专利号:US-2012190064-A1 优先权日:2004-10-01 标题 :Feeding buffers, systems, and methods for in vitro synthesis of biomolecules 发明人:KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA 权利人:KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA; LIFE TECHNOLOGIES CORP 摘要:Compositions, methods and kits for in vitro systems for synthesis of biomolecules such as polypeptides, are provided herein. Cell extracts that provide enhanced yields of soluble proteins using in vitro protein synthesis methods are provided. The invention also includes methods for producing high yields of proteins by the addition of a feeding solution that includes amino acids and an energy source to an ongoing in vitro synthesis system. The invention also includes methods of using a high-yield in vitro synthesis system to produce large quantities of proteins with incorporated labeled amino acids for analysis by methods such as by NMR. The invention further includes vectors for enhanced production of proteins from nucleic acid templates using in vitro synthesis systems.
专利号:US-8932545-B2 优先权日:2008-10-20 标 题:Synthesis of metal compounds 发明人:MEPSTED GARY OWEN; EWEKA EMMANUEL IMASUEN 权利人:MEPSTED GARY OWEN; EWEKA EMMANUEL IMASUEN; QINETIQ LTD 摘要:A method is provided for the synthesis of a mesoporous lithium transition metal compound, the method comprising the steps of (i) reacting a lithium salt with one or more transition metal salts in the presence of a surfactant, the surfactant being present in an amount sufficient to form a liquid crystal phase in the reaction mixture (ii) heating the reaction mixture so as to form a sol-gel and (iii) removing the surfactant to leave a mesoporous product. The mesoporous product can be an oxide, a phosphate, a borate or a silicate and optionally, an additional phosphate, borate or silicate reagent can be added at step (i). The reaction mixture can comprise an optional chelating agent and preferably, the reaction conditions at steps (i) and (ii) are controlled so as to prevent destabilization of the liquid crystal phase. The invention is particularly suitable for producing mesoporous lithium cobalt oxide and lithium iron phosphate. The method can be used to synthesize mesoporous lithium cobalt oxide having a specific surface area in excess of 10 m 2 /g, preferably 15 m 2 /g or more.
专利号:US-2002037834-A1 优先权日:2000-09-08 标题 :Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis 发明人:ASTATKE MEKBIB; GEBEYEHU GULILAT 权利人:INVITROGEN CORP 摘要:The present invention relates to cationic and polycationic compositions and methods for enhancing synthesis of nucleic acid molecules. In a preferred aspect, the invention relates to inhibition or control of nucleic acid synthesis, sequencing or amplification. Specifically, the present invention discloses cationic and polycationic molecules, compounds, and compositions having affinity for double-stranded and/or single-stranded nucleic acid molecules and/or single-stranded/double-stranded nucleic acid complexes (e.g., primer/template complexes, double-stranded templates, single-stranded templates or single-stranded primers) for use in such enhanced synthesis. The cationic and polycationic molecules, compounds, and compositions of the invention are capable of inhibiting nonspecific nucleic acid synthesis at ambient temperature. Thus, in a preferred aspect, the invention relates to “hot startâ€? synthesis of nucleic acid molecules. Accordingly, the invention prevents non-specific nucleic acid synthesis at low temperatures, for example during reaction set up. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the cationic and polycationic molecules, compounds, and compositions of the invention. The invention also relates to compositions prepared for carrying out the methods of the invention and to compositions made after or during such methods. The invention also generally relates to compositions useful for inhibiting or preventing degradation of various nucleic acid molcules.
专利号:WO-9427985-A1 优先权日:1993-05-28 标题 :Process for the preparation of substituted 3-halomethyl-benzothiophenes, benzofurans and -indoles and intermediates thereof 发明人:PRIDGEN LENDON NORWOOD 权利人:SMITHKLINE BEECHAM CORP; PRIDGEN LENDON NORWOOD 摘要:This invention relates to novel intermediates and processes for preparing useful intermediates of formula (I) which are useful in the synthesis of pharmaceutically active agents.
专利号:US-5187103-A 优先权日:1991-01-31 标题:Colorimetric method and reagent for the assay of lithium in a test sample 发明人:CZECH BRONISLAW P; CHAPOTEAU EDDY; KUMAR ANAND 权利人:MILES INC 摘要:A chromogenic cryptand found to be especially selective in the determination of lithium in blood of the general formula (I): ##STR1## where: n=1 or 2, n R 1 and R 2 , same or different, are hydrogen, lower alkyl, lower alkenyl, or lower alkylidene; and n Q is a chromogenic moiety capable of providing a detectable response upon complexation of said compound with lithium ion and has the structure: ##STR2## wherein: X is CH, C--OH or N; and Z is p-nitrophenylazo, 2,4-dinitrophenylazo, 2,4,6-trinitrophenylazo, p-nitrostyryl, p-benzoquinonemonoimino, bis-(4-dimethylaminophenyl) hydroxymethyl, 3-phenylisothiazolyl-5-azo, thiazolyl-5-azo; or isothiazolyl-5-azo. n A reagent composition and synthesis of a preferred chromogenic cryptand are also disclosed.
专利号:US-5510528-A 优先权日:1993-04-08 标题:Preparation of halomethylbenzoly cyanides and novel halomethylbenzoyl cyanides 发明人:ISAK HEINZ; WETTLING THOMAS; KEIL MICHAEL; WOLF BERND; DOETZER REINHARD 权利人:BASF AG 摘要:A process for preparing halomethylbenzoyl cyanides I n n Ph-CO-CN I n n (Ph=phenyl radical substituted by chloromethyl or bromomethyl which, if desired, can additionally carry 1-4 further radicals) by reacting halomethylbenzoyl chlorides II n n Ph-CO-Cl II n n with an alkali metal cyanide or transition metal cyanide, if appropriate in an organic diluent, and novel halomethylbenzoyl cyanides I' ##STR1## (X=halogen, C 1 -C 4 -alkyl, C 1 -C 4 -alkoxy, CF 3 , C 1 -C 5 -alkyl-(C 1 -C 5 -alkyl)hydroxyimino or C 1 -C 5 -alkyl-(C 2 -C 5 -alkenyl)hydroxyimino; m=0 to 4 and Y=chloromethyl or bromomethyl) are described. n The halomethylbenzoyl cyanides I are important intermediates for the synthesis of plant protection agents.
[参考文献]: Kahu Ss, Et Al. Two Fold Modified Chitosan For Enhanced Adsorption Of Hexavalent Chromium From Simulated Wastewater And Industrial Effluents. Carbohydr Polym. 2016 Aug 1;146:264-73. [参考文献]: Kalinich Jf, Mcclain De. Staining Of Intracellular Deposits Of Uranium In Cultured Murine Macrophages. Biotech Histochem. 2001 Sep-Nov;76(5-6):247-52. [参考文献]: Bing Wu, Et Al. Copper Oxide And Zinc Oxide Nanomaterials Act As Inhibitors Of Multidrug Resistance Transport In Sea Urchin Embryos: Their Role As Chemosensitizers. Environ Sci Technol. 2015 May 5;49(9):5760-70. [参考文献]: Mounesha N Garaga, Et Al. Local Environments Of Boron Heteroatoms In Non-Crystalline Layered Borosilicates. Phys Chem Chem Phys. 2015 Sep 7;17(33):21664-82.
合成参考文献
摘要:S13 | EUCOSMETICS | Combined Inventory of Ingredients Employed in Cosmetic Products (2000) and Revised Inventory (2006) | DOI:10.5281/zenodo.2624118