CAS: 2321-07-5; 3',6'-Dihydroxy-3H-Spiro[isobenzofuran-1,9'-Xanthen]-3-One

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CAS号596-09-8 二乙酸荧光素 | CAS号85-44-9 苯酐 | CAS号108-46-3 间苯二酚 | CAS号3326-31-6 6-异硫氰酸荧光素 | CAS号3326-34-9 5-氨基荧光素 | CAS号108-95-2 苯酚 | CAS号89-84-9 2',4'-二羟基苯乙酮 | CAS号83616-10-8 FLUORESCEIN MON... | CAS号3326-32-7 异硫氰酸荧光素酯 | CAS号36886-76-7 3',6'-二甲氧基荧烷 | CAS号2861-41-8 2',4',5'-trichl... | CAS号2320-38-9 2',4',5',7'-tet... | CAS号24545-86-6 4 ,5-二硝基荧光素 | CAS号25709-83-5 Spiroisobenzofu... | CAS号2513-33-9 2',4'-二羟基-2-苯甲酰基苯甲酸 | CAS号108-46-3 间苯二酚 | CAS号65-85-0 苯甲酸

Fluorescein Free Acid 以 水,二甲基亚砜 用作溶剂,化学反应生成荧光素
参考文献:Extraordinary Character Of The Solvent Influence On Protolytic Equilibria: Inversion Of The Fluorescein Ionization Constants In H2O-dmso Mixtures
标题:Extraordinary Character Of The Solvent Influence On Protolytic Equilibria: Inversion Of The Fluorescein Ionization Constants In H2O-dmso Mixtures
摘要:采用分光光度法研究了h2O-Dmso体系中荧光素和磺基荧光素的质子分解平衡.确定染料的各个离子(分子)形式的 Pka 值以及吸收光谱. Pka 计算中使用的 Pa*h 尺度 [dmso 含量 (Wt.%) 为 21.6,42.2,60.3,81.4 和 91.3] 通过指示剂法(磺酞系列)通过重叠程序进行估计.溶剂影响的特征荧光素逐步电离(h3R+->h2R->hr->r2->;分别为 Kao,Ka1 和 Ka2)导致 Ka 常数之间存在非同寻常的相互关系,直至反转:Pka1 > Pka2. Ka1/ka2 比值逐渐从 H2O 中的 224 变为 91% Dmso 中的 0.045;同时,Ka0/ka1 比率从 204 增加到 7 x 1010.荧光素 Pka 依赖于 Dmso 含量(最高 Pka1 > Pka2)的异常特征可以通过互变异构平衡位移和官能团的性质来解释.荧光素中性形式的互变异构常数是根据先前提出的热力学假设获得的(n. O. Mchedlov-Petrossyan,Zh. Anal. Khim.,1979,34,1055).这使得可以计算电离微常数 (K),发现该常数与模型化合物的 Pka 值一致.
Doi:10.1039/ft9928803025

专利信息

专利号:US-9365607-B1
优先权日:2012-07-31
标题 :Synthesis of deuterated ribo nucleosides, N-protected phosphoramidites, and oligonucleotides
发明人：SRIVASTAVA SURESH C; YASKO AMY
权利人:ASED LLC
摘要:The present invention is directed towards the synthesis of high purity deuterated sugars, deuterated phosphoramidites, deuterated nucleobases, deuterated nucleosides, deuterated oligonucleotides, and deuterated RNA's of defined sequences which can exhibit biochemically useful and biologically valuable properties, thus having potential for therapeutic uses.

专利号:US-5777133-A
优先权日:1987-12-31
标 题:Synthesis of 1, 2-dioxetanes and intermediates therefor
发明人：BRONSTEIN IRENA Y; EDWARDS BROOKS
权利人:TROPIX INC
摘要:Compounds having the formula: ##STR1## wherein T is a polycycloalkylidene group (e.g., adamant-2-ylidene); R is a C 1-20 alkyl, aralkyl or cycloalkyl group; and Y is a fluorescent chromophore (eg., m-phenylene), produced by reacting a compound having the formula: ##STR2## with an R-ylating agent (e.g., R 2 SO 4 ) in the presence of an alkali metal alkoxide in a polar aprotic solvent. Also, compounds having the formula: ##STR3## are produced by reacting a compound having the formula: ##STR4## wherein X is an electronegative leaving group (e.g., a halogen anion such as chloride ion) in the presence of a Lewis base (e.g., a trialkyl-amine) dissolved in an aprotic organic solvent (e.g., benzene or toluene). Also, compounds having the formula ##STR5## are produced by reacting a compound of the formula ##STR6## with a tetra-O-acylated-O-hexopyranoside halide, then hydrolyzing off the protective acyl groups. The aforementioned compounds and procedures are useful in the synthesis of enzyme-cleavable 1,2-dioxetane ring systems that can serve as members of a binding pair employed, for example, in chemiluminescent immunoassays, DNA probe assays, and direct assays for an enzyme.

