专利号:US-2025257380-A1 优先权日:2023-01-31 标 题:Method for template-free de novo synthesis of long-chain nucleic acid, and use thereof 发明人:XU CHENG; LIU YUANXIAO 权利人:SHANGHAI TYPING BIOSCIENCE CO LTD 摘要:The present application relates to the fields of molecular biology and biotechnology, and in particular to a method for template-free de novo synthesis of a long-chain nucleic acid, including the following steps: S1, synthesis of double-stranded oligonucleotides; and S2, combination and ligation of the double-stranded oligonucleotides to obtain a target long-chain nucleic acid. The present application achieves continuous synthesis from single nucleotides to long-chain nucleic acids by means of the combination of S1 and S2, and has the advantages of no need for templates, high accuracy, low complexity and low cost.
专利号:US-2004038331-A1 优先权日:2002-08-23 标题:Solid phase synthesis of biomolecule conjugates 发明人:REDDY M PARAMESWARA; FAROOQUI FIRDOUS; BRILLHART KURT L 摘要:Processes for the solid state phase formation synthesis of biomolecule conjugates, particularly protein-oligonucleotide conjugates are shown. One of the protein or oligonucleotide is reversibly bound to a solid substrate phase. At least one portion of each of the protein and the oligonucleotide molecules is activated with complementary activation groups. The activated protein and the activated oligonucleotide are then reacted, in a buffered solution resulting in the formation of the desired conjugate which remains reversibly bound to the substrate. The nature of the buffered solution is then modified causing the conjugate to be released from the substrate solid phase.
专利号:US-7122304-B2 优先权日:1997-05-12 标 题:Methods for the storage and synthesis of nucleic acids using a solid support 发明人:GOLDSBOROUGH MINDY D; FOX DONNA K 权利人:WHATMAN INC 摘要:The invention relates to storage of nucleic acid (particularly mRNA) on a solid support and to using such nucleic acid in nucleic acid synthesis or amplification reactions. In a preferred aspect, the invention provides synthesis of cDNA and cDNA libraries.
专利号:US-10925977-B2 优先权日:2006-10-05 标 题 :Efficient synthesis of chelators for nuclear imaging and radiotherapy: compositions and applications 发明人:YANG DAVID J; YU DONGFANG; THOMPSON ANDREW S 权利人:YANG DAVID J; YU DONGFANG; THOMPSON ANDREW S; CEIL POINT LLC; UNIV TEXAS 摘要:Novel methods of synthesis of chelator-targeting ligand conjugates, compositions comprising such conjugates, and therapeutic and diagnostic applications of such conjugates are disclosed. The compositions include chelator-targeting ligand conjugates optionally chelated to one or more metal ions. Methods of synthesizing these compositions in high purity are also presented. Also disclosed are methods of imaging, treating and diagnosing disease in a subject using these novel compositions, such as methods of imaging a tumor within a subject and methods of diagnosing myocardial ischemia.
专利号:US-2022298136-A1 优先权日:2019-08-21 标题 :Process for synthesis of pyrazolidinone compounds 发明人:MATHUR SUCHET SARAN; JAIN NANDKUMAR JANARDAN; MORE MAHENDRA MAHIPAT 权利人:GHARDA CHEMICALS LTD 摘要:A process for the synthesis of pyrazolidinone, particularly alkyl 2-(3-chloropyridin-2-yl)-5-oxo-pyrazolidine-3-carboxylate (pyrazolidinone) compound, is provided. The process is simple, economical, and produces alkyl 2-(3-chloropyridin-2-yl)-5-oxo-pyrazolidine-3-carboxylate with a comparatively high yields.
专利号:WO-2024165450-A1 优先权日:2023-02-09 标题:Method of chemical synthesis of single-chain antibody fragments and products thereby obtained 发明人:FIGINI MARIANGELA; LUISON ELENA 权利人:GLYTECH INC; FIGINI MARIANGELA 摘要:A new method is disclosed for the protein full-chemical synthesis of single-chain antibody fragments (scFv); the obtained products are structurally close and functionally equivalent to their biological counterpart, being however, advantageously free of impurities and more reproducible in properties. The method includes the general steps of: (a) separately synthetizing sub-sequences (peptide fragments) (b) sequentially assembling the sub-sequences obtained in step (a) in the order as they appear in the target scFv amino acid sequence, and (c) performing oxidative folding of the assembled whole peptide molecule obtained in step (b) and dialyzing the resulting product in a buffer.
1: Lin YL, Su YT, Chen BH. A study on inhibition mechanism of breast cancer cells by bis-type triaziquone. Eur J Pharmacol. 2010 Jul 10;637(1-3):1-10. doi: 10.1016/j.ejphar.2010.03.034. Epub 2010 Apr 2. doi: 10.3390/molecules14072306. German. German. German.
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