专利号:US-11001668-B2 优先权日:2017-02-10 标题 :Synthesis of polyglycolic acid with high molecular weight and high solubility at low cost 发明人:SEZER SERDAR; AYDEMIR SEZER UMRAN; SANKO VILDAN 权利人:TUBITAK 摘要:The invention relates to the synthesis method of polyglycolic acid (PGA) obtained by removing the excessive water and monomer formed subsequent to condensation by means of an organic solvent and using azeotropic distillation method in the presence of a catalyst. The invention is a synthesis method of polyglycolic acid with high molecular weight and high solubility, characterized by comprising the steps of placing the glycolic acid in reaction medium with a catalyst; using hydrophilic organic solvents for removing the excessive water and monomer formed during condensation; mixing and boiling the mixture; stabilizing the amount of solvent in reaction medium and distilling the water off by means of Dean stark apparatus and/or a similar apparatus; refluxing the solid polymer obtained at the end of the reaction with ethyl acetate and removing the same from monomer residue and catalyst.
专利号:US-2004014180-A1 优先权日:2000-09-14 标 题 :Method for the microbial production of metabolic products, polynucleotides from coryneform bacteria and use thereof 发明人:BOTT MICHAEL; NIEBISCH AXEL; BATHE BRIGITTE; MARX ACHIM; HERMANN THOMAS 摘要:The invention relates to a method for the microbial production of metabolic products, polynucleotides from coryne-form bacteria and use thereof. According to the invention, by means of said method and polynucleotides it is possible to influence the synthesis of ATP in a controlled manner and also to control the synthesis of metabolic products. The invention relates to genes from Corynebacterium glutamicum coding for cytochrome aa 3 oxidase and the cytochrome bc 1 complex. The monocistronic ctaD gene codes for a 65 kDa protein, the primary structure of which displays all the typical properties of the sub-unit I of cytochrome aa 3 oxidase. The genes which code for the sub-unit III of the cytochrome aa 3 (ctaE) and the three characteristic sub-units of the cytochrome bc 1 complex (qcrABC) are arranged in a group with the sequence ctaE-qcrCAB. An analysis of the derived primary structure shows a sequence with unusual properties: (i) cytochrome C 1 (QcrC, 30 kDa) contains two Cys-X—X-Cys-His groups for the covalent bonding of haeme, which means that said protein is a di-haeme cytochrome of the c type; (ii) the “Rieskeâ€? iron-sulphur protein (QcrA, 45 kDa) presumably contains three trans-membrane helices in the N-terminal region; (iii) cytochromeb (QcrB, 60 kDa) contains a C-terminal extension of 120 amino acids, along with the conserved region with 8 trans-membrane helices, presumably localised in the cytoplasma. The electron transfer from the cytochrome bc 1 complex to the cytochrome aa 3 terminal oxidase does not involve additional ctochrome c.
专利号:US-9169502-B2 优先权日:2010-06-15 标题 :Method of producing L-lysine using a Corynebacterium glutamicum microorganism 发明人:WITTMANN CHRISTOPH; BECKER JUDITH 权利人:WITTMANN CHRISTOPH; BECKER JUDITH; PAIK KWANG IND CO LTD 摘要:The present invention is directed to a method utilizing a recombinant microorganism for the production of aspartate derived amino acids and precursors thereof, in particular for the production of L-lysine. Furthermore, the present invention relates to a recombinant microorganism having improved aspartate-derived amino acid synthesis activity in comparison to the initial microorganism and the use of such microorganisms in producing said amino acids and precursors and derivatives, in particular in the synthesis of L-lysine.
专利号:WO-2014028026-A9 优先权日:2012-08-17 标题:Biological synthesis of difunctional hexanes and pentanes from carbohydrate feedstocks 发明人:BAYNES BRIAN M; GEREMIA JOHN MICHAEL; LIPPOW SHAUN 权利人:CELEXION LLC; BAYNES BRIAN M; GEREMIA JOHN MICHAEL; LIPPOW SHAUN 摘要:Provided herein are methods for the production of difunctional alkanes in microorganisms. Also provided are enzymes and nucleic acids encoding such enzymes, associated with the difunctional alkane production from carbohydrates feedstocks in microorganisms. The invention also provides recombinant microorganisms and metabolic pathways for the production of difunctional alkanes.
专利号:US-11560575-B2 优先权日:2017-12-29 标 题:High efficient delivery of plasmid DNA into human and vertebrate primary cells in vitro and in vivo by nanocomplexes 发明人:DOU HUANYU 权利人:UNIV TEXAS TECH SYSTEM 摘要:The present invention includes synthesis of polyethyleneimine800-EpoxyC8-22 (PEI800-C8-22) lipids, e.g., Polyethyleneimine800-EpoxyC16 (PEI800-C16), PEI12C16, PEI8C16, and PEI4C16 lipids, compositions and methods for transfecting primary leukocytes, myeloid cells, lymphoid cells, monocytes, macrophages and dendritic cells (DC) comprising a transfection complex comprising: one or more nanoparticles; and Polyethyleneimine800-EpoxyC16 (PEI800-C16), PEI12C16, PEI8C16, and PEI4C16 lipids complexed with one or more nucleic acids, such as, e.g., DNA, RNA, nucleic acid vectors, shRNA, miRNA, and RNAi on or about the nanoparticles.
专利号:US-9102961-B2 优先权日:2009-03-11 标 题 :Biological synthesis of difunctional hexanes and pentanes from carbohydrate feedstocks 发明人:BAYNES BRIAN M; GEREMIA JOHN MICHAEL; LIPPOW SHAUN M 权利人:CELEXION LLC 摘要:Provided herein are methods for the production of difunctional alkanes in microorganisms. Also provided are enzymes and nucleic acids encoding such enzymes, associated with the difunctional alkane production from carbohydrates feedstocks in microorganisms. The invention also provides recombinant microorganisms and metabolic pathways for the production of difunctional alkanes.
1: Wei W, Lau WB, Chen Y, Hu X, Nie H. Severe lactic acidosis and acute hepatic and renal insufficiency due to fenaminosulf poisoning. Clin Toxicol (Phila). 2014 Apr;52(4):446-7. doi: 10.3109/15563650.2014.900181. Epub 2014 Mar 20. doi: 10.1016/j.bios.2015.04.066. Epub 2015 Apr 21. 6: Wang LC, Yu YQ, Fang M, Zhan CG, Pan HY, Wu YN, Gong ZY. Antioxidant and antigenotoxic activity of bioactive extracts from corn tassel. J Huazhong Univ Sci Technolog Med Sci. 2014 Feb;34(1):131-6. doi: 10.1007/s11596-014-1244-x. Epub 2014 Feb 6. Chinese. French. German.
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