CAS: 69227-93-6; (2R,3R,4S,5S,6R)-2-(((2R,3S,4R,5R,6R)-6-(Dodecyloxy)-4,5-Dihydroxy-2-(Hydroxymethyl)Tetrahydro-2H-Pyran-3-yl)Oxy)-6-(Hydroxymethyl)Tetrahydro-2H-Pyran-3,4,5-Triol

上下游产品

1-dodecyl alcohol α-hepta-O-acetylmaltosyl bromide 2,3,6,2',3',4',6'-hepta-O-acetyl-α-D-maltosyl bromide 1-dodecyl-2,3,6-tri-O-acetyl-4-O-(2,3,4,6-tetra-O-acetyl-α-D-glucopyranosyl)-β-D-glucopyranosiden-dodecyl-b-D-glucopyranoside 24H46O11*C47H56N8C24H46O11*C47H56N8 24H46O11*C51H64N8C24H46O11*C51H64N8

麦芽糖置于高氯酸,磷化氢,溴,碘,溶剂黄146,Silver Carbonate体系中,用 二氯甲烷 作为反应溶剂,化学反应 16.5H,反应生成 十二烷基-β-D-麦芽糖苷
参考文献:Synthesis Of Uniformly Deuteratedn-Dodecyl-β-D-Maltoside (D39-Ddm) For Solubilization Of Membrane Proteins In Trosy NMR Experiments
标题:Synthesis Of Uniformly Deuteratedn-Dodecyl-β-D-Maltoside (D39-Ddm) For Solubilization Of Membrane Proteins In Trosy NMR Experiments
摘要:这项工作报告了均匀氘标记的n-十二烷基-β-D-麦芽糖苷(d39-Ddm)的首次合成.ddm是一种温和的非离子型洗涤剂,通常用于膜蛋白的提取和纯化,以及在实验研究其结构,动力学和配体结合时的溶解.我们需要d39-Ddm来溶解样品中的大型α-螺旋膜蛋白,以便进行[15N-1H]Trosy(横向弛豫优化光谱)NMR实验,从而实现最高的灵敏度和最佳的光谱分辨率.我们的d39-Ddm合成使用了d7-D-葡萄糖和d25-N-十二醇,将氘标记引入麦芽糖苷和十二烷基基团中.两个葡萄糖分子,其中一个转化为具有自由c4羟基的糖苷受体,另一个转化为在c1位置被α-构型溴取代的糖苷供体,通过α(1->4)糖苷键结合在一起,形成麦芽糖,再通过用α-构型的c1溴取代将其与n-十二醇结合,得到ddm.利用1H NMR谱确认了合成的d39-Ddm具有较高的氘标记水平,并展示其在消除52-Kda糖转运蛋白在ddm中溶解的trosy NMR谱中的干扰信号方面的应用.
DOI:10.1002/jlcr.3249

专利信息

专利号:US-2012190064-A1
优先权日:2004-10-01
标题 :Feeding buffers, systems, and methods for in vitro synthesis of biomolecules
发明人：KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA
权利人:KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA; LIFE TECHNOLOGIES CORP
摘要:Compositions, methods and kits for in vitro systems for synthesis of biomolecules such as polypeptides, are provided herein. Cell extracts that provide enhanced yields of soluble proteins using in vitro protein synthesis methods are provided. The invention also includes methods for producing high yields of proteins by the addition of a feeding solution that includes amino acids and an energy source to an ongoing in vitro synthesis system. The invention also includes methods of using a high-yield in vitro synthesis system to produce large quantities of proteins with incorporated labeled amino acids for analysis by methods such as by NMR. The invention further includes vectors for enhanced production of proteins from nucleic acid templates using in vitro synthesis systems.

专利号:US-6451543-B1
优先权日:1998-08-31
标题:Lipid matrix-assisted chemical ligation and synthesis of membrane polypeptides
发明人：KOCHENDOERFER GERD G; HUNTER CHRISTIE L; KENT STEPHEN B H; BOTTI PAOLO
权利人:GRYPHON SCIENCES
摘要:The present invention relates to methods and compositions for lipid matrix-assisted chemical ligation and synthesis of membrane polypeptides that are incorporated in a lipid matrix. The invention is exemplified in production of a prefolded membrane polypeptide embedded within a lipid matrix via stepwise chemoselective chemical ligation of unprotected peptide segments, where at least one peptide segment is embedded in a lipid matrix. Any chemoselective reaction chemistry amenable for ligation of unprotected peptide segments can be employed. Suitable lipid matrices include liposomes, micelles, cell membrane patches and optically isotropic cubic lipidic phase matrices. Prefolded synthetic and semi-synthetic membrane polypeptides synthesized according to the methods and compositions of the invention also permit site-specific incorporation of one or more detectable moieties, such as a chromophore, which can be conveniently introduced during synthesis. The methods and compositions of the invention have multiple uses. For example, they can be used to assay ligand binding to membrane polypeptides and domains comprising a receptor, and thus are extremely useful for structure/function studies, drug screening/selection/design, and diagnostics and the like, including high-throughput applications. The methods and compositions of the invention are particularly suited for FRET analyses of previously inaccessible membrane polypeptides.

