专利号:US-5139940-A 优先权日:1989-10-26 标题 :Activation compounds and methods of synthesis of activation compounds 发明人:ISAACS STEPHEN T; CIMINO GEORGE D; METCHETTE KENNETH C; TESSMAN JOHN W 权利人:ISAACS STEPHEN T; CIMINO GOERGE D; METCHETTE KENNETH C; TESSMAN JOHN W 摘要:The invention is directed to isopsoralen compound compositions that contain a variety of substitutions which yet permit their binding to nucleic acid polymers. Reaction conditions that activate these bound isopsoralens results in covalent crosslinking to the nucleic acid polymers. The resultant complex is inhibited from being a template for the template-directed enzymatic synthesis of a complementary nucleic acid polymer. Beside the isopsoralens compositions, the invention includes their synthesis, the nucleic acid complex production and detection, and the above inhibitory method of use of the isopsoralens.
专利号:US-8415510-B2 优先权日:2008-02-28 标题 :Synthesis of a PEG-6 moiety from commercial low-cost chemicals 发明人:ENGELL TORGRIM 权利人:ENGELL TORGRIM; GE HEALTHCARE LTD 摘要:The present invention provides novel synthesis's for obtaining a protecting group aminoxy PEG-6 linker from cost effective, and readily available starting materials and chemicals or modified polyethylene glycols. More specifically, a novel synthesis of obtaining a modified Boc-protected aminoxy PEG-6 linker was achieved so that said linker may be attached to a vector such as a peptide based fragment.
专利号:US-8536375-B2 优先权日:2008-12-22 标题:Synthesis of obtaining modified polyethylene glycol intermediates 发明人:ENGELL TORGRIM 权利人:ENGELL TORGRIM; GE HEALTHCARE LTD 摘要:The present invention provides novel and more efficient synthesis's for obtaining an intermediate in the synthesis of obtaining a protecting group aminoxy PEG linker.
专利号:US-6951932-B2 优先权日:2001-10-25 标 题:Synthesis of 2-aralkoxyadenosines and 2-alkoxyadenosines 发明人:MOORMAN ALLAN R 权利人:KING PHARMACEUTICALS RES & DEV 摘要:The invention provides new methods for synthesis of 2-aralkyloxyadenosines and 2-alkoxyadenosines. The invention is particularly useful for synthesis of 2-[2-(4-chlorophenyl)ethoxy]adenosine. Preferred methods of the invention include activating a guanosine compound followed by hydrolysis; alkylating the hydrolyzed compound with subsequent animation to provide a 2-aralkyloxyadenosine or a 2-alkoxyadenosine compound.
专利号:US-6384210-B1 优先权日:1997-03-20 标题:Solvent for biopolymer synthesis, solvent microdroplets and methods of use 发明人:BLANCHARD ALAN P 权利人:UNIV WASHINGTON 摘要:The present invention provides a method of biopolymer, esp. oligonucleotide, synthesis. The method consists of coupling a first nucleotide to a second nucleotide in a high surface tension solvent. The invention also provides microdroplets of a soln. comprising a solvent having a b.p. of 150 degree. C. or above, a surface tension of 30 dynes/cm or above, and a viscosity of 0.015 g/(cm)(sec), e.g., propylene carbonate. Such microdroplets are useful for the synthesis of chem. species, particularly biopolymers such as oligonucleotides and peptides, as well as arrays of chem. species. An automated system for oligonucleotide synthesis is described, which comprises delivery of microdroplets by inkjet technol. and computer control of the process. The high surface tension solvent used is selected for compatibility with the inkjet technol.
专利号:US-6646118-B2 优先权日:1996-08-30 标 题 :Solid phase synthesis 发明人:KWIATKOWSKI MAREK; NILSSON MATS; LANDEGREN ULF 权利人:QUIATECH AB 摘要:The present invention relates to a support system for solid phase synthesis of oligomers, such as oligonucleotides, wherein the starting compound is bound to the support via a disiloxyl linkage. Furthermore, the invention relates to a method for synthesis of oligonucleotides on a solid support. The support system comprises a stable disiloxyl linkage providing high nucleoside loadings to the support and the method allows convenient non-laborious oligomer synthesis.
1: Brown A, Patel S, Ward C, Lorenz A, Ortiz M, DuRoss A, Wieghardt F, Esch A, Otten EG, Heiser LM, Korolchuk VI, Sun C, Sarkar S, Sahay G. PEG-lipid micelles enable cholesterol efflux in Niemann-Pick Type C1 disease-based lysosomal storage disorder. Sci Rep. 2016 Aug 30;6:31750. doi: 10.1038/srep31750. 2: Saifer MG, Williams LD, Sobczyk MA, Michaels SJ, Sherman MR. Selectivity of binding of PEGs and PEG-like oligomers to anti-PEG antibodies induced by methoxyPEG-proteins. Mol Immunol. 2014 Feb;57(2):236-46. doi: 10.1016/j.molimm.2013.07.014. Epub 2013 Nov 5. doi: 10.1517/17425247.2012.720969. Epub 2012 Aug 30.
合成参考文献
参考文献:10.1016/j.jmb.2010.02.011 摘要:Hirano Y, Higuchi M, Azai C, Oh-Oka H, Miki K, Wang ZY. Crystal structure of the electron carrier domain of the reaction center cytochrome c(z) subunit from green photosynthetic bacterium Chlorobium tepidum. J Mol Biol. 2010 Apr 16;397(5):1175–87. doi: 10.1016/j.jmb.2010.02.011.