专利号:US-2003224420-A1 优先权日:2002-04-05 标题 :Method for isolating and measuring proliferation of long-term label retaining cells and stem cells 发明人:HELLERSTEIN MARC K; KIM SYLVIA JEEWON 摘要:This invention relates to a method for separating long-term label retaining cells or stem cells. In particular, this invention relates to a method for separating long-term label retaining cells and/or stem cells from tissues or individuals and for measuring proliferation rates of long-term label retaining cells and stem cells, as well as determining clonal expansion (proliferative history) of cell lineages from the tissues of the individual. The cells may be double-labeled with a cell-lineage marking label and isotopically labeled DNA synthesis precursor prior to physical separation.
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