CAS: 68399-81-5; 3-[n-Tris(Hydroxymethyl)Methylamino]-2-Hydroxypropanesulfonic Acid

相似化合物

29915-38-6 73463-39-5 68399-80-4

专利信息

专利号:US-10501782-B1
优先权日:2014-09-05
标题:Cell-free synthesis of DNA by strand displacement
发明人：PORTER NEIL; ROTHWELL PAUL; EXTANCE JONATHAN
权利人:TOUCHLIGHT IP LTD
摘要:The present invention relates to an improved process for synthesis of DNA, RNA, proteins and like molecules, in particular cell-free enzymatic synthesis of DNA, preferably in large scale. The present invention relates to the synthesis of DNA using strand-displacement replication and the addition of nucleotides to the reaction mixture is controlled, thus controlling yield. The reaction mixture contains a starting amount of nucleotides, polymerase and DNA template, to which further nucleotides are supplied in a controlled manner.

专利号:US-2019323050-A1
优先权日:2016-12-21
标题 :Modulation of Enzymatic Polynucleotide Synthesis Using Chelated Divalent Cations
发明人：GRISWOLD JR KETTNER JOHN FREDERICK; LEE HOWON; CHURCH GEORGE M
权利人:HARVARD COLLEGE
摘要:Methods and apparatus of modulating polynucleotide synthesis are provided. The methods include delivering reagents comprising enzymes, nucleotides and ions to oligonucleotide primers wherein the reagents catalyze incorporation of the nucleotides to 3′ ends of the oligonucleotide primers, and modulating incorporation of the nucleotides to the 3′ ends of the oligonucleotide primers. Polynucleotide synthesis is modulated by modulating presence or absence of catalytic cation cofactors to provide sequence defined synthesis of polynucleotides. In certain embodiments, the polynucleotides encode information.

专利号:US-2025136636-A1
优先权日:2021-08-16
标 题:Nucleotide complexes capable of an improved dna synthesis yield
发明人：KYSH DANIEL; ROTHWELL PAUL
权利人:TOUCHLIGHT IP LTD
摘要:The present invention relates to novel nucleotide complexes which enhance the enzymatic production of DNA. The nucleotide complexes includes a divalent cation at a ratio of 0.2-1.5 divalent cations per nucleotide, whilst monovalent ions (other than hydrogen or hydronium ions from any solvent) are minimal. The nucleotide complexes have desirable properties for the synthesis of deoxyribonucleic acid (DNA), in particular cell-free enzymatic synthesis of DNA, preferably on a large or industrial scale. Further, the invention includes improved processes for preparing said nucleotide complexes. Further complexes are disclosed which possess unique properties in accelerating DNA synthesis at higher concentrations, these complexes also containing zwitterion molecules.

专利号:US-2023091493-A1
优先权日:2020-02-14
标 题:Dna synthesis yield improvements
发明人：ROTHWELL PAUL; KYSH DANIEL; PORTER NEIL
权利人:TOUCHLIGHT IP LTD
摘要:The present invention relates to an improved process for synthesis of deoxyribonucleic acid (DNA), in particular cell-free enzymatic synthesis of DNA, preferably on a large or industrial scale, with an improved yield and/or with an improved efficiency. The invention requires the use of nucleotide complexes wherein the nucleotide is associated with a mixture of divalent and monovalent cations. Preferably, the divalent cation may be magnesium or manganese.

专利号:WO-2024153643-A1
优先权日:2023-01-16
标 题 :Inkjet-assisted enzymatic nucleic acid synthesis
发明人：MOGHADDAM NICOLAS; ADELIZZI BEATRICE; LOMAN TESSA; VERARDO DAMIANO; RODRIGUEZ-PINZON DANIEL; HORGAN ADRIAN
权利人:DNA SCRIPT
摘要:Methods, devices, and compositions are provided for inkjet-assisted synthesis of a plurality of polynucleotides at reaction sites on a substrate using template-free polymerases, such as, terminal deoxynucleotidyl transferases (TdTs). Compositions include printable stabile formulations of synthesis reagents for inkjet delivery including, but not limited to, template-free polymerases, divalent cations, and nucleotides.

专利号:US-2022233673-A1
优先权日:2019-06-04
标 题:METHODS OF PRODUCING SHIGA TOXIN B-SUBUNIT (STxB) MONOMERS AND OLIGOMERS, AND USES THEREOF
发明人：BILLET ANNE; SCHMIDT FRÉDÉRIC; JOHANNES LUDGER; SERVENT DENIS; MOURIER GILLES; TARTOUR ÉRIC; KAY MICHAEL; FULCHER JAMES M
权利人:INST CURIE; CENTRE NAT RECH SCIENT; INST NAT SANTE RECH MED; COMMISSARIAT A LENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES CEA; APHP ASSIST PUBLIQUE HOPITAUX DE PARIS; UNIV PARIS; UNIV OF UTAH RESEARCH FOUDATION; UNIV UTAH RES FOUND
摘要:A method of producing a monomer of a Shiga toxin B-subunit (STxB) protein or of a variant thereof by peptide chemical synthesis, as well as to a method of producing a pentamer of the STxB protein or of the variant thereof. The methods are particularly advantageous as they overcome major issues typically observed in peptide chemical synthesis, including solubility and purity issues.