专利号:US-6544757-B1 优先权日:1981-08-04 标 题 :Synthesis of human virus antigens by yeast 发明人:RUTTER WILLIAM J; VALENZUELA PABLO D T; HALL BENJAMIN D; AMMERER GUSTAV 权利人:UNIV CALIFORNIA 摘要:The present invention relates to synthesis of HB S Ag in yeast. Yeast expression vectors comprising a yeast promoter, ADH1, have been constructed. The region of the HBV genome coding for the S-protein, excluding a possible 163 amino acid presequence, has been transferred to the yeast expression vector. Using the described yeast vector, the successful synthesis of HB S Ag by yeast has been achieved. The product is antigenic (reactive with anti-HB S Ag), and a substantial portion is found associated with particles identical in electron microscopic appearance to those found in the serum of HBV-infected patients and in Alexander cells but having a smaller particle size diameter. The HB S Ag synthesized by yeast has identical sedimentation behavior to purified, naturally occurring HB S Ag particles purified from Alexander cells as measured by sucrose gradient sedimentation. The present invention demonstrates synthesis and assembly of a higher ordered multi-component structure resulting from expression of a heterologous DNA coding segment in a microorganism.
专利号:EP-0072318-B1 优先权日:1981-08-04 标 题:Synthesis of human virus antigens by yeast 摘要:The present invention relates to synthesis of HBsAg in yeast. Yeast expression vectors comprising a yeast promoter, ADHI, have been constructed. The region of the HBV genome coding for the S-protein, excluding a possible 163 amino acid presequence, has been transferred to the yeast expression vector. Using the described yeast vector, the successful synthesis of HBsAg by yeast has been achieved. The product is antigenic (reactive with anti-HBsAg), and a substantial portion is found associated with particles identical in electron microscopic appearance to those found in the serum of HBV-infected patients and in Alexander cells but having a smaller particle size diameter. The HBsAg synthesized by yeast has identical sedimentation behavior to purified, naturally-occurring HBsAg particles purified from Alexander cells as measured by sucrose gradient sedimentation. The present invention demonstrates synthesis and assembly of a higher ordered multi-component structure resulting from expression of a heterologous DNA coding segment in a microorganism.
专利号:US-2019292310-A1 优先权日:2018-03-20 标 题:Synthesis of high density molecular dna brushes via organic-phase ring-opening metathesis (co)polymerization 发明人:ZHANG KE; TAN XUYU 权利人:UNIV NORTHEASTERN 摘要:The invention provides novel synthetic methods for oligonucleotide polymerization reactions that separate the deprotection and cleavage step following solid-phase oligonucleotide synthesis into two separate steps, thereby providing fully protected hydrophobic oligonucleotides that can be further manipulated in organic solvents. The disclosed methods enable the synthesis of new structures, such as brush DNA and brush RNA polymers and micellar spherical nucleic acids (SNAs
专利号:WO-2024220674-A1 优先权日:2023-04-18 标题:Timescale-guided microfluidic synthesis of polyphenol-iron iii network nanocapsules of hydrophobic drugs 发明人:YEO YOON; HAN BUMSOO; SHEN YINGNAN 权利人:PURDUE RESEARCH FOUNDATION 摘要:A scalable method of continuous microfluidic synthesis of tannic acid-iron III (TA-FeIII) network (TFN) nanocapsules of a hydrophobic drug; a scalable method of continuous microfluidic synthesis of epigallocatechin-3-gallate iron III (EGCG-FeIII) network, (EFN) nanocapsules; hydrophobic drug nanocapsules synthesized in accordance with the method; and a method of administering a hydrophobic drug to a patient in need thereof by administering to the patient the hydrophobic drug nanocapsules.
专利号:EP-0340806-B1 优先权日:1981-08-04 标 题 :Synthesis of human virus antigens by yeast 发明人:RUTTER WILLIAM J; VALENZUELA PABLO D T; HALL BENJAMIN D; AMMERER GUSTAV 权利人:UNIV CALIFORNIA; WASHINGTON RES FOUND 摘要:The present invention relates to synthesis of HBsAg in yeast. Yeast expression vectors comprising a yeast promoter, ADHl, have been constructed. The region of the HBV genome coding for the S-protein, excluding a possible 163 amino acid presequence, has been transferred to the yeast expression vector. Using the described yeast vector, the successful synthesis of HBsAg by yeast has been achieved. The product is antigenic (reactive with anti-HBsAg) , and a substantial portion is found associated with particles identical in electron microscopic appearance to those found in the serum of HBV-infected patients and in Alexander cells but having a smaller particle size diameter. The HBsAg synthesized by yeast has identical sedimentation behavior to purified, naturally-occurring HBsAg particles purified from Alexander cells as measured by sucrose gradient sedimentation. The present invention demonstrates synthesis and assembly of a higher ordered multi-component structure resulting from expression of a heterologous DNA coding segment in a microorganism.
专利号:US-7183095-B2 优先权日:2001-03-09 标题:Cell culture system for synthesis of infectious hepatitis C virus 发明人:DASGUPTA ASIM; KOKA PRASAD S 权利人:UNIV CALIFORNIA 摘要:The invention rates to a hepatitis C virus (HCV) cDNA-based culture system capable of synthesis of infectious HCV in cell culture and cell-to-cell spread of the virus. The invention also relates to a method of measuring the level of HCV infection in a hepatocyte cell. A method for identifying a modulator of HCV activity is also presented, and a method for modulating HCV activity. The invention provides a reliable system for both genetic analysis of the viral genome and for the development of novel antiviral strategies.
摘要:DHHS/ATSDR; Toxicological Profile for Uranium p. 127 (PB/99/163362) (September 1999) 摘要:BOKOVA MV et al; RENTGENOL RADIOL 14 (4): 236-40 (1975) 摘要:Lewis, R.J. Sr.; Hawley's Condensed Chemical Dictionary 14th Edition. John Wiley & Sons, Inc. New York, NY 2001., p. 1153 摘要:USEPA/Office of Water; Federal-State Toxicology and Risk Analysis Committee (FSTRAC). Summary of State and Federal Drinking Water Standards and Guidelines (11/93) To Present 摘要:Mackison, F. W., R. S. Stricoff, and L. J. Partridge, Jr. (eds.). NIOSH/OSHA - Occupational Health Guidelines for Chemical Hazards. DHHS(NIOSH) Publication No. 81-123 (3 VOLS). Washington, DC: U.S. Government Printing Office, Jan. 1981., p. 1