专利号:US-7344863-B2 优先权日:1998-03-13 标题:Methods for producing polypeptides through enhanced synthesis of encoding nucleic acid molecules 发明人:LI WU-BO; JESSEE JOEL A; SCHUSTER DAVID; XIA JIULIN; GEBEYEHU GULILAT 权利人:INVITROGEN CORP 摘要:The present invention is directed to compositions and methods for enhancing synthesis of nucleic acid molecules, particularly GC-rich nucleic acid molecules. Specifically, the invention provides compositions comprising one or more nitrogen-containing organic compounds having a formula selected from the group consisting of formula I and formula II (or salts or derivatives thereof), preferably 4-methylmorpholine N-oxide or betaine (carboxymethyltrimethylammonium), and further comprising one or more compounds selected from the group consisting of proline and an N-alkylimidazole compound, and more preferably proline, 1-methylimidazole or 4-methylimidazole. The invention further relates to methods for enhanced, high-fidelity synthesis of nucleic acid molecules, including via amplification (particularly PCR), reverse transcription, and sequencing methods. The invention also relates to nucleic acid molecules synthesized by these methods, to fragments or derivatives thereof, and to vectors and host cells comprising such nucleic acid molecules, fragments, or derivatives. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the compositions of the invention.
专利号:US-9034844-B2 优先权日:2009-04-06 标 题 :6′-sialyllactose salts and process for their synthesis and for the synthesis of other alpha-sialyloligosaccharides 发明人:TAMERLANI GIANCARLO; LOMBARDI ILARIA; BARTALUCCI DEBORA; DANESI ANDREA; SALSINI LIANA; MANONI MARCO; CIPOLLETTI GIOVANNI 权利人:TAMERLANI GIANCARLO; LOMBARDI ILARIA; BARTALUCCI DEBORA; DANESI ANDREA; SALSINI LIANA; MANONI MARCO; CIPOLLETTI GIOVANNI; INALCO SPA 摘要:The present invention relates to a process of synthesis of α-sialyl oligosaccharides and in particular of 6′-sialyllactose and its salts comprising a step of coupling by Koenigs-Knorr reaction under conditions that allow its use on an industrial scale.
专利号:US-2024024489-A1 优先权日:2020-11-20 标 题:Protected disaccharides, their process of preparation and their use in the synthesis of zwitterionic oligosaccharides, and conjugates thereof 发明人:MULARD LAURENCE; DHARA DEBASHIS; PFISTER HELENE; PAOLETTI JULIE; PHALIPON ARMELLE; GUERREIRO-INVERNO CATHERINE 权利人:PASTEUR INSTITUT 摘要:The present invention provides zwitterionic oligosaccharides, in particular fragments of the surface polysaccharides from Shigella sonnei and Shigella sonnei conjugates comprising them. The present invention also provides protected disaccharides, their process of preparation and their use in the synthesis of zwitterionic oligosaccharides, and conjugates thereof, the disaccharide repeating unit of Shigella sonnei being: (I)
专利号:US-7429658-B2 优先权日:2003-09-11 标题 :Synthesis of protected pyrrolobenzodiazepines 发明人:HOWARD PHILIP; MASTERSON LUKE 权利人:SPIROGEN LTD 摘要:A method of synthesis of a N-10 protected PBD compound of formula (I): n nvia an intermediate of formula (II) or formula (V):
专利号:US-10973847-B2 优先权日:2017-06-30 标题 :Core-to-surface polymerization for the synthesis of star polymers and uses thereof 发明人:JOHNSON JEREMIAH A; GOLDER MATTHEW R 权利人:MASSACHUSETTS INST TECHNOLOGY 摘要:Disclosed are methods, compositions, reagents, systems, and kits to prepare star polymers, as well as compositions and uses thereof. Various embodiments show that synthesis of these polymers contain low metal concentration to provide polymers for diverse biomedical applications including in vivo applications.
专利号:US-8183355-B2 优先权日:2006-04-28 标题 :Method for the synthesis of oligonucleotide derivatives 发明人:MORVAN FRANCOIS; MEYER ALBERT; VASSEUR JEAN-JACQUES; VIDAL SEBASTIEN; CLOAREC JEAN-PIERRE; CHEVOLOT YANN; SOUTEYRAND ELIANE 权利人:MORVAN FRANCOIS; MEYER ALBERT; VASSEUR JEAN-JACQUES; VIDAL SEBASTIEN; CLOAREC JEAN-PIERRE; CHEVOLOT YANN; SOUTEYRAND ELIANE; CENTRE NAT RECH SCIENT 摘要:Method for the synthesis of nucleotide derivatives wherein molecules of interest are grafted on the oligonucleotide with the help of a “click chemistryâ€? reaction between an azide function on the molecule of interest and an alkyne function on the oligonucleotide, or between an alkyne function on the molecule of interest and an azide function on the oligonucleotide. n Intermediate molecules, notably alkyne functionalized oligonucleotides, grafted oligonucleotides, azide functionalized oligonucleotides, oligonucleotide micro arrays containing them and the use of those grafted oligonucleotides for biological investigation and for cell targeting.
参考标题:Two-Step Synthesis Of Per-O-Acetylfuranoses: Optimization And Rationalization 作者:Rémy Dureau,Laurent Legentil,Richard Daniellou,Vincent Ferrières |发布日期:2012.2.3 摘要:A Simple Two-Step Procedure Yielding Peracetylated Furanoses Directly From Free Aldoses Was Implemented. Key Steps Of The Method Are (I) Highly Selective Formation Of Per-O-(Tert-Butyldimethylsilyl)Furanoses And (II) Their Clean Conversion Into Acetyl Ones Without Isomerization. This Approach Was Easily Applied To Galactose And Structurally Related Carbohydrates Such As Arabinose, Fucose, Methyl Galacturonate
合成参考文献
参考文献:10.1007/s00253-018-9018-1 摘要:Vergne-Vaxelaire C, Mariage A, Petit JL, Fossey-Jouenne A, Guérard-Hélaine C, Darii E, Debard A, Nepert S, Pellouin V, Lemaire M, Zaparucha A, Salanoubat M, de Berardinis V. Characterization of a thermotolerant ROK-type mannofructokinase from Streptococcus mitis: application to the synthesis of phosphorylated sugars. Appl Microbiol Biotechnol. 2018 Jul;102(13):5569–83. doi: 10.1007/s00253-018-9018-1.