2-羟基-3-萘甲酸,5-氯-2-甲氧基苯胺置于三氯化磷体系中,化学反应生成色酚as-Ca
参考文献:Fluorescent Substrates For Potential Use In Enzyme-Linked Immunosorbent Assay Of Membrane-Bound Nucleic Acids
标题:Fluorescent Substrates For Potential Use In Enzyme-Linked Immunosorbent Assay Of Membrane-Bound Nucleic Acids
摘要:A Number Of Phosphorylated Fluorochromes Were Synthesized And Tested For The Alkaline Phosphatase-Linked Fluorescence Assay Of Membrane-Bound Nucleic Acids. 3-Hydroxy-N-2'-Biphenyl-2-Naphthalenecarboxamide Phosphate Ester (Hbnp) Was Found To Be The Most Suitable For The Assay. Nonfluorescent Hbnp Is Hydrolyzed By Phospholipase Bound To The Probe Dna To The Highly Fluorescent 3-Hydroxy-N,2'-Biphenyl-2-Naphthalenecarboxamide (Hbn). Hbn Is Insoluble Enough In Aqueous Medium To Precipitate On The Nylon Membrane. Spots Containing As Little As 5 Fg Of Lambda Dna Can Be Successfully Detected On The Membrane With Hbnp.
Doi:10.1021/ac00080A022