CAS: 29393-20-2; [(2S)-2-Aminopropanoyl] (2S)-2-Amino-3-[(1R,6R)-5-Oxo-7-Oxabicyclo[4.1 .0]Hept-2-Yl]Propanoate

📜Adenosine 5'-Triphosphate,L-丙氨酸,抗荚膜菌素 反应生成 Adenosine 5'-Diphosphate,(2S)-2-{[(2S)-2-Azaniumylpropanoyl]Amino}-3-[(1R,2S,6R)-5-Oxo-7-Oxabicyclo[4.1.0]Hept-2-Yl]Propanoate,氢(+1)阳离子,H3Po4
参考文献:枯草芽孢杆菌中的ywfe编码一种新型酶l-氨基酸连接酶.
标题:枯草芽孢杆菌中的ywfe编码一种新型酶l-氨基酸连接酶.
摘要:已知atp依赖的羧酸胺/硫醇连接酶超家族包含催化各种类型的肽(例如d-丙氨酰-D-丙氨酸,聚谷氨酸和γ-肽)形成的酶,但奇怪的是,没有可合成α的酶1-氨基酸的β-二肽是已知的.我们试图找到这种酶.通过基于超家族的共有序列进行计算机筛选,然后使用纯化的酶进行体外测定以避免合成肽的降解,发现枯草芽孢杆菌的ywfe编码形成l-丙氨酰-1的活性-来自谷氨酰胺和谷氨酰胺的谷氨酰胺,Atp水解成adp.没有形成amp,支持了该酶属于超家族的观点.出人意料的是,该酶接受了各种各样的1-氨基酸.在测试的231种l-氨基酸组合中,获得了111种组合的反应产物,并通过高效液相色谱分析确定了44种α-二肽,而未检测到三肽或更长的肽,并且d-氨基酸呈惰性.根据这些结果,我们提出ywfe编码超家族的一个新成员,即i-氨基酸连接酶.
Doi:10.1128/Jb.187.15.5195-5202.2005

专利信息

专利号:US-2010279334-A1
优先权日:2007-10-31
标题 :Cyclodipeptide synthases (cdss) and their use in the synthesis of linear dipeptides
发明人：SAUGUET LUDOVIC; THAI ROBERT; BELIN PASCAL; LECOQ ALAIN; GENET ROGER; PERNODET JEAN-LUC; GONDRY MURIEL
权利人:KYOWA HAKKO BIO CO LTD; CENTRE NAT RECH SCIENT; UNIV PARIS SUD 11; COMMISSARIAT ENERGIE ATOMIQUE
摘要:Use of CDSs in the synthesis of linear dipeptides, and applications thereof for the in vivo and in vitro synthesis of linear dipeptides, in particular Phe-Leu, Leu-Phe, Phe-Phe, Phe-Tyr, Tyr-Phe, Leu-Leu, Leu-Tyr, Tyr-Leu, Phe-Met, Met-Phe, Leu-Met, Met-Leu, Tyr-Met, Met-Tyr, Met-Met, Tyr-Tyr, Ile-Met, Met-Ile, Leu-Ile, Ile-Leu using the corresponding polynucleotides.

专利号:US-7510852-B2
优先权日:2002-10-18
标题:Biosynthetic genes and host cells for the synthesis of polyketide antibiotics and method of use
发明人：ROYER MONIQUE; GABRIEL DEAN W; FRUTOS ROGER; ROTT PHILIPPE
权利人:CIRAD; UNIV FLORIDA
摘要:Three gene clusters that together encode albicidin biosynthesis, the complete gene DNA sequences, and the deduced protein sequences for the enzymes and methods for using the DNA sequences are disclosed and discussed as well as methods for plant protection and creating new antibiotics. The novel Albicidin family of antibiotics is disclosed and their structure deduced.

专利号:US-8039236-B2
优先权日:2002-12-26
标 题:Process for producing dipeptides
发明人：HASHIMOTO SHIN-ICHI; TABATA KAZUHIKO; KUBOTA AYA; IKEDA HAJIME
权利人:KYOWA HAKKO BIO CO LTD
摘要:The present invention provides a protein which catalyzes the synthesis of a dipeptide different from L-Ala-L-Ala, a process for producing the protein which catalyzes the synthesis of a dipeptide, a process for producing a dipeptide using the protein which catalyzes the synthesis of a dipeptide, and a process for producing the dipeptide using a culture of a microorganism producing the protein which catalyzes the synthesis of a dipeptide or the like as an enzyme source.

专利号:US-9968097-B2
优先权日:2012-05-30
标题:Composition comprising a biological control agent and a fungicide selected from inhibitors of the lipid membrane synthesis, the melanine biosynthesis, the nucleic acid synthesis or the signal transduction
发明人：WACHENDORFF-NEUMANN ULRIKE; ANDERSCH WOLFRAM; STENZEL KLAUS; SPRINGER BERND
权利人:BAYER CROPSCIENCE AG
摘要:The present invention relates to a composition comprising at least one biological control agent. Furthermore, the present invention relates to the use of this composition as well as a method for reducing overall damage of plants and plant parts.

专利号:WO-0003009-A3
优先权日:1998-07-10
标 题 :Genes for the biosynthesis of anticapsin and bacilysine, their isolation and their use
发明人：STEINBORN GERHARD; HOFEMEISTER JUERGEN
权利人:INST PFLANZENGENETIK & KULTUR; STEINBORN GERHARD; HOFEMEISTER JUERGEN
摘要:The invention relates to genes for the biosynthesis of anticapsin and bacilysine, their isolation and their use, notably in medicine and agriculture. These are preferably gene products which are generally suitable for use as antagonists of glucosamine synthesis and can be used as antibiosis agents against microbial pathogens and saprogens.

专利号:US-2006269988-A1
优先权日:2002-10-18
标 题 :Complete biosynthetic gene set for synthesis of polyketide antibiotics, including the albicidin family, resistance genes, and uses thereof