CAS: 13446-24-7; Magnesium Diphosphate

专利信息

专利号:WO-9640901-A1
优先权日:1995-06-07
标题 :Template and primer based synthesis of enzymatically cleavable oligonucleotides
发明人：DATTAGUPTA NANIBHUSHAN; KACIAN DANIEL LOUIS; LACKEY DAVID BRUCE
权利人:GEN PROBE INC
摘要:The present invention relates to methods of enzymatically synthesizing chirally pure oligonucleotides. In particular, it relates to methods of enzymatically synthesizing oligonucleotides using optionally digestible templates which are capable of directing the synthesis of an oligonucleotide product which can optionally be cleaved into two or more individual oligonucleotides, optionally using 3'-ribonucleotide primers. In this manner, more than one oligonucleotide having the same or different length and/or sequence can be simultaneously synthesized. The oligonucleotides optionally have at least one phosphorothioate linkage, and contain certain restriction endonuclease recognition sites herein identified as cleavable within phosphorothioate oligonucleotides, thus facilitating the separation and purification of the oligonucleotides after synthesis.

专利号:US-5436143-A
优先权日:1992-12-23
标题:Method for enzymatic synthesis of oligonucleotides
发明人：HYMAN EDWARD D
摘要:Enzymatic synthesis of oligonucleotides may be performed in a single vessel without intermediate purification, by the steps of: n (a) combining a nucleotide primer sequence and a blocked nucleotide in the presence of a chain extending enzyme whereby a reaction mixture is formed containing the blocked nucleotide coupled to the nucleotide primer sequence at its 3' end; n (b) inactivating the chain extending enzyme; n (c) removing the blocking group from the primer-blocked nucleotide to form a primer-nucleotide product; and converting any unreacted blocked nucleotide to an unreactive form which is substantially less active as a substrate for the chain extending enzyme than the blocked nucleotide.

专利号:US-4666945-A
优先权日:1983-11-14
标 题:Catalyst composition suitable for synthesis of methanol
发明人：OSUGI MINORU; TAKAGAWA MAKOTO; NAKAMURA TADASI; KOJIMA TAKASHI; TSUJI KINYA
权利人:MITSUBISHI GAS CHEMICAL CO
摘要:A catalyst composition comprising copper oxide, zinc oxide and zirconium oxide, the content of zirconium oxide being 30 to 70% by weight. This catalyst composition is useful for synthesis of methanol from carbon monoxide and/or carbon dioxide and hydrogen by a gas-liquid-solid phase fluidized bed method or a gas-solid phase fluidized bed method.

专利号:US-5916777-A
优先权日:1995-06-07
标 题:Enzymatic synthesis of oligonucleotides using 3'-ribonucleotide primers
发明人：KACIAN DANIEL LOUIS; DATTAGUPTA NANIBHUSHAN; LACKEY DAVID BRUCE
权利人:GEN PROBE INC
摘要:The present invention relates to methods of enzymatically synthesizing oligonucleotides. In particular, it relates to the use of 3'-ribonucleotide primers which effectively serve to initiate oligonucleotide synthesis, and can also be easily cleaved from synthesis products and reused in subsequent synthesis reactions.

专利号:US-5932450-A
优先权日:1995-06-07
标题 :Enzymatic synthesis of oligonucleotides using digestible templates
发明人：DATTAGUPTA NANIBHUSHAN; KACIAN DANIEL L; LACKEY DAVID BRUCE
权利人:GEN PROBE INC
摘要:The present invention describes methods of synthesizing oligonucleotides. In particular, it relates to methods of enzymatically synthesizing chirally pure oligonucleotides using templates which can be digested after synthesis. By using digestible templates, separation and purification of the synthesized oligonucleotides are greatly facilitated.

专利号:US-5739311-A
优先权日:1995-06-07
标题:Enzymatic synthesis of phosphorothioate oligonucleotides using restriction endonucleases
发明人：LACKEY DAVID BRUCE; DATTAGUPTA NANIBHUSHAN; KACIAN DANIEL LOUIS
权利人:GEN PROBE INC
摘要:The present invention relates to methods of synthesizing phosphorothioate oligonucleotides. In particular, it relates to the use of certain restriction endonucleases to cleave phosphorothioate oligonucleotides which contain restriction endonuclease recognition sequences. These restriction sequences facilitate the cleavage of relatively cleavage resistant phosphorothioate oligonucleotides thus facilitating their separation and purification after synthesis.