosic fiber-forming tissue and transcriptional factors are specified and it is identified that they show dominantly coordinated variation in the expression depending on aboveground height and species. Moreover, promoter DNAs of them are obtained. It is considered that the above-described genes and expression data/promoter DNAs are usable in controlling the cell wall synthesis by the cellulosic fiber-forming tissue and morphological formation.
专利号:US-2005221356-A1 优先权日:2002-09-18 标题 :Process for producing ligand binding protein with the use of cell-free protein synthesis system and utilization thereof 发明人:KIGAWA TAKANORI; MATSUDA TAKAYOSHI; AOKI MASAAKI; YOKOYAMA SHIGEYUKI; SHINOZAKI KAZUO; SEKI MOTOAKI 权利人:RIKEN 摘要:It is intended to provide a process for efficiently producing a ligand binding protein characterized in that this protein or its domain is synthesized in a cell-free protein synthesis system containing a ligand that binds to the protein or its domain. In a preferred embodiment, the above-described ligand is a substance selected from the group consisting of DNAs, RNAs, nucleosides, nucleotides, proteins, peptides, aminoacids, lipids, carbohydrates, derivatives thereof, alkaloids, terpenes, low-molecular weight compounds, coenzymes, low-molecular weight metal complexes and metal ions, while the ligand binding protein or the ligand binding domain thereof is synthesized as a soluble protein.
专利号:US-10179929-B2 优先权日:2014-06-26 标 题 :Water-soluble activatable molecular probes, intermediates for the synthesis thereof and associated detection methods 发明人:HASSERODT JENS; PROST MAXIME 权利人:ECOLE NORMALE SUPERIEURE LYON; UNIV CLAUDE BERNARD LYON; CENTRE NAT RECH SCIENT 摘要:The invention provides probes with formula (I): n nin which:n X 1 â•?NH, O, or S; X 2 â•?O or S; SE is a labile group, which may be eliminated under the action of a stimulus that may in particular be the presence of an enzyme, a chemical compound, or a physicochemical characteristic of the medium in which the probe is located; A is an aromatic group that, following cleavage of the C(X 2 )—O bond in aqueous solution, leads to the liberation of a chromophore or a fluorophore, R represents a hydrogen atom or -(L)n-GP, in which n is equal to 0 or 1, L is a linker arm, and GP is a hydrosolubilizing group; as well as their physiologically acceptable salts, solvates, or hydrates. The invention also provides intermediates for the synthesis thereof and detection methods employing them.
摘要:Joule, J. A., Science of Synthesis Knowledge Updates, (2010) 2, 1. 摘要:Chanda, A.; Fokin, V. V., Science of Synthesis: Water in Organic Synthesis, (2012) 1, 697. 摘要:S13 | EUCOSMETICS | Combined Inventory of Ingredients Employed in Cosmetic Products (2000) and Revised Inventory (2006) | DOI:10.5281/zenodo.2624118 参考文献:10.1021/jm030561b 摘要:Guengerich FP, Sorrells JL, Schmitt S, Krauser JA, Aryal P, Meijer L. Generation of new protein kinase inhibitors utilizing cytochrome p450 mutant enzymes for indigoid synthesis. J Med Chem. 2004 Jun 03;47(12):3236–41. doi: 10.1021/jm030561b.