专利号:US-2007049745-A1
优先权日:2005-07-11
标题 :Oligonucleotide synthesis using periodate salts
发明人：SKRZYPCZYNSKI ZBIGNIEV
权利人:THIRD WAVE TECH INC
摘要:The present invention relates generally to nucleic acid chemistry and to the chemical synthesis of oligonucleotides. More particularly, the invention relates to improved methods for synthesizing oligonucleotides wherein periodate salts are used (e.g., in organic solvents) as an oxidation reagent in oligonucleotide synthesis (e.g., for automated phosphoramidite synthesis of oligonucleotides). The invention finds utility in the fields of biochemistry, molecular biology, and pharmacology, and in medical diagnostic and screening technologies.

专利号:US-7041446-B2
优先权日:1998-05-15
标 题 :Labeled protein and its producing method, labeling compound to be used in the method, and method for analyzing function of genes
发明人：YANAGAWA HIROSHI; NEMOTO NAOTO; MIYAMOTO ETSUKO
权利人:MITSUBISHI CHEM CORP
摘要:A protein having a labeling compound attached to its C-terminal, in which the compound comprises a label portion comprising a label substance and an acceptor portion comprising a compound having an ability of binding to a C-terminal of a synthesized protein when protein synthesis is carried out in a cell-free protein synthesis system or in a living cell is provided. Also, a method for producing the protein comprising the step of performing synthesis of a protein in a cell-free protein synthesis system or in a living cell in the presence of a labeling compound comprising a label portion comprising a label substance and an acceptor portion comprising a compound having an ability of binding to a C-terminal of a synthesized protein when protein synthesis is carried out in the cell-free protein synthesis system or in the living cell, the labeling compound being present at a concentration effective for the labeling compound to bind to the C-terminal of the synthesized protein is provided. Also, the labeling compound and a method for analyzing a function of a gene are further provided.

专利号:WO-0012554-A1
优先权日:1998-08-28
标题:Method for marked chemokine synthesis, marked chemokine and analysis kit
发明人：VITA CLAUDIO; VIZZAVONA JEAN; GLUCKMAN JEAN CLAUDE; BENJOUARD ABDELAZIZ
权利人:COMMISSARIAT ENERGIE ATOMIQUE; UNIV PARIS CURIE; VITA CLAUDIO; VIZZAVONA JEAN; GLUCKMAN JEAN CLAUDE; BENJOUARD ABDELAZIZ
摘要:The invention concerns a method for the homogeneous synthesis of a marked chemokine in a specific position. The invention also concerns a chemokine obtained by said method, a composition and a kit comprising said chemokine. The invention is characterised in the method for the synthesis of the marked chemokine comprises a step which consists in a solid phase chemical synthesis of the chemokine or a modified sequence thereof.

专利号:US-7541165-B2
优先权日:2001-10-30
标 题 :Molecular detection systems utilizing reiterative oligonucleotide synthesis
发明人：HANNA MICHELLE M
权利人:RIBOMED BIOTECHNOLOGIES INC
摘要:The present invention provides methods for detecting the presence of a target molecule by generating multiple detectable oligonucleotides through reiterative enzymatic oligonucleotide synthesis events on a defined polynucleotide sequence. The methods generally comprise using a nucleoside, a mononucleotide, an oligonucleotide, or a polynucleotide, or analog thereof, to initiate synthesis of an oligonucleotide product that is substantially complementary to a target site on the defined polynucleotide sequence; optionally using nucleotides or nucleotide analogs as oligonucleotide chain elongators; using a chain terminator to terminate the polymerization reaction; and detecting multiple oligonucleotide products that have been synthesized by the polymerase. In one aspect, the invention provides a method for detecting a target protein, DNA or RNA by generating multiple detectable RNA oligoribonucleotides by abortive transcription.