专利号:US-2012208232-A1
优先权日:2009-07-15
标 题:Cell-Free Synthesis of Active Reprogramming Transcription Factors
发明人：YANG WILLIAM; PATEL KEDAR; GHEBREMARIAM YOHANNES T; LEE JI EUN; WONG HANN-CHUNG; COOKE JOHN P; SWARTZ JAMES ROBERT
权利人:YANG WILLIAM; PATEL KEDAR; GHEBREMARIAM YOHANNES T; LEE JI EUN; WONG HANN-CHUNG; COOKE JOHN P; SWARTZ JAMES ROBERT
摘要:Compositions and methods are provided for the cell-free synthesis of active reprogramming factor polypeptides. The reprogramming factors may be synthesized as fusion proteins comprising a permeant domain, such as polyarginme. The cell free-synthesis may be conducted at about 25 C in a bacterial cell extract from genetically alterd cells having decreased endogenous protease activity Further, the proteins may comprise a fusion partner which enhances solubility and may be refolded on a column.

专利号:US-2001049117-A1
优先权日:1998-08-20
标 题 :Analogs of udp-murnac peptides, assays, kits and related methods of their use
发明人：AXELROD HELENA R; BRANSTROM ARTHUR A
摘要:General compositions and methods for detecting Lipid I, Lipid II and peptidoglycan synthesis is disclosed. A method of screening for potential antibacterial agents is provided which requires bacterial membrane preparations or enriched enzyme preparations including at least one bacterial enzyme involved in the synthesis of Lipid I from UDP-MurNAc pentapeptide and undecaprenyl phosphate, at least one bacterial enzyme involved in the synthesis of Lipid II from Lipid I and UDP-GlcNAc and one or more bacterial enzymes involved in the further processing of Lipid II toward the downstream synthesis of peptidoglycan. The methods disclosed herein further provides a labeled UDP-MurNAc-peptide capable of serving as a substrate for a bacterial enzyme involved in the synthesis of Lipid I and a labeled UDP-GlcNAc capable of serving as a substrate for a bacterial enzyme involved in the synthesis of Lipid II. Conditions for further processing of Lipid II toward the downstream synthesis of peptidoglycan are also discribed.

专利号:US-2011195450-A1
优先权日:2005-09-27
标 题 :In Vitro Protein Synthesis Systems for Membrane Proteins that Include Adolipoproteins and Phospholipid Adolipoprotein Particles
发明人：KUDLICKI WIESLAW; FLETCHER JULIA; KATZEN FEDERICO
权利人:LIFE TECHNOLOGIES CORP
摘要:In vitro protein synthesis systems and methods are provided that produce membrane proteins in soluble form. In some aspects, the invention provides methods of synthesizing proteins using in vitro protein synthesis systems that include an apolipoprotein, in which higher yields of soluble protein are produced than in the absence of the apolipoprotein. Apolipoproteins useful in the present invention include naturally occurring apolipoproteins, as well as sequence variants of wild-type apolipoproteins, and engineered apolipoproteins. The apolipoproteins can be provided in an in vitro protein synthesis system associated with lipid or not associated with lipid. The invention also provides compositions and kits for synthesis of proteins in soluble form, in which the compositions and kits include cell extracts for protein translation and at least one apolipoprotein biomolecule.

专利号:WO-2007038755-A1
优先权日:2005-09-27
标 题:In vitro protein synthesis systems for membrane proteins that include apolipoproteins and phospholipid-apolipoprotein particles
发明人：KUDLICKI WIESLAW; FLETCHER JULIA; KATZEN FEDERICO
权利人:INVITROGEN CORP; KUDLICKI WIESLAW; FLETCHER JULIA; KATZEN FEDERICO
摘要:In vitro protein synthesis systems and methods are provided that produce membrane proteins in soluble form. In some aspects, the invention provides methods of synthesizing proteins using in vitro protein synthesis systems that include an apolipoprotein, in which higher yields of soluble protein are produced than in the absence of the apolipoprotein. Apolipoproteins useful in the present invention include naturally occurring apolipoproteins, as well as sequence variants of wild-type apolipoproteins, and engineered apolipoproteins. The apolipoproteins can be provided in an in vitro protein synthesis system associated with lipid or not associated with lipid. The invention also provides compositions and kits for synthesis of proteins in soluble form, in which the compositions and kits include cell extracts for protein translation and at least one apolipoprotein biomolecule.