CAS: 146368-15-2; 1-(6-((2,5-Dioxopyrrolidin-1-yl)Oxy)-6-Oxohexyl)-2-(5-(1-(6-((2,5-Dioxopyrrolidin-1-yl)Oxy)-6-Oxohexyl)-3,3-Dimethyl-5-Sulfoindolin-2-Ylidene)Penta-1,3-Dien-1-yl)-3,3-Dimethyl-3H-Indol-1-Ium-5-Sulfonate
该化合物是一种氰化物染色,通常用于荧光标志和生物成像应用,其主要优点包括:近红外(NIR)排放强(~670纳米),高摩尔吸收性和极佳光发性,使之适合复杂生物系统敏感检测;技术等级变种优化,以便在非极端高纯度的关键工业和研究环境中以具有成本效益的方式使用;在水溶剂和有机溶剂中表现出良好的溶解性,便于与蛋白质,peptides和核酸等生物分子同化;由于生物自导纤维素干扰程度极小,5类广泛用于流动细胞测量,显微镜和活成像.
- 英文名称1-(6-((2,5-Dioxopyrrolidin-1-yl)Oxy)-6-Oxohexyl)-2-(5-(1-(6-((2,5-Dioxopyrrolidin-1-yl)Oxy)-6-Oxohexyl)-3,3-Dimethyl-5-Sulfoindolin-2-Ylidene)Penta-1,3-Dien-1-yl)-3,3-Dimethyl-3H-Indol-1-Ium-5-Sulfonate
- 中文名称花氰染料CY5
- IUPAC名称1-(6-((2,5-dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-2-(5-(1-(6-((2,5-dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-3,3-dimethyl-5-sulfoindolin-2-ylidene)penta-1,3-dien-1-yl)-3,3-dimethyl-3H-indol-1-ium-5-sulfonate
- 其它别名Cy5; 2-((1E,3E,5E)-5-(1-(5-Carboxypentyl)-3,3-Dimethylindolin-2-Ylidene)Penta-1,3-Dienyl)-1-Ethyl-3,3-Dimethyl-3H-Indolium Chloride; 1-[6-[(2,5-Dioxo-1-Pyrrolidinyl)Oxy]-6-Oxohexyl]-2-[5-[1-[6-[(2,5-Dioxo-1-Pyrrolidinyl)Oxy]-6-Oxohexyl]-1,3-Dihydro-3,3-Dimethyl-5-Sulfo-2H-Indol-2-Ylidene]-1,3-Pentadien-1-Yl]-3,3-Dimethyl-5-Sulfo-3H-Indolium Inner Salt; Cy5 Dise(Di So3); Sulfo-Cy5 Diacid, Se; Cy 5 (Technical Grade); 3H-Indolium,1-[6-[(2,5-Dioxo-1-Pyrrolidinyl)Oxy]-6-Oxohexyl]-2-[5-[1-[6-[(2,5-Dioxo-1-Pyrrolidinyl)Oxy]-6-Oxohexyl]-1,3-Dihydro-3,3-Dimethyl-5-Sulfo-2H-Indol-2-Ylidene]-1,3-Pentadienyl]-3,3-Dimethyl-5-Sulfo-, Inner Salt
- CAS编号146368-15-2
- MFCD编号:MFCD28978196
- FDA UNII编号:HSK439WFK4
- 分子式:C45H52N4O14S2分子量:937.04
- 产品CID: 697166
- 产品分类生物 → 蛋白与多肽 → 蛋白质免疫印迹 (WB)
合成工艺路线路线简述
- 合成目标产物 Cy5 主要起始原料 N-Hydroxysuccinimide And Cyanine 5 Bisacid [equivalent To Cy5 Bisacid]
- (文献来源)合成步骤主要原料 N-Hydroxysuccinimide 和 Cyanine 5 Bisacid [equivalent To Cy5 Bisacid]
📜N-羟基丁二酰亚胺,Cy5,双酸置于n,N'-二环己基碳二亚胺体系中,用 N,N-二甲基甲酰胺 用作溶剂,化学反应 10.0H,反应生成花氰染料cy5
参考文献:Novel Asymmetric Cy5 Dyes: Synthesis,Photostabilities And High Sensitivity In Protein Fluorescence Labeling
标题:Novel Asymmetric Cy5 Dyes: Synthesis,Photostabilities And High Sensitivity In Protein Fluorescence Labeling
摘要:Several Novel Water-Soluble Asymmetric Pentamethine Cyanine Dyes Were Synthesized. The Maximum Absorption And Emission Wavelengths Of The Dyes In Different Solvents Were In The Range From 647 To 665 Nm And Exhibited Negative Solvatochromism With Increasing Solvent Polarity. The Fluorescence Quantum Yields Of The Dyes Were About 0.1 In Water,And Were Obviously Higher Than Those Of Hydrophobic Cyanine Dyes. Dyes With N-Benzyl Groups And N-Sulfo-Groups Displayed Greater Photostability Than Dyes With N-Carboxypentyl Groups In Water. The Limit Of Detection Of Dye 5A For Bsa Was 1.2 X 10(-8) Mol L-1 By High Performance Liquid Chromatography With Fluorescent Director About 100-Fold Lower Than That By Uv Detection (1.0 X 10(-6) Mol L-1). Therefore,Dye 5A Could Be Used To Improve Photostability And Detection Sensitivity In Protein Analysis. (C) 2010 Elsevier B.V. All Rights Reserved.
Doi:10.1016/j.Jphotochem.2010.01.002
