其它别名4,4''-Triaminotriphenylmethan-Hydrochlorid; C.I. Basic Red 9 (Van); C.I. Basic Red 9 Monohydrochloride; Ci Basic Red 9, Monohydrochloride; Fuchsine Spc; Parasaniline; Benzenamine,5-Cyclohexadien-1-Ylidene)Methyl],Monohydr
专利号:US-7541165-B2 优先权日:2001-10-30 标 题 :Molecular detection systems utilizing reiterative oligonucleotide synthesis 发明人:HANNA MICHELLE M 权利人:RIBOMED BIOTECHNOLOGIES INC 摘要:The present invention provides methods for detecting the presence of a target molecule by generating multiple detectable oligonucleotides through reiterative enzymatic oligonucleotide synthesis events on a defined polynucleotide sequence. The methods generally comprise using a nucleoside, a mononucleotide, an oligonucleotide, or a polynucleotide, or analog thereof, to initiate synthesis of an oligonucleotide product that is substantially complementary to a target site on the defined polynucleotide sequence; optionally using nucleotides or nucleotide analogs as oligonucleotide chain elongators; using a chain terminator to terminate the polymerization reaction; and detecting multiple oligonucleotide products that have been synthesized by the polymerase. In one aspect, the invention provides a method for detecting a target protein, DNA or RNA by generating multiple detectable RNA oligoribonucleotides by abortive transcription.
专利号:WO-9102067-A1 优先权日:1989-07-27 标题:Regulation of nerve growth factor synthesis in the central nervous system 发明人:LINDHOLM DAN BJARNE; THOENEN HANS FRIEDRICH ERWIN; HENGERER BASTIAN 权利人:MAX PLANCK GESELLSCHAFT 摘要:The present invention relates to a method for regulating levels of nerve growth factor in the central nervous system, and is based on the discovery that in vivo synthesis of nerve growth factor may be regulated by various cytokines. In specific embodiments, intracerebroventricular injection of interleukin 1, fibroblast growth factor, or TGFβ1 increase synthesis of nerve growth factor and its mRNA. The methods of the present invention may be useful in the treatment of various neurologic diseases or disorders, including Alzheimer's disease.
专利号:US-2004054162-A1 优先权日:2001-10-30 标 题:Molecular detection systems utilizing reiterative oligonucleotide synthesis 发明人:HANNA MICHELLE M 摘要:The present invention provides methods for detecting the presence of a target molecule by the use of nucleotide analogs containing moieties that enable detection. Such analogs may be incorporated into nucleic acids. In one embodiment, nucleotide analogs are used in a process generating multiple detectable oligonucleotides through reiterative enzymatic oligonucleotide synthesis events on a defined polynucleotide sequence. The methods generally comprise using a nucleoside, a mononucleotide, an oligonucleotide, or a polynucleotide, or analog thereof, to initiate synthesis of an oligonucleotide product that is substantially complementary to a target site on the defined polynucleotide sequence; optionally using nucleotides or nucleotide analogs as oligonucleotide chain elongators or chain terminators to terminate the polymerization reaction; and detecting multiple oligonucleotide products that have been synthesized by the polymerase.
专利号:US-2014378538-A1 优先权日:2011-12-14 标 题:Methods of responding to a biothreat 发明人:BANCEL STEPHANE 权利人:MODERMA THERAPEUTICS INC 摘要:The present disclosure provides for devices, in particular mobile devices, which may be used in the synthesis of modified nucleic acid molecules, in particular modified mRNA molecules. The device for making the modified nucleosides, modified nucleotides and modified nucleic acids (e.g., mRNA) disclosed herein may be mobile devices comprising at least one sample block for insertion of one or more sample vessels, a device base with electronic control units for the sample block, a voltage supply, and one or more reagent(s) for the synthesis of at least one nucleic acid.
专利号:US-2022233673-A1 优先权日:2019-06-04 标 题:METHODS OF PRODUCING SHIGA TOXIN B-SUBUNIT (STxB) MONOMERS AND OLIGOMERS, AND USES THEREOF 发明人:BILLET ANNE; SCHMIDT FRÉDÉRIC; JOHANNES LUDGER; SERVENT DENIS; MOURIER GILLES; TARTOUR ÉRIC; KAY MICHAEL; FULCHER JAMES M 权利人:INST CURIE; CENTRE NAT RECH SCIENT; INST NAT SANTE RECH MED; COMMISSARIAT A LENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES CEA; APHP ASSIST PUBLIQUE HOPITAUX DE PARIS; UNIV PARIS; UNIV OF UTAH RESEARCH FOUDATION; UNIV UTAH RES FOUND 摘要:A method of producing a monomer of a Shiga toxin B-subunit (STxB) protein or of a variant thereof by peptide chemical synthesis, as well as to a method of producing a pentamer of the STxB protein or of the variant thereof. The methods are particularly advantageous as they overcome major issues typically observed in peptide chemical synthesis, including solubility and purity issues.
专利号:US-9845501-B2 优先权日:1998-12-14 标题 :System and methods for nucleic acid sequencing of single molecules by polymerase synthesis 发明人:WILLIAMS JOHN G K 权利人:PACIFIC BIOSCIENCES CALIFORNIA INC; PACIFIC OF BIOSCIENCES OF CALIFORNIA INC 摘要:This invention relates to improved methods for sequencing and genotyping nucleic acid in a single molecule configuration. The method involves single molecule detection of fluorescent labeled PPi moieties released from NTPs as a polymerase extension product is created.
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合成参考文献
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