专利号:US-5824472-A 优先权日:1986-03-24 标 题 :Process for the synthesis of sugar nucleotides using recombinant-DNA methods 发明人:BETLACH MICHAEL R; DOHERTY DANIEL H; VANDERSLICE REBECCA W 权利人:MONSANTO CO 摘要:A recombinant-DNA mediated method for the synthesis of sugar nucleotides is disclosed. This method utilizes portable DNA sequences capable of directing the microbial synthesis of various enzymes that catalyze the synthesis of sugar nucleotides, including UDP-glucose, UDP-glucuronic acid and GDP-mannose. The sugar moieties of these sugar nucleotides may subsequently be incorporated into industrially-useful polysaccharides such as xanthan gum. It has been found that vectors containing the portable DNA sequences described herein are capable both of causing sugar nucleotide production in microorganisms previously incapable of such synthesis and of causing increased sugar nucleotide production in organisms capable of synthesizing small quantities of these compounds. In particular, plasmids pAS7, pAS9 and pTS13 are disclosed. These plasmids are capable of directing sugar nucleotide synthesis in various hosts, including Xanthomonas sp. such as X. campestris and other organisms such as E. coli and various Pseudomonas sp.
专利号:US-2019315790-A1 优先权日:2016-11-11 标题:Synthesis of d-allulose 发明人:KOCH TIMO JOHANNES; KIPPING FLORIAN; BUTZ STEFFEN; LESCH MARCEL; HÄSSLER THOMAS 权利人:PFEIFER & LANGEN GMBH & CO KG 摘要:The invention relates to a process for the synthesis of a product saccharide, preferably of D-allulose from an educt saccharide, preferably from D-fructose under heterogeneous or homogeneous catalysis which includes chemical and/or enzymatic catalysis. The synthesis is performed in at least two reactors that are arranged in series and the reaction product exiting the first reactor is subjected to chromatographic separation before it enters the second reactor. Preferably, the chromatographic separation is integrated in a simulated moving bed.
专利号:US-8314209-B2 优先权日:2007-12-12 标 题 :Lipid-assisted synthesis of polymer compounds and methods for their use 发明人:RAJAMANI SUDHA; OLASAGASTI FELIX; DEAMER DAVID W; BENNER SEICO 权利人:RAJAMANI SUDHA; OLASAGASTI FELIX; DEAMER DAVID W; BENNER SEICO; UNIV CALIFORNIA 摘要:The invention herein disclosed provides for methods for the synthesis of polymers from monomers. In particular the method provides for the synthesis of polynucleotides from mononucleotides in the absence of catalytic enzymes. The method comprises providing an aqueous solution having a plurality of phospholipid molecules and monomer molecules; subjecting the aqueous solution to fluctuating temperature conditions; subjecting the aqueous solution to fluctuating cycles of drying and hydrating conditions; subjecting the aqueous solution to fluctuating [H + ] conditions; the fluctuating conditions thereby allowing formation of a chemical bond between at least two monomers to create a polymer. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.
专利号:WO-2024165611-A1 优先权日:2023-02-07 标题:Production of a disaccharide and/or milk oligosaccharide by a cell with reduced synthesis of udp-glcnac 发明人:BEAUPREZ JOERI; DECOENE THOMAS; VERCAUTEREN ANNELIES 权利人:INBIOSE NV 摘要:The present invention is in the technical field of synthetic biology, metabolic engineering and cell cultivation. The invention provides a cell for production of a disaccharide and/or an oligosaccharide like a milk oligosaccharide wherein synthesis of UDP-N-acetylglucosamine in said cell is rendered less functional. The invention further provides use of said cell in a cultivation or incubation. The invention also describes methods for the production of a disaccharide and/or an oligosaccharide like a milk oligosaccharide using said cell as well as the purification of said disaccharide and/or oligosaccharide like a milk oligosaccharide.
专利号:US-12037623-B2 优先权日:2018-07-09 标 题 :Enzymatic synthesis of 4′-ethynyl nucleoside analogs 发明人:HUFFMAN MARK A; FRYSZKOWSKA ANNA; KOLEV JOSHUA N; DEVINE PAUL N; CAMPOS KEVIN R; TRUPPO MATTHEW; NAWRAT CHRISTOPHER C 权利人:MERCK SHARP & DOHME; MERCK SHARP & DOHME LLC 摘要:The present invention relates to an enzymatic synthesis of 4′-ethynyl-2′-deoxy nucleosides and analogs thereof, for example EFdA, that eliminates the use of protecting groups on the intermediates, improves the stereoselectivity of glycosylation and reduces the number of process steps needed to make said compounds. It also relates to the novel intermediates employed in the process.
专利号:WO-2024165524-A1 优先权日:2023-02-06 标 题:Production of a saccharide by a cell with reduced synthesis of lactobionic acid 发明人:BEAUPREZ JOERI; DECOENE THOMAS; VERCAUTEREN ANNELIES 权利人:INBIOSE NV 摘要:The present invention is in the technical field of synthetic biology, metabolic engineering and cell cultivation. The invention provides a cell for production of a saccharide wherein synthesis of lactobionic acid in said cell is rendered less functional or is knocked out. The invention further provides use of said cell in a cultivation or incubation. The invention also describes methods for the production of a saccharide using said cell as well as the purification of said saccharide.
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