专利号:US-2024132927-A1 优先权日:2020-12-02 标 题 :Modular glycan production with immobilized bionanocatalysts 发明人:CORGIE STEPHANE CEDRIC; HOEPKER ALEXANDER CHRIS 权利人:ZYMTRONIX CATALYTIC SYSTEMS INC 摘要:The invention provides modular cell-free de-novo synthesis of glycans with immobilized bionanocatalysts. The invention provides materials, and in particular, magnetic materials, for producing glycans of defined length and sequences using one or more enzymes that are immobilized within bionanocatalysts (BNCs) which in turn are embedded within scaffolds to control the synthesis in batch or continuous processes manufacturing. In some embodiments, the scaffolds are high magnetism and high porosity composite blends of thermoplastics or thermosets comprising magnetic particles that form powders. In some embodiments, Selective Laser Sintering (SLS) is used to design and produce objects via 3D printing by sintering composite magnetic powders. The modular flow cells may be mixed and matched for a highly customizable and highly efficient cell-free manufacturing process. In some embodiments the elementary and system modules provided by the invention are employed. In preferred embodiments, human milk oligosaccharides (HMOs) are produced.
参考文献:10.1016/j.ceca.2004.04.001 摘要:Sehring IM, Plattner H. Ca2+ oscillations mediated by exogenous GTP in Paramecium cells: assessment of possible Ca2+ sources. Cell Calcium. 2004 Nov;36(5):409–20. doi: 10.1016/j.ceca.2004.04.